Classification of anaerobic gut fungi from herbivores with emphasis on rumen fungi from Malaysia

Ho, Yin Wan; Barr, D. J. S.

doi:10.1080/00275514.1995.12026582

Cited by 57 publications

(83 citation statements)

References 36 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Morphologically these new isolates conformed most closely to members of the genus Piromyces, in which nine species have been described (Ho & Barr 1995;Ho et al 1993a, b;Kirk 2012). However, of these Piromyces species, none of the type specimens for these species have been subject to both morphological and genetic analysis, except the rather distinctive Piromyces polycephalus (recently renamed as Anaeromyces polycephalus (Chen et al 2002;Kirk 2012)).…”

Section: Resultsmentioning

confidence: 77%

“…Intercalary rhizoidal swellings were frequently observed (Fig 1F, G); these swelling bore some resemblance to the intercalary sporangia reported in Orpinomyces intercalaris (Ho & Barr 1995;Dagar et al 2011) but none was ever seen to release or contain zoospores. Thus colony morphology was consistently monocentric (single sporangium per thallus) but confirmation using DAPI-staining and fluorescent microscopy that nuclei were restricted to sporangia (Ozkose et al 2001) was not conducted.…”

Section: Resultsmentioning

confidence: 90%

“…Since their belated recognition as Fungi (Orpin 1974), some 20 species have been reported (Griffith et al 2009;Sirohi et al 2012) but the taxonomic status of some of these species is uncertain (Ho & Barr 1995;Ozkose et al 2001;Hibbett et al 2007;Eckart et al 2010). Following revision of the broader taxonomy of kingdom Fungi, this group is now considered as phylum Neocallimastigomycota, containing a single family, Neocallimastigaceae (in the order Neocallimastigales) (Hibbett et al 2007).…”

Section: Introductionmentioning

confidence: 97%

“…The six genera within Neocallimastigomycota are divided into two groups based on their growth patterns: monocentric (Neocallimastix, Piromyces, and Caecomyces) or polycentric (Orpinomyces, Anaeromyces, and Cyllamyces), with the former growing as determinate thalli with a single sporangium and the latter forming more complex thalli with multiple sporangia (Ho & Barr 1995;Griffith et al 2009). Two genera (Neocallimastix and Orpinomyces) form zoospores with multiple (7e30) flagella, in contrast to the uniflagellate zoospores of all other zoosporic fungi.…”

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

A new anaerobic fungus (Oontomyces anksri gen. nov., sp. nov.) from the digestive tract of the Indian camel (Camelus dromedarius)

et al. 2015

View full text Add to dashboard Cite

Document embargo until 01/05/2016Two cultures of anaerobic fungi were isolated from the forestomach of an Indian camel (Camelus dromedarius). Phylogenetic analysis using both the internal transcribed spacer (ITS) and large-subunit (LSU) regions of the rRNA locus demonstrated that these isolates were identical and formed a distinct clade within the anaerobic fungi (phylum Neocallimastigomycota). Morphological examination showed that these fungi formed monocentric thalli with filamentous rhizoids and uniflagellate zoospores, broadly similar to members of the genus Piromyces. However, distinctive morphological features were observed, notably the pinching of the cytoplasm in the sporangiophore and the formation of intercalary rhizoidal swellings. Since genetic analyses demonstrated this fungus was only distantly related to Piromyces spp. and closer to the polycentric Anaeromyces clade, we have assigned it to a new genus and species Oontomyces anksri gen. nov., sp. nov. Interrogation of the GenBank database identified several closely related ITS sequences, which were all environmental sequences obtained from camels, raising the possibility that this fungus may be specific to camelids.authorsversionPeer reviewe

show abstract

Section: Resultsmentioning

confidence: 77%

Section: Resultsmentioning

confidence: 90%

Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A new anaerobic fungus (Oontomyces anksri gen. nov., sp. nov.) from the digestive tract of the Indian camel (Camelus dromedarius)

et al. 2015

View full text Add to dashboard Cite

show abstract

“…Due to their strict anaerobic nature [2], the propensity of many strains for senescence upon continuous subculture [3], and the lack of reliable long-term storage and maintenance procedures [4], progress on various aspects of AGF research has lagged behind their aerobic counterparts. For example, development of molecular biological approaches for genetic manipulations in the AGF (e.g.…”

Section: Introductionmentioning

confidence: 99%

A fast and reliable procedure for spore collection from anaerobic fungi: Application for RNA uptake and long-term storage of isolates

Calkins

Elledge

Hanafy

et al. 2016

Journal of Microbiological Methods

View full text Add to dashboard Cite

Anaerobic gut fungi (AGF) represent a basal fungal lineage (phylum Neocallimastigomycota) that resides in the rumen and alimentary tracts of herbivores. The AGF reproduce asexually, with a life cycle that involves flagellated zoospores released from zoosporangia followed by encystment, germination and the subsequent development of rhizomycelia. A fast and reliable approach for AGF spore collection is critical not only for developmental biology studies, but also for molecular biological (e.g. AMT-transformation and RNAi) approaches. Here, we developed and optimized a simple and reliable procedure for the collection of viable, competent, and developmentally synchronized AGF spores under strict anaerobic conditions. The approach involves growing AGF on agar medium in serum bottles under anaerobic conditions, and flooding the observed aerial growth to promote spore release from sporangia into the flooding suspension. The released spores are gently collected using a wide bore sterile needle. Process optimization resulted in the recovery of up to 7×10(9) spores per serum bottle. Further, the released spores exhibited synchronized development from flagellated spores to encysted spores and finally to germinating spores within 90min from the onset of flooding. At the germinating spore stage, the obtained spores were competent, and readily uptook small interfering RNA (siRNA) oligonucleotides. Finally, using multiple monocentric and polycentric AGF isolates, we demonstrate that AGF grown on agar surface could retain viability for up to 16weeks at 39°C, and hence this solid surface growth procedure represents a simple, cryopreservative- and freezing temperature-free approach for AGF storage.

show abstract

Anaerobic gut fungi: Advances in isolation, culture, and cellulolytic enzyme discovery for biofuel production

Haitjema

Solomon

Henske

et al. 2014

Biotech & Bioengineering

115

View full text Add to dashboard Cite

Anaerobic gut fungi are an early branching family of fungi that are commonly found in the digestive tract of ruminants and monogastric herbivores. It is becoming increasingly clear that they are the primary colonizers of ingested plant biomass, and that they significantly contribute to the decomposition of plant biomass into fermentable sugars. As such, anaerobic fungi harbor a rich reservoir of undiscovered cellulolytic enzymes and enzyme complexes that can potentially transform the conversion of lignocellulose into bioenergy products. Despite their unique evolutionary history and cellulolytic activity, few species have been isolated and studied in great detail. As a result, their life cycle, cellular physiology, genetics, and cellulolytic metabolism remain poorly understood compared to aerobic fungi. To help address this limitation, this review briefly summarizes the current body of knowledge pertaining to anaerobic fungal biology, and describes progress made in the isolation, cultivation, molecular characterization, and long-term preservation of these microbes. We also discuss recent cellulase- and cellulosome-discovery efforts from gut fungi, and how these interesting, non-model microbes could be further adapted for biotechnology applications.

show abstract

Classification of anaerobic gut fungi from herbivores with emphasis on rumen fungi from Malaysia

Cited by 57 publications

References 36 publications

A new anaerobic fungus (Oontomyces anksri gen. nov., sp. nov.) from the digestive tract of the Indian camel (Camelus dromedarius)

A new anaerobic fungus (Oontomyces anksri gen. nov., sp. nov.) from the digestive tract of the Indian camel (Camelus dromedarius)

A fast and reliable procedure for spore collection from anaerobic fungi: Application for RNA uptake and long-term storage of isolates

Anaerobic gut fungi: Advances in isolation, culture, and cellulolytic enzyme discovery for biofuel production

Contact Info

Product

Resources

About