Classification of Corynebacterium Species from Axillary Skin by Numerical Analysis of Electrophoretic Protein Patterns

Characterization of Corynebacterium Group JK by Whole-cell Protein Patterns

“…Since many of the strains had been identified only by their coryneform morphology and antibiotic resistance, the high level of homogeneity observed was remarkable, particularly in view of the diversity amongst both reference Corynebacterium spp. and isolates from human skin found in a previous study (Jackman, 1982). The level of similarity C50l c502 c471 c473 C218 C 5 l l C504 C512 C513 c494 c225 C467 C440 C477 c444 C420 C426 C422 c433 C226 C417 c493 c222 C22l C227 C416 c205 C207 C472 C4Y7 C498 C429 C487 C489 c545 among the group JK strains was similar to that between reference strains of C. diphtheriae (Jackman, 1982), which confirms the coherence of the group, as found by Riley et al (1979).…”

“…and isolates from human skin found in a previous study (Jackman, 1982). The level of similarity C50l c502 c471 c473 C218 C 5 l l C504 C512 C513 c494 c225 C467 C440 C477 c444 C420 C426 C422 c433 C226 C417 c493 c222 C22l C227 C416 c205 C207 C472 C4Y7 C498 C429 C487 C489 c545 among the group JK strains was similar to that between reference strains of C. diphtheriae (Jackman, 1982), which confirms the coherence of the group, as found by Riley et al (1979). All group JK patterns were characterized by a prominent band of about 45 kDa; however, there was much fine strain variation particularly in the high molecular mass range of the gel.…”

Coryneform bacteria in infectious diseases: clinical and laboratory aspects

“…British microbiologists who have studied the flora of human skin have deplored the inadequacies of the existing coryneform taxonomy (57,132,157,162,197). Even when skin isolates do belong in the genus Corynebacterium, they usually are not members of any recognized species (91). Although the CDC identification scheme for gram-positive rods (87) is an invaluable resource, most clinical laboratories that serve immunocompromised patients continue to encounter difficulties with the identification of coryneform isolates.…”

“…The remaining isolates could not be assigned to a genus. Jackman subsequently analyzed the whole-cell protein patterns of 40 axillary skin isolates that were presumed to be Corynebacterium species on the basis of Pitcher's cell wall analyses (91). Amazingly, none of the isolates could be grouped with any of the 40 reference strains representing 18 different Corynebacterium species!…”

The use of SDS-polyacrylamide gel electrophoresis in epidemiological studies ofCorynebacterium diphtheriae

“…Polyacrylamide gel electrophoresis of cell proteins in the presence of sodium dodecyl sulphate (SDS-PAGE) was investigated because Larsen et al (1971) found that this method gave distinct patterns for different serotypes of C. diphtheriae. Also this technique has been used by Jackman (1982Jackman ( , 1986 (Robinson & Peeney, 1936) were obtained from the National Collection of Type Cultures, Central Public Health Laboratory.…”