Characterization of Corynebacterium Group JK by Whole-cell Protein Patterns

Jackman, P. J. H.; Pelczynska, Stephanie

doi:10.1099/00221287-132-7-1911

Cited by 15 publications

(12 citation statements)

References 7 publications

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“…(14). Our study confirmed the heterogeneity of whole cell protein pattern in the bacterial strains of various genera, as reported previously (13,14). However, homogeneity of major protein profiles was observed in strains of the same species.…”

Section: Discussionsupporting

confidence: 94%

Protein patterns, serotyping and plasmid DNA profiles in the epidemiologic fingerprinting ofPseudomonas aeruginosa

Walia

Madhavan

Williamson

et al. 1988

Eur. J. Clin. Microbiol. Infect. Dis.

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The epidemiological typing schemes based on serotyping, antibiotic susceptibility pattern, plasmid DNA profile, and protein patterns determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) were evaluated for their usefulness in typing clinical isolates of Pseudomonas aeruginosa. The serotypability was lower (45%) than reported in the literature (85-95%). The most commonly found serotypes were O:1 (19%), O:11 (25%), O:6 (35%). The electrophoretic analysis of plasmid DNAs showed plasmids (molecular weight = 1 to greater than 40 megadaltons). Two plasmids of Mr 2 and 38 megadaltons were found in various serotypes. The restriction enzyme analysis of plasmid DNA showed identical DNA fragment patterns among distinct serotypes. The SDS-PAGE protein banding patterns of whole-cell proteins showed homogeneity among the strains. However, analysis of the soluble protein patterns of the strains showed sufficiently distinct protein profiles that can be used to differentiate between various strains. The results of this study demonstrate that the electrophoretic patterns of soluble proteins, in combination with plasmid DNA profile or serotyping, can be of value in the epidemiologic fingerprinting of clinical isolates of Pseudomonas aeruginosa.

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Section: Discussionsupporting

confidence: 94%

Protein patterns, serotyping and plasmid DNA profiles in the epidemiologic fingerprinting ofPseudomonas aeruginosa

Walia

Madhavan

Williamson

et al. 1988

Eur. J. Clin. Microbiol. Infect. Dis.

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“…The most recent approach to classification of coryneforms is the reverse transcriptase sequencing of 16S rRNA, which allows the sequencing of over 1,000 nucleotides (38,39). These long (2,20,29,48,54,63,79,92,104,105,129,161,165,221,238,239,242). The paucity of reports of C. jeikeium infections in Great Britain, compared with the United States, may be due to differences in identification procedures (106).…”

Section: Introductionmentioning

confidence: 99%

Coryneform bacteria in infectious diseases: clinical and laboratory aspects

1990

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Coryneform isolates from clinical specimens frequently cannot be identified by either reference laboratories or research laboratories. Many of these organisms are skin flora that belong to a large number of taxonomic groups, only 40% of which are in the genus Corynebacterium. This review provides an update on clinical presentations, microbiological features, and pathogenic mechanisms of infections with nondiphtheria Corynebacterium species and other pleomorphic gram-positive rods. The early literature is also reviewed for a few coryneforms, especially those whose roles as pathogens are controversial. Recognition of newly emerging opportunistic coryneforms is dependent on sound identification schemes which cannot be developed until cell wall analyses and nucleic acid studies have defined the taxonomic groups and all of the reference strains within each taxon have been shown by molecular methods to be authentic members. Only then can reliable batteries of biochemical tests be selected for distinguishing each taxon.

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“…Polyacrylamide gel electrophoresis of cell proteins in the presence of sodium dodecyl sulphate (SDS-PAGE) was investigated because Larsen et al (1971) found that this method gave distinct patterns for different serotypes of C. diphtheriae. Also this technique has been used by Jackman (1982Jackman ( , 1986 (Robinson & Peeney, 1936) were obtained from the National Collection of Type Cultures, Central Public Health Laboratory.…”

Section: Introductionmentioning

confidence: 99%