Classification of Ralstonia pickettii biovar 3/'thomasii' strains (Pickett 1994) and of new isolates related to nosocomial recurrent meningitis as Ralstonia mannitolytica sp. nov.

Baère, Thierry De; Steyaert, Sophia; Wauters, Georges; Vos, Paul De; Goris, Johan; Coenye, Tom; Suyama, Tetsushi; Verschraegen, Gerda; Vaneechoutte, Mario

doi:10.1099/00207713-51-2-547

Cited by 92 publications

(85 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Reactions in the API20NE system (bioMérieux) were positive for gluconate (n ϭ 5), caprate (n ϭ 5), and malate (n ϭ 5) and were variable for adipate (n ϭ 3) and citrate (n ϭ 1). This biochemical profile was consistent with an identification as R. gilardii (2,3). It should be mentioned that all strains were found to have multiperitrichous flagella instead of a single polar flagellum as described previously (2).…”

supporting

confidence: 68%

Case of Catheter Sepsis with Ralstonia gilardii in a Child with Acute Lymphoblastic Leukemia

Wauters

Claeys²,

Verschraegen³

et al. 2001

J Clin Microbiol

View full text Add to dashboard Cite

Acute lymphoblastic leukemia was diagnosed in a 7-year-old girl. Two months after insertion of a central venous catheter, she developed fever and complained of headache and abdominal pain. Physical examination revealed no focus of infection. A gram-negative nonfermenting bacillus was recurrently cultured from blood. Extensive biochemical testing and 16S ribosomal DNA sequencing led to the identification of Ralstonia gilardii. CASE REPORTAcute lymphoblastic leukemia (ALL) was diagnosed in a 7-year-old girl in May 2000, and treatment was initiated according to the EORTC-CLCG-58951 protocol for children with very-low-risk ALL. The girl achieved hematologic remission after induction chemotherapy. A central venous catheter was inserted in June 2000. The girl tolerated the treatment uneventfully until September 2000, when, during a course of chemotherapy (high-dose methotrexate), she developed spiking fever as high as 40°C. She complained of headache and abdominal pain and vomited twice. Physical examination revealed no focus of infection. The leucocyte count was 5,800/ml, with an absolute neutrophil count of 4,760/ml and elevated C-reactive protein (87 mg/dl). The chemotherapy was stopped, and the girl was treated with intravenous (i.v.) ampicillin (100 mg/kg of body weight/day). One day later blood cultures grew gram-negative bacilli, and (i.v.) netromycin (7.5 mg/kg/day) treatment was added. The spiking fever disappeared and the girl's health improved.A gram-negative nonfermenting bacillus was isolated and found to be resistant to ampicillin, piperacillin, aztreonam, gentamicin, and tobramycin and susceptible to cefuroxime, ceftriaxone, ceftazidime, imipenem, co-trimoxazole, ofloxacin, and amikacin. The girl was treated as an outpatient with i.v. ceftriaxone (100 mg/kg/day) once daily for 4 more days. Thirtysix hours after the ceftriaxone treatment was stopped, she again developed spiking fever, and i.v. ceftriaxone (100 mg/kg/ day) was restarted in combination with i.v. amikacin (15 mg/ kg/day). Again, a gram-negative nonfermenting bacillus was cultured from blood. The girl showed an allergic reaction to ceftriaxone with rash and pruritus, and the ceftriaxone was replaced with i.v. ciprofloxacin (20 mg/kg/day). The spiking fever disappeared again, and amikacin and ciprofloxacin i.v. treatment was given for 7 more days. Blood cultures remained negative, and the central venous catheter was not removed. Three months later the intensive chemotherapy was completed and the girl was doing well. The catheter was removed on January 8 2001. No nonfermenting gram-negative bacilli were cultured from the tip.Discussion. The gram-negative bacillus was isolated in pure culture from all eight FAN aerobic blood cultures and from one of the eight BacT/Alert anaerobic cultures (Organon Teknika, Turnhout, Belgium), collected over a period of 10 days. Initial identification based on the API20NE system (bioMérieux, Marcy l'Etoile, France) yielded code 1000474, leading to an identification as Alcaligenes faecalis, Comamonas acidovorans ...

show abstract

supporting

confidence: 68%

Case of Catheter Sepsis with Ralstonia gilardii in a Child with Acute Lymphoblastic Leukemia

Wauters

Claeys²,

Verschraegen³

et al. 2001

J Clin Microbiol

View full text Add to dashboard Cite

show abstract

“…The R. insidiosa strains used in this study, several of which have been deposited in the culture collections of the BCCM/LMG (Laboratorium voor Microbiologie Gent, Belgium) and CCUG (University of Göteborg, Department of Clinical Bacteriology, Göteborg, Sweden), are listed in Table 1. Type and reference strains of all other Ralstonia species have been described previously Vandamme et al, 1999;Chen et al, 2001;De Baere et al, 2001;Goris et al, 2001). Strains were grown aerobically on Mueller-Hinton broth (Becton Dickinson) supplemented with 1?8 % (w/v) agar and incubated for 24 h at 32 uC, unless otherwise indicated.…”

Section: Methodsmentioning

confidence: 99%

“…Ralstonia pickettii has been isolated from various clinical sources (Riley & Weaver, 1975;McNeil et al, 1985) and has been found to be responsible for pseudobacteraemia (Verschraegen et al, 1985). Strains of R. mannitolilytica (formerly known as 'Pseudomonas thomasii' and R. pickettii biovar 3) have also been isolated from various clinical sources, including nosocomial recurrent meningitis (Philips et al, 1972;De Baere et al, 2001;Vaneechoutte et al, 2001). R. gilardii, R. paucula and R. taiwanensis have been isolated both from human clinical samples and from the environment Vandamme et al, 1999;Chen et al, 2001;Wauters et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov.

Coenye

Goris

Vos

et al. 2003

International Journal of Systematic and Evolutionary Microbiology

116

View full text Add to dashboard Cite

“…5). These peptides are from the enzyme toluene-3-monooxygenase of the two very closely related bacteria strains Burkolderia cepacia and Ralstonia pickettii [25][26][27][28]. Both bacteria are ubiquitous in the environment but rarely cause symptomatic infection in healthy individuals [29].…”

Section: S a R W N T P T A M32253mentioning

confidence: 99%

Identification of peptides from human pathogens able to cross‐activate an HIV‐1‐gag‐specific CD4⁺ T cell clone

Venturini¹,

Allicotti²,

Zhao³

et al. 2005

Eur J Immunol

View full text Add to dashboard Cite

Antigen recognition by T cells is degenerate both at the MHC and the TCR level. In this study, we analyzed the cross‐reactivity of a human HIV‐1 gag p24‐specific CD4+ T cell clone obtained from an HIV‐1‐seronegative donor using a positional scanning synthetic combinatorial peptide library (PS‐SCL)‐based biometrical analysis. A number of decapeptides able to activate the HIV‐1 gag‐specific clone were identified and shown to correspond to sequences found in other human pathogens. Two of these peptides activated the T cell clone with the same stimulatory potency as the original HIV‐1 gag p24 peptide. These findings show that an HIV‐1‐specific human T helper clone can react efficiently with peptides from other pathogens and suggest that cellular immune responses identified as being specific for one human pathogen (HIV‐1) could arise from exposure to other pathogens.

show abstract

Classification of Ralstonia pickettii biovar 3/'thomasii' strains (Pickett 1994) and of new isolates related to nosocomial recurrent meningitis as Ralstonia mannitolytica sp. nov.

Abstract: Classification of

Cited by 92 publications

References 37 publications

Case of Catheter Sepsis with Ralstonia gilardii in a Child with Acute Lymphoblastic Leukemia

Case of Catheter Sepsis with Ralstonia gilardii in a Child with Acute Lymphoblastic Leukemia

Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov.

Identification of peptides from human pathogens able to cross‐activate an HIV‐1‐gag‐specific CD4⁺ T cell clone

Contact Info

Product

Resources

About

Classification of Ralstonia pickettii biovar 3/'thomasii' strains (Pickett 1994) and of new isolates related to nosocomial recurrent meningitis as Ralstonia mannitolytica sp. nov.

Abstract: Classification of

Cited by 92 publications

References 37 publications

Case of Catheter Sepsis with Ralstonia gilardii in a Child with Acute Lymphoblastic Leukemia

Case of Catheter Sepsis with Ralstonia gilardii in a Child with Acute Lymphoblastic Leukemia

Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov.

Identification of peptides from human pathogens able to cross‐activate an HIV‐1‐gag‐specific CD4+ T cell clone

Contact Info

Product

Resources

About

Identification of peptides from human pathogens able to cross‐activate an HIV‐1‐gag‐specific CD4⁺ T cell clone