Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov.

Coenye, Tom; Goris, Johan; Vos, Paul De; Vandamme, Peter; LiPuma, John J.

doi:10.1099/ijs.0.02555-0

Cited by 116 publications

(95 citation statements)

References 29 publications

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“…P. saccherophilia is known as ''knallgas bacterium'' and has the specific ability to hydrolyse starch and gelatin (Aragno and Schlegel 1992). R. pickettii (Coenye et al 2003) and A. butzleri are known as pathogen organisms of clinical sources . Unfortunately, these data do not allow a reliable interpretation of the ecological functions of intratunical bacteria.…”

Section: Discussionmentioning

confidence: 99%

Diversity of intratunical bacteria in the tunic matrix of the colonial ascidian Diplosoma migrans

et al. 2005

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This paper provides the first information on diversity based on sequence data of the 16S rDNA of intratunical bacteria in the colonial ascidian Diplosoma migrans and its embryonic offspring. Ascidians were collected from waters near Helgoland (German Bight, North Sea). Sample material comprised tunic tissue, bacteria collected from tunic tissue, eggs with single embryos at different developmental stages, and freeswimming larvae. Bacterial 16S rDNA from D. migrans was directly amplified using PCR. DNA species were separated using denaturing gradient gel electrophoresis (DGGE). DGGE profiles generated ca. ten different distinguishable operational taxonomic units. Eleven bands from different sample materials were successfully re-amplified and sequenced. Sequence data generated five different subgroups of intratunical proteobacteria. The dominant band, detected in all of the samples tested, showed a low degree of relationship (84-86%) to Ruminococcus flavefaciens (d-subgroup). A weaker band, located above, which was not detected in all of the samples, was also similarly related to R. flavefaciens. Other bands derived from tunic material and embryonic stages showed closer relationship (ca. 97-99%) to Pseudomonas saccherophilia, a knallgas bacterium, and Ralstonia pickettii, a pathogen bacterium (both members of the b-subgroup). A solitary band generated from tunic material was assigned to a typical marine Flavobacterium symbiont (95%). Finally, a band from isolated bacteria was related (96%) to pathogen Arcobacter butzleri (e-subgroup). At this state of the investigation, a reliable interpretation of the ecological functions of intratunical bacteria cannot yet be given. This is due to the low degree of relationship of some of the bacteria and the fact that not all of the characteristic bands were successfully sequenced. However, the intratunical bacteria represent a unique bacterial community. Their DGGE profiles do not correspond to the profiles of the planktonic bacteria generated from surface seawater close to the ascidian habitat. The allocation of DNA sequences to the different morphotypes, their isolation and culturing, and the elucidation of the physiological functions of intratunical bacteria are in progress.

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Section: Discussionmentioning

confidence: 99%

Diversity of intratunical bacteria in the tunic matrix of the colonial ascidian Diplosoma migrans

et al. 2005

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“…After submission of the first draft of this manuscript, phenotypically similar strains with nearly identical 16S rDNA sequences (see Fig. 1) were described as R. insidiosa (Coenye et al, 2003a). The strains of R. insidiosa studied here, some of which are highly clinically relevant, had one to three polar flagella, were glucolytic and were resistant to colistin; they therefore fit within the genus Ralstonia sensu stricto.…”

Section: R Insidiosamentioning

confidence: 99%

“…The type species of the genus -Ralstonia pickettii (type strain, ATCC 27511 T ) -was regarded originally as the only representative of clinical importance (Fass & Barnishan, 1976;Fujita et al, 1981;Kahan et al, 1983;Gardner & Shulman, 1984;Verschraegen et al, 1985;Roberts et al, 1990a;Lacey & Want, 1991;Dimech et al, 1993;Raveh et al, 1993). Recently, several novel species [Ralstonia basilensis (Steinle et al, 1998;Goris et al, 2001), Ralstonia campinensis , Ralstonia gilardii (Coenye et al, 1999), Ralstonia insidiosa (Coenye et al, 2003a), Ralstonia metallidurans , Ralstonia mannitolilytica corrig. ; original spelling, Ralstonia mannitolytica), Ralstonia oxalatica (Sahin et al, 2000), Ralstonia paucula (Osterhout et al, 1998;Vandamme et al, 1999;Moissenet et al, 1999), Ralstonia respiraculi (Coenye et al, 2003b) and Ralstonia taiwanensis (Chen et al, 2001)] have been described, some of which are of moderate clinical importance.…”

Section: Introductionmentioning

confidence: 99%

Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov.

Vaneechoutte

Kämpfer

Baère

et al. 2004

International Journal of Systematic and Evolutionary Microbiology

244

114

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Culture Collection of the University of Gøteborg, Gøteborg, SwedenComparative 16S rDNA sequence analysis indicates that two distinct sublineages, with a sequence dissimilarity of >4 % (bootstrap value, 100 %), exist within the genus Ralstonia: the Ralstonia eutropha lineage, which comprises Ralstonia basilensis, Ralstonia campinensis, R. eutropha, Ralstonia gilardii, Ralstonia metallidurans, Ralstonia oxalatica, Ralstonia paucula, Ralstonia respiraculi and Ralstonia taiwanensis; and the Ralstonia pickettii lineage, which comprises Ralstonia insidiosa, Ralstonia mannitolilytica, R. pickettii, Ralstonia solanacearum and Ralstonia syzygii comb. nov. (previously Pseudomonas syzygii ). This phylogenetic discrimination is supported by phenotypic differences. Members of the R. eutropha lineage have peritrichous flagella, do not produce acids from glucose and are susceptible to colistin, in contrast to members of the R. pickettii lineage, which have one or more polar flagella, produce acid from several carbohydrates and are colistin-resistant. Members of the R. pickettii lineage are viable for up to 6 days on tryptic soy agar at 25 6C, whereas members of the R. eutropha lineage are viable for longer than 9 days. It is proposed that species of the R. eutropha lineage should be classified in a novel genus, Wautersia gen. nov. Finally, based on the literature and new DNA-DNA hybridization data, it is proposed that Pseudomonas syzygii should be renamed Ralstonia syzygii comb. nov. INTRODUCTIONThe genus Ralstonia (Yabuuchi et al., 1995) was created to accommodate bacteria from ecologically diverse niches that were classified previously as Burkholderia (Yabuuchi et al., 1992) and Alcaligenes. The type species of the genus -Ralstonia pickettii (type strain, ATCC 27511 T ) -was regarded originally as the only representative of clinical importance (Fass & Barnishan, 1976;Fujita et al., 1981;Kahan et al., 1983;Gardner & Shulman, 1984;Verschraegen et al., 1985;Roberts et al., 1990a;Lacey & Want, 1991; Dimech et al., 1993;Raveh et al., 1993) In a previous study , it was indicated that species of the genus Ralstonia can be separated clearly into two phenotypically and genotypically distinct groups. Here, it is proposed to consolidate these findings by allocating one group of species to a novel genus, Wautersia The results of lactose and maltose acidification by Ralstonia pickettii strains, the 16S rRNA gene signature sequences of the Ralstonia species, detailed phenotypic data of different species and a similarity matrix of the 16S rDNA sequences are available as supplementary material (Tables A1, A2, A3 and A4, respectively) in IJSEM Online.

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“…These values did not reveal relatedness at the species level for any of these pairs. Strains 1245 T and 1263a T and the type strains of established Cupriavidus species were further compared by SDS-PAGE of whole-cell proteins, a method that has often been proven to differentiate at the species level (Pot et al, 1994;Kersters & De Ley, 1975): indeed, in the genera Ralstonia and Cupriavidus, high protein electrophoretic similarity correlates with a high level of DNA-DNA hybridization (Coenye et al, , 2003aVandamme et al, 1999;Goris et al, 2001;Vandamme & Coenye, 2004). UPGMA cluster analysis of SDS-PAGE protein profiles showed that strain 1245 T and Ralstonia sp.…”

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confidence: 99%

Cupriavidus pinatubonensis sp. nov. and Cupriavidus laharis sp. nov., novel hydrogen-oxidizing, facultatively chemolithotrophic bacteria isolated from volcanic mudflow deposits from Mt. Pinatubo in the Philippines

Sato

Nishihara

Yoshida

et al. 2006

International Journal of Systematic and Evolutionary Microbiology

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Taxonomic studies were performed on ten hydrogen-oxidizing, facultatively chemolithotrophic bacteria that were isolated from volcanic mudflow deposits derived from the eruption of Mt. Pinatubo in the Philippines in 1991. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these isolates belonged to the genus Cupriavidus of the Betaproteobacteria; sequence similarity values with their nearest phylogenetic neighbour, Cupriavidus basilensis, were 97.1–98.3 %. In addition to phylogenetic analysis, results of whole-cell protein profiles and biochemical tests revealed that these strains were members of two distinct species. DNA–DNA hybridizations and whole-cell protein profiles enabled these isolates to be differentiated from related Cupriavidus species with validly published names. The isolates were aerobic, Gram-negative, non-sporulating, peritrichously flagellated rods. Their G+C contents ranged from 65.2 to 65.9 mol% and their major isoprenoid quinone was ubiquinone Q-8. On the basis of these results, two novel species are proposed, Cupriavidus pinatubonensis sp. nov. [nine strains, with 1245T (=CIP 108725T=PNCM 10346T) as the type strain] and Cupriavidus laharis sp. nov. [one strain, the type strain 1263aT (=CIP 108726T=PNCM 10347T)]. It is also suggested that Ralstonia sp. LMG 1197 (=JMP 134) should be included in the species C. pinatubonensis.

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Classification of Ralstonia pickettii-like isolates from the environment and clinical samples as Ralstonia insidiosa sp. nov.

Cited by 116 publications

References 29 publications

Diversity of intratunical bacteria in the tunic matrix of the colonial ascidian Diplosoma migrans

Diversity of intratunical bacteria in the tunic matrix of the colonial ascidian Diplosoma migrans

Wautersia gen. nov., a novel genus accommodating the phylogenetic lineage including Ralstonia eutropha and related species, and proposal of Ralstonia [Pseudomonas] syzygii (Roberts et al. 1990) comb. nov.

Cupriavidus pinatubonensis sp. nov. and Cupriavidus laharis sp. nov., novel hydrogen-oxidizing, facultatively chemolithotrophic bacteria isolated from volcanic mudflow deposits from Mt. Pinatubo in the Philippines

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