ABSTRACT. Visible and near-infrared (NIR) o ensure a wholesome and safe meat supply for consumers, the USDA Food Safety and Inspection Service (FSIS) has established policies for meat and poultry processing in order to minimize the risks of bacterial pathogens in meat and poultry products (USDA, 1994). More recently, FSIS implemented Pathogen Reduction, Hazard Analysis, and Critical Control Points (HACCP) programs, which hold processing plants accountable to detailed standard operating procedures developed by the plants to meet daily sanitation requirements (USDA, 1996). This is important to pathogen reduction because unsanitary practices in processing plants increase the likelihood of product cross contamination. Thus, plants must document daily records of completed sanitation standard Mention of a product or specific equipment does not constitute a guarantee or warranty by the U.S. Department of Agriculture and does not imply its approval to the exclusion of other products that may also be suitable.The operating procedures and are subject to hands-on sanitation verification by FSIS inspectors.Because poultry feces are the most likely source of pathogenic contamination in a poultry plant, FSIS inspectors use the established guidelines to identify fecal residues on hard surfaces in the processing environment, including equipment and tools. Evaluation and inspection of sanitation effectiveness is usually performed through one or more of the following methods: human visual inspection, microbiological culture analysis, bioluminescent ATP-based assays, and antibody-based microbiological tests. However, these labor-intensive and time-consuming procedures do not meet the needs of the poultry processing industry for an accurate, high speed, and non-invasive method that can provide near immediate results that are useful for monitoring the processing line in real-time. Thus, the USDA Agricultural Research Service has been developing hyperand multi-spectral reflectance and fluorescence imaging techniques for use in the detection of fecal material on chicken carcasses and on fresh fruit and vegetable products (Kim et al., 2003;Windham et al., 2003a;Park et al., 2005), and major developments have been achieved towards spectral imaging inspection systems for implementation on processing lines. The development of low-cost, reliable, and portable sensor systems is also being pursued, such as personal goggle and binocular devices (Ding et al., 2006), to provide affordable inspection tools to processors of all sizes. One key factor in successful applications is the use of a few essential spectral bands, which should not only reflect the chemical / physical information in the samples, but also maintain successive discrimination and T