Classification of some Linseed (Linum usitatissimum L.) Genotypes for Salinity Tolerance using Germination, Seedling Growth, and Ion Content

Abstract

Salinity reduces germination, delays emergence, and inhibits seedling growth of linseed (Linum usitatissimum L.) while some varieties are less affected by salinity than others. In this study, we aimed to determine the effects of NaCl levels (0, 5, 10, 20 and 30 dS m -1 ) on germination and seedling growth of 10 linseed genotypes (lines 87, 89, 104, 114, 193, 194, 209, 215, and to classify the genotypes for salinity tolerance using germination and seedling characteristics. Germination percentage, mean germination time, root and shoot length, seedling fresh and dry weight, Na + content and Na:K ratio of seedlings were investigated. Classification of linseed genotypes for salinity tolerance was done according to (i) combination of Principal Component and Cluster Analysis, (ii) Na + content, and (iii) Na:K ratio of seedling. The results showed that the highest values were obtained from lines 193, 194 and 215 except for mean germination time, while germination percentage was not adversely influenced by NaCl up to 20 dS m -1 . Seedling growth was inhibited at 20 dS m -1 although genotypes exhibited varying responses. Na + content was enhanced by NaCl, but seedling from lines 194, 193 and 215 had the lowest Na + content at all NaCl levels. Cluster analysis performed by multiple parameters revealed three groups for salinity tolerance. It was concluded that lines 193, 194, and 215 were tolerant, lines 87, 209, C-90, and cv. Sarı-85 were moderately tolerant and lines 89, 104, and 114 were salt-sensitive genotypes. Classification of genotypes for Na + content and Na:K ratio showed similar result for tolerant genotypes while different genotypes for sensitive group were detected.

Diversity of Linum genetic resources in global genebanks: from agro-morphological characterisation to novel genomic technologies – a review

“…Soil salinization significantly affects the growth and distribution of linseed (151). Only a few studies have reported screening of linseed germplasm against salinity-alkalinity stresses to identify salinitytolerant lines using germination, biomass and K + /Na + ratio (149,150,(180)(181)(182)(183), and the underlying biochemical and physiological mechanisms (151) (Table 2). Yu et al (184) reported differentially expressed genes (DEGs) and saline-alkaline tolerant miRNAs in flax (Lus-miRNAs) for the first time.…”

Molecular Advances to Combat Different Biotic and Abiotic Stresses in Linseed (Linum usitatissimum L.): A Comprehensive Review

“…According to Dubey et al [88], soil salinity-alkalinity in flax causes delayed germination and emergence, reduced seedling survival, erratic crop development, and decreased yield. In a few investigations, flax germplasm was tested for resistance to salinity-alkalinity stressors, and salinity-tolerant lines were found based on biomass, germination, seedling traits, and the K + /Na + ratio [92][93][94][95][96]. Wu et al [97] discovered genes in flax that increase the root length, improve membrane damage, and alter the ion distribution to confer salt tolerance.…”

Identification and Morphologic Characterization of Some Salt Resistant Exotic Safflower (<i>Carthamus Tinctorius</i> L.) Lines During Seedling Growth

“…Although safflower is considered a drought and salt tolerant plant species compared to other oilseed crops, yet its germination, emergence, and seedling development stages are highly sensitive to drought and salinity [13]. Crop cultivation in water-logged areas is seriously affected by the level of salinity in irrigation water quality that influences delayed emergence, low survival rate, irregular seedling establishment, and poor yield [14][15][16].…”