Bacterial infection of skin wounds, as one of the most important and potentially serious conditions (Appelgren et al., 2002) , is of increasing public health concern and causes an enormous medical and financial burden due to the rapid emergence of drug-resistant bacteria (Irwansyah et al., 2015; Mahmoudi and Serpooshan, 2012). Systemically and topically administered antibiotics do not effectively decrease the level of bacteria, due to resistance to antibiotics exhibited by some bacteria, such as multidrug-resistant Pseudomonas aeruginosa (MDRP) and methicillin-resistant Staphylococcus aureus (MRSA) , and formation of biofilms attached on various surfaces (Zhou et al., 2018). Water droplets in a high-velocity steam-air micromist jet spray (HVS-AMJS) (SGM-MX; Aqua Science Co., Yokohama, Japan) have a much stronger impact on a surface than droplets in air, since the kinetic energy of water droplets in steam is much higher than that of droplets in air (Fukuda et al., 2017). Furthermore, the generation and propagation of the shock wave upon impact of the droplets play a significant role in cleansing. Vapour steam cleaners or steam vapour systems are cleansing devices that use steam to quickly dry, cleanse, and sanitize inanimate surfaces (Fukuda et al., 2017). In this study, improved HVS-AMJS installed scattering prevention cover (Aqua Science Co., Yokohama, Japan) was evaluated as a prototype. Bacterial infection of skin wounds can be prevented by properly cleansing. It is expected that cleansing with disinfectants may be more effective than with water or saline. However, disinfectants used as substances in clinical settings, such as povidone-iodine and chlorhexidine gluconate have been demonstrated to be cytotoxic