Clearance Kinetics of Biomaterials Affects Stem Cell Retention and Therapeutic Efficacy

Lai, Chia-Yun; Wu, Pei‐Jhe; Roffler, Steve R.; Lee, Sho T.; Hwang, Shiaw Min; Wang, Shoei S.; Wang, Kuan; Hsieh, Patrick C.

doi:10.1021/bm401583b

Cited by 12 publications

(13 citation statements)

References 43 publications

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“…Therefore, in this study, HA hydrogel combined with CB‐MNC was used for the same approach after acute MI to investigate whether cell retention rate was improved. HA is a type of extracellular matrix with a rapid degradation rate (half‐life: 15–25 hours) [46]. Although HA degraded rapidly, we still found that the cell retention rate was increased approximately twofold in the CB‐MNC and HA combined group.…”

Section: Discussionmentioning

confidence: 72%

“…Although HA degraded rapidly, we still found that the cell retention rate was increased approximately twofold in the CB-MNC and HA combined group. Despite the short half-life of HA in vivo, it has been shown to benefit the injured heart by reducing scar formation and increasing cell survival and retention, neovascularization, and the efficacy of cell therapy in both rat and swine MI models [27,46,47]. In this study, we found that cell retention rate was increased approximately twofold in the CB-MNC and HA combined group.…”

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confidence: 57%

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Injection of Human Cord Blood Cells With Hyaluronan Improves Postinfarction Cardiac Repair in Pigs

Chang

Huang

Chen

et al. 2015

Stem Cells Translational Medicine

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This study using healthy human cord blood mononuclear cells (CB-MNCs) to treat myocardial infarction provides preclinical evidence that combined injection of hyaluronan and human CB-MNCs after myocardial infarction significantly increases cell retention in the peri-infarct area, improves cardiac performance, and prevents cardiac remodeling. Moreover, using healthy cells to replace dysfunctional autologous cells may constitute a better strategy to achieve heart repair and regeneration.

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Section: Discussionmentioning

confidence: 72%

mentioning

confidence: 57%

Injection of Human Cord Blood Cells With Hyaluronan Improves Postinfarction Cardiac Repair in Pigs

Chang

Huang

Chen

et al. 2015

Stem Cells Translational Medicine

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“…It would be important to be able to monitor changes in scaffold composition non-invasively over time, as stem cells are particularly sensitive to changes in environmental cues, including the physical microenvironment and mechanical stress [7–10]. Indeed, a recent imaging study using Alexa Fluor-labeled HA reported that the clearance kinetics of biomaterials affects stem cell retention and therapeutic efficacy [11]. However, optical imaging is limited to small animal studies and does not directly report on the molecular structure of the hydrogel.…”

Section: Introductionmentioning

confidence: 99%

Label-free imaging of gelatin-containing hydrogel scaffolds

et al. 2015

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Composite hyaluronic acid (HA) hydrogels containing gelatin are used in regenerative medicine as tissue-mimicking scaffolds for improving stem cell survival. Once implanted, it is assumed that these biomaterials disintegrate over time, but at present there is no non-invasive imaging technique available with which such degradation can be directly monitored in vivo. We show here the potential of chemical exchange saturation transfer magnetic resonance imaging (CEST MRI) as a label-free non-invasive imaging technique to monitor dynamic changes in scaffold composition in vivo. The CEST properties of the three individual hydrogel components (HA, gelatin-GelinS, and polyethylene glycol diacrylate) were first measured in vitro. The complete hydrogel was then injected into the brain of immunodeficient rag2−/− mice and CEST MR images were obtained at day 1 and 7 post-transplantation. In vitro, GelinS gave the strongest CEST signal at 3.6 ppm offset from the water peak, originating from the amide protons present in gelatin. In vivo, a significant decrease in CEST signal was observed at 1 week post-implantation. These results were consistent with the biodegradation of the GelinS component, as validated by fluorescent microscopy of implanted hydrogels containing Alexa Fluor 488-labeled GelinS. Our label-free imaging approach should be useful for further development of hydrogel formulations with improved composition and stability.

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“…In order to overcome these problems, cell therapy has been introduced using biocompatible and biodegradable biomaterials to reduce cell loss and long-term in vitro retention of stem cells. Currently in clinical trials, these biomaterials are widely used in drug and cell-delivery systems, regenerative medicine and tissue engineering in which to prevent the long-term survival of foreign substances in the body the release of cells are controlled (40).…”

Section: Application Of Mscs In Regenerative Medicine In Animal Modelsmentioning

confidence: 99%

Stem cell-based regenerative medicine

2019

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Definition and history of stem cell Stem cells are one of the main cells of the human body that have ability to grow more than 200 types of body cells (1). Stem cells, as non-specialized cells, can be transformed into highly specialized cells in the body (2). In the other words, Stem cells are undifferentiated cells with self-renewal potential, differentiation into several types of cells and excessive proliferation (3). In the past, it was believed that stem cells can only differentiate into mature cells of the same organ. Today, there are many evidences to show that stem cells can differentiate into the other types of cell as well as ectoderm, mesoderm and endoderm. The numbers of stem cells are different in the tissues such as bone marrow, liver, heart, kidney, and etc. (3,4). Over the past 20 years, much attention has been paid to stem cell biology. Therefore, there was a profound increase in the understanding of its characteristics and the therapeutic potential for its application (5). Today, the utilization of these cells in experimental research and cell therapy represents in such disorders including hematological, skin regeneration and heart disease in both human and veterinary medicine (6).The history of stem cells dates back to the 1960s, when Friedenstein and colleagues isolated, cultured and differentiated to osteogenic cell lineage of bone marrow-derived cells from guinea pigs (7). This project created a new perspective on stem cell research. In the following, other researchers discovered that the bone marrow contains fibroblastlike cells with congenic potential in vitro, which were

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Clearance Kinetics of Biomaterials Affects Stem Cell Retention and Therapeutic Efficacy

Cited by 12 publications

References 43 publications

Injection of Human Cord Blood Cells With Hyaluronan Improves Postinfarction Cardiac Repair in Pigs

Injection of Human Cord Blood Cells With Hyaluronan Improves Postinfarction Cardiac Repair in Pigs

Label-free imaging of gelatin-containing hydrogel scaffolds

Stem cell-based regenerative medicine

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