Cleavable Molecular Beacon for Hg<sup>2+</sup> Detection Based on Phosphorothioate RNA Modifications

Huang, Po‐Jung Jimmy; Wang, Feng; Liu, Juewen

doi:10.1021/acs.analchem.5b01362

Cited by 68 publications

(44 citation statements)

References 34 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The reason that more than one PS-RNA linkage might be needed is due to the low cleavage yield. Once Hg 2+ is added, only ~16% of each PS-RNA linkage undergoes cleavage, 24 while the rest are desulfurized to the normal 7 phosphate backbone and cannot be further cleaved by Hg 2+ . 34 To optimize the design, we tested DNAs with 1, 3, and 5 PS-RNA linkages (Probe 1-3).…”

Section: Resultsmentioning

confidence: 99%

“…36 Our previous assays indicated that Hg 2+ can still cleave the PS RNA even in the presence of other metal ions. 24 Overall, using the NGO quencher did not alter the metal interaction trend in this sensing mechanism. Sensor selectivity at a few metal concentrations.…”

mentioning

confidence: 84%

“…24 Hg 2+ efficiently cleaves the PS-RNA linkage due to its extremely strong thiophilicity. This method has excellent specificity for Hg 2+ and the reaction proceeds very quickly.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Hg²⁺ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA

Huang

Ballegooie

Liu

2016

Analyst

Self Cite

View full text Add to dashboard Cite

Mercury is a highly toxic heavy metal and many DNA-based biosensors have been recently developed for Hg 2+ detection in water. Among them, thymine-rich DNA is the most commonly used for designing Hg 2+ sensors. However, the thymine-Hg 2+ interaction is strongly affected by buffer conditions. We recently reported a molecular beacon containing phosphorothioate (PS)-modified RNA linkages that can be cleaved by Hg 2+ . In this work, the fluorescence quenching and DNA adsorption properties of nano-sized graphene oxide (NGO) were used to develop a new sensor using the PS-RNA chemistry. Three DNA probes were tested, containing one, three and five PS-RNA linkages, respectively. Finally, a fluorophore-labeled poly-A DNA with five PS-RNA linkages was selected and adsorbed by NGO. In the presence of Hg 2+ , the fluorophore was release from NGO due to the cleavage reaction, resulting in fluorescence enhancement. This sensor is highly selective for Hg 2+ with a detection limit of 8.5 nM Hg 2+ . For comparison, a fluorophore-labeled poly-T DNA was also tested, which responded to Hg 2+ slower and was inhibited by high NaCl concentrations, while the PS-RNA probe was more tolerable to different buffer conditions. This work indicates a new way of interfacing DNA with NGO for Hg 2+ detection.3

show abstract

Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 84%

See 1 more Smart Citation

Hg²⁺ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA

Huang

Ballegooie

Liu

2016

Analyst

Self Cite

View full text Add to dashboard Cite

show abstract

“…46,47 Since the Hg 2+ cleavage is finished almost instantaneously, this kinetic signature can be used to distinguish Hg 2+ from Cu 2+ (vide infra). Overall, it appears that PSCu10 is the best candidate in terms of sensitivity and selectivity for Cu 2+ detection and it was chosen for further studies.…”

Section: Figure 1 (A)mentioning

confidence: 99%

An Ultrasensitive Light-up Cu²⁺ Biosensor Using a New DNAzyme Cleaving a Phosphorothioate-Modified Substrate

2016

Self Cite

View full text Add to dashboard Cite

Cu 2+ is a very important metal ion in biology, environmental science, and industry. Developing biosensors for Cu 2+ is a key topic in analytical chemistry. DNAzyme-based sensors are highly attractive for their excellent sensitivity, stability, and programmability. In the past decade, a few Cu 2+ biosensors were reported using DNAzymes with DNA cleavage or DNA ligation activity.However, they require unstable ascorbate or imidazole activation. So far, no RNA-cleaving DNAzymes specific for Cu 2+ are known. In this work, a phosphorothioate (PS) RNA containing library was used for in vitro selection, and a few new Cu 2+ -specific RNA-cleaving DNAzymes were isolated. Among them, a DNAzyme named PSCu10 was studied further. It has only eight nucleotides in the enzyme loop with a cleavage rate of 0.1 min -1 in the presence of 1 µM Cu 2+ at pH 6.0 (its optimal pH). Between the two diastereomers of the PS RNA chiral center, the Rp isomer is 37 times more active than the Sp one. Among the other divalent metal ions, only Hg 2+ can cleave the substrate due to its extremely high thiophilicity. A catalytic beacon sensor was designed with a detection limit of 1.6 nM Cu 2+ and extremely high selectivity. PSCu10 is specific for Cu 2+ and it has no cleavage in the presence of ascorbate, which reduces Cu 2+ to Cu + .3

show abstract

“…The effect of pH on this reaction was also studied, and Hg 2+ achieved more cleavage at lower pH ( Figure S1), which is consistent with our previous study. 50 While Tl 3+ can cleave this DNA, it is not the only metal capable of performing this reaction. It results in a low cleavage yield.…”

Section: 38 39mentioning

confidence: 99%

Desulfurization Activated Phosphorothioate DNAzyme for the Detection of Thallium

2015

Self Cite

View full text Add to dashboard Cite

show abstract

Cleavable Molecular Beacon for Hg²⁺ Detection Based on Phosphorothioate RNA Modifications

Abstract: Mercury is a highly toxic heavy metal and detection of Hg 2+

Cited by 68 publications

References 34 publications

Hg²⁺ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA

Hg²⁺ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA

An Ultrasensitive Light-up Cu²⁺ Biosensor Using a New DNAzyme Cleaving a Phosphorothioate-Modified Substrate

Desulfurization Activated Phosphorothioate DNAzyme for the Detection of Thallium

Contact Info

Product

Resources

About

Cleavable Molecular Beacon for Hg2+ Detection Based on Phosphorothioate RNA Modifications

Abstract: Mercury is a highly toxic heavy metal and detection of Hg 2+

Cited by 68 publications

References 34 publications

Hg2+ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA

Hg2+ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA

An Ultrasensitive Light-up Cu2+ Biosensor Using a New DNAzyme Cleaving a Phosphorothioate-Modified Substrate

Desulfurization Activated Phosphorothioate DNAzyme for the Detection of Thallium

Contact Info

Product

Resources

About

Cleavable Molecular Beacon for Hg²⁺ Detection Based on Phosphorothioate RNA Modifications

Hg²⁺ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA

Hg²⁺ detection using a phosphorothioate RNA probe adsorbed on graphene oxide and a comparison with thymine-rich DNA

An Ultrasensitive Light-up Cu²⁺ Biosensor Using a New DNAzyme Cleaving a Phosphorothioate-Modified Substrate