Clerodane diterpenoids from the Chinese liverwort Jamesoniella autumnalis and their anti-inflammatory activity

Hong

et al. 2021

Plants

Bryophytes contain a variety of bioactive metabolites, but studies about the anti-inflammatory effect of bryophytes are meager. Therefore, the present study aimed to compare the anti-inflammatory effect of methanol extract of Marchantia polymorpha L. (liverwort) and Racomitrium canescens (Racomitrium moss) in lipopolysaccharide (LPS)-induced HaCaT cells. To evaluate the anti-inflammatory effect of liverwort and Racomitrium moss, the levels of nitric oxide (NO) production and the mRNA expression of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2) and tumor necrosis factor-α (TNF-α), and interleukin (IL)-6 and IL-1β in LPS-induced HaCaT cells were measured. The methanol extract of liverwort and Racomitrium moss significantly decreased LPS-induced NO production in HaCaT cells. When compared with Racomitrium moss extract, pre-treatment with methanol extract of liverwort markedly inhibited the expression of iNOS, COX-2, IL-6, and IL-1β at the concentration of 100 µg/mL with the exception of TNF-α. Further, liverwort extract markedly attenuated the production of TNF-α, IL-6, and IL-1β in the culture medium. In addition, ethyl acetate and butanol fractions obtained from the methanol extract of liverwort showed remarkable inhibitory activity against the production of NO in LPS-stimulated HaCaT cells. The LC-MS data revealed the presence of bisbibenzyl types of bioactive components in the methanol extract of liverwort. These data demonstrate that liverwort extract exhibits effective inhibitory activity against the production of inflammatory mediators in LPS-induced HaCaT cells and may be useful for the treatment of inflammation-mediated diseases.

Section: Resultsmentioning

confidence: 99%

Anti-Inflammatory Effect of Liverwort (Marchantia polymorpha L.) and Racomitrium Moss (Racomitrium canescens (Hedw.) Brid.) Growing in Korea

Hong

et al. 2021

Plants

“…Metabolites such as marchantin A, plagochilin E, and riccardin C exhibit cytotoxic activities against chemoresistant prostate cancer [3,100]. In addition to the aforementioned active compounds, those with antioxidant [135][136][137], anti-inflammatory [138][139][140], psychoactive [111], antiviral [141], muscle-relaxing [142], neuroprotective [142,143], anti-HIV [144], and insecticidal activities can be distinguished [145,146]. Compounds with nematicidal activity have potential in agricultural industry as natural plant-protection products.…”

Section: Discussionmentioning

confidence: 99%

Modern Use of Bryophytes as a Source of Secondary Metabolites

et al. 2022

Bryophytes constitute a heterogeneous group of plants which includes three clades: approximately 14,000 species of mosses (Bryophyta), 6000 species of liverworts (Marchantiophyta), and 300 species of hornworts (Anthocerotophyta). They are common in almost all ecosystems, where they play important roles. Bryophytes lack developed physical barriers, yet they are rarely attacked by herbivores or pathogens. Instead, they have acquired the ability to produce a wide range of secondary metabolites with diverse functions, such as phytotoxic, antibacterial, antifungal, insect antifeedant, and molluscicidal activities. Secondary metabolites in bryophytes can also be involved in stress tolerance, i.e., in UV-absorptive and drought- and freezing-tolerant activities. Due to these properties, for centuries bryophytes have been used to combat health problems in many cultures on different continents. Currently, scientists are discovering new, unique compounds in bryophytes with potential for practical use, which, in the age of drug resistance, may be of considerable importance. The aim of this review is to present bryophytes as a potential source of compounds with miscellaneous possible uses, with a focus on volatile compounds and antibacterial, antifungal, and cytotoxic potential, and as sources of materials for further promising research. The paper also briefly refers to the methods of compound extraction and acquisition. Formulas of compounds were drawn by the authors using ChemDraw software (PerkinElmer, Boston, MA, USA) with reference to data published in various papers, the ACD/Labs dictionary database, PubChem, and Scopus. The data were gathered in February 2022.

Chemistry & Biodiversity

“…The inhibitory potency of the undescribed sesquiterpenoids on LPS-induced NO production in RAW 264.7 cells was implemented by the Griess reagent and MTT methods. [25] 9.0 × 10 4 cells/mL RAW 264.7 cells and 1.0 μg/mL LPS were incubated in 96-well plates in DMEM. Thereafter, a series of sesquiterpenoids (3.125, 6.25, 12.5, 25.0, 50.0, and 100.0 μM) were added and incubated for 24 h. The positive control group was added with didox (100.0 μM).…”

Section: Anti-inflammatory Activity Testsmentioning

confidence: 99%

Unprecedented 4,9‐Seco‐Oplopanane and Seven Drimane Sesquiterpenoids from the Chinese Liverwort Lejeunea flava (Sw.) Nees

Lou

Zhu

et al. 2022

Self Cite

An unprecedented 4,9-seco-oplopanane (1), two undescribed drimane epimers (2 and 3), and five known drimane sesquiterpenoids (4 -8) were isolated from the Chinese liverwort Lejeunea flava (Sw.) Nees. The structures of the new sesquiterpenoids were determined using nuclear magnetic resonance spectroscopy, electronic circular dichroism calculations, and single-crystal X-ray diffraction measurements. The inhibitory capacity of the new compounds against nitric oxide production in lipopolysaccharide-induced RAW 264.7 murine macrophages, along with the cytotoxicity of the new compounds against A549 and HepG-2 human cancer cell lines, were discussed.