‘Click chemistry’ for diagnosis: a patent review on exploitation of its emerging trends

Abstract

In recent years, considerable development in the click concept has encouraged researchers in using click reactions in almost every branch of industry that uses chemistry. Click chemistry for chemical ligation has been immensely explored in the field of biotechnology especially for detection, diagnosis and therapeutics.

Recent trends in click chemistry as a promising technology for virus-related research

“…It also provides platforms for viral detection, drug development and targeted delivery. To date, more products generated via click chemistry are likely to become commercially available in the field of biotechnology, especially for visualization, drug discovery, diagnosis, and therapeutics (Mandhare et al, 2014;Xu and Jones, 2015). Efforts are still needed to resolve the limitations of click chemistry in live cells and animals/humans, especially the cytotoxic activities of the compounds generated from click reactions.…”

Measuring and Suppressing the Oxidative Damage to DNA During Cu(I)-Catalyzed Azide–Alkyne Cycloaddition

“…10 However, because of their relatively low sensitivity, these methods cannot detect very low levels of damage that, while sporadic, are detrimental for most in vivo chemical biology applications. Such damage may also be a concern for a number of methods that use click chemistry to prepare DNA bioconjugates 30 for sensing, 31,32 diagnostics, 33 sequencing, 34 and gene synthesis. [35][36][37] Therefore, owing to the biological and technological importance of maintaining genomic integrity, a highly sensitive method for directly measuring the damage to DNA would be valuable for improving the biocompatibility of CuAAC chemistry.…”

“…By combining high-throughput and direct quantification of DNA damage with measurement of coupling kinetics, our approach will broaden the utility of CuAAC for bioconjugation in both solution phase and on solid surfaces. 51,55 For example, CuAAC bioconjugation has been utilized in a number of sensing and diagnostic applications, [31][32][33]53 and the results presented here may lead to increased specificity by reducing the extent of unintended modification of the probe molecules. Because the qPCR-based assay is also applicable to live cells and organisms, 38,39 our approach to optimizing CuAAC, which is informed by quantitative information concerning DNA damage, may help to improve the biocompatibility of CuAAC for in vivo applications.…”