Isotopic (d 13 C, d 18 O) and elemental (Mg, Sr, Mn, Fe) compositions were analysed in sclerochronological profiles of several shells of late Cretaceous rudist bivalves from Greece, Turkey, Somalia and the Arabian Peninsula. The preservation of original compositions of low-Mg calcite of outer shell layers is indicated by constant and high Sr, generally low Fe and Mn, and the preservation of fibrous-prismatic ultrastructures. Cyclic variations in d 18 O and Mg are interpreted to reflect seasonal temperature/salinity cycles and, thus, annual growth increments. In shells of Torreites, amplitudes of correlated d 13 C and d 18 O cycles cannot be related to reasonable palaeotemperatures or salinity. This isotopic pattern reflects vital fractionations of an extent which is unknown from modern bivalves. In contrast, almost identical ranges and amplitudes of d 18 O cycles are observed in 13 shells of five species from Santonian-Campanian localities in central Greece and northern Turkey, suggesting that seasonal variations in environmental conditions were recorded without significant vital fractionations. The effect of seasonal salinity changes on d 18 O of the shells is evaluated, and mean palaeotemperatures are constrained within the range of 30-32.5 7C. The annual range of temperature was estimated to be 7 7C, assuming a constant salinity. This agrees with other isotopic proxies of Late Cretaceous palaeotemperatures, and with global circulation models which predict higher low-latitude sea-surface temperatures than the present ones.