The Paris Agreement aims for a long-term temperature target of holding global temperature increase well below 2°C and pursuing efforts to keep warming to no more than 1.5°C (United Nations/Framework Convention on Climate Change, 2015). However, there is a substantial gap between the required mitigation efforts to achieve this ambitious target and planned national policies toward emissions reductions. According to the Change (IPCC) Special Report "Global warming of 1.5°C," achieving the temperature target without an overshoot, that is, temporarily exceeding the 1.5°C or 2°C limits, requires a rapid decline in global net Abstract There is a substantial gap between the current emissions of greenhouse gases and levels required for achieving the 2°C and 1.5°C temperature targets of the Paris Agreement. Understanding the implications of a temperature overshoot is thus an increasingly relevant research topic. Here we explore the carbon cycle feedbacks over land and ocean in the SSP5-3.4-OS overshoot scenario by using an ensemble of Coupled Model Intercomparison Project 6 Earth system models. Models show that after the CO 2 concentration and air temperature peaks, land and ocean are decreasing carbon sinks from the 2,040s and become sources for a limited time in the 22nd century. The decrease in the carbon uptake precedes the CO 2 concentration peak. The early peak of ocean uptake stems from its dependency on the atmospheric CO 2 growth rate. The early peak of the land uptake occurs due to a larger increase in ecosystem respiration than the increase in gross primary production, as well as due to a concomitant increase in land-use change emissions primarily attributed to the wide implementation of biofuel croplands. The carbon cycle feedback parameters amplify after the CO 2 concentration and temperature peaks due to inertia of the Earth system so that land and ocean absorb more carbon per unit change in the atmospheric CO 2 change (stronger negative feedback) and lose more carbon per unit temperature change (stronger positive feedback) compared to if the feedbacks stayed unchanged. The increased negative CO 2 feedback outperforms the increased positive climate feedback. This feature should be investigated under other scenarios.Plain Language Summary A large gap between required and currently planned greenhouse gas emission reductions makes possible overshooting the 2°C target of the Paris Agreement before the temperature can return below the target levels. We explore the response of the global carbon cycle to overshoot by analyzing the simulations of state-of-art models under an overshoot pathway, where the emissions increase until the 2,030s and exhibit a steep reduction thereafter. The land and ocean continue to take up carbon from the atmosphere throughout the 21st century, albeit at a reduced rate. The decrease in the ocean carbon uptake occurs before the CO 2 concentration peak due to its dependence on the rate of the atmospheric CO 2 change, and the decrease in the land uptake occurs due to a stronger increase in t...