Clindamycin-resistant<i>Streptococcus pneumoniae</i>

Montagnani, Francesca; Zanchi, Alessandra; Stolzuoli, Lucia; Croci, Leonardo; Cellesi, C

doi:10.3201/eid1305.060699

Cited by 10 publications

(7 citation statements)

References 7 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Finally, we also found one G. adiacens isolate that was clindamycin resistant but susceptible to erythromycin, a phenotype that has been infrequently described in Streptococcus spp. (40)(41)(42).…”

Section: Discussionmentioning

confidence: 99%

Antimicrobial Susceptibilities of Abiotrophia defectiva, Granulicatella adiacens, and Granulicatella elegans

Alberti

Hindler

Humphries

2016

Antimicrob Agents Chemother

View full text Add to dashboard Cite

Nutritionally variant streptococci (NVS) are fastidious Gram-positive cocci comprised of the species Abiotrophia defectiva, Granulicatella adiacens, and Granulicatella elegans. NVS are an important cause of bacteremia and infective endocarditis (IE) associated with significant morbidity and mortality. Antimicrobial susceptibility testing (AST) was performed for 14 antimicrobials using the broth microdilution MIC method described in the Clinical and Laboratory Standards Institute (CLSI) M45 guideline. A total of 132 clinical NVS blood isolates collected from 2008 to 2014 were tested. Species level identification of NVS isolates was achieved by 16S rRNA gene sequencing and/or matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS). Ninety isolates were identified as G. adiacens, 37 as A. defectiva, and 5 as G. elegans. All isolates were susceptible to vancomycin (MIC 90 ‫؍‬ 1 g/ml), and none displayed high-level resistance to aminoglycosides. G. adiacens was considerably more susceptible to penicillin than A. defectiva (38.9% versus 10.8% of isolates susceptible) but was less susceptible to cephalosporins than was A. defectiva (43.3% versus 100% of isolates susceptible to ceftriaxone). Several isolates were resistant to levofloxacin (6%), erythromycin (51%), and clindamycin (10%). The MIC 90 for daptomycin was >4 g/ml for G. adiacens and A. defectiva. G. elegans isolates were 100% susceptible to all antimicrobials tested, with the exception of erythromycin, to which only 20% were susceptible. This study provides antimicrobial susceptibility data for a recent collection of NVS and demonstrates important NVS species-related differences with respect to susceptibility to penicillin, cephalosporins, carbapenems, and daptomycin. Species-level identification of NVS organisms when susceptibility testing is not readily available may aid in treatment decisions.

show abstract

Section: Discussionmentioning

confidence: 99%

Antimicrobial Susceptibilities of Abiotrophia defectiva, Granulicatella adiacens, and Granulicatella elegans

Alberti

Hindler

Humphries

2016

Antimicrob Agents Chemother

View full text Add to dashboard Cite

show abstract

“…To understand the resistance mechanism for clindamycin, the efflux pump inhibitor reserpine (Alfa Aesar) was dissolved in DMSO and added to the setup for microdilution testing at a final concentration of 10 mg ml 21 as described before (Montagnani et al, 2007). As the MIC was not influenced, an efflux mechanism is unlikely.…”

mentioning

confidence: 99%

“…(2), penicillin (0.25), rifampicin (0.25), streptomycin (8-16), tetracycline (1-2), trimethoprim (0.063-0.125) and vancomycin (0.5-1.0), but were resistant to nalidixic acid (.256), which is typical for the genus Listeria, and additionally to clindamycin (32). To understand the resistance mechanism for clindamycin, the efflux pump inhibitor reserpine (Alfa Aesar) was dissolved in DMSO and added to the setup for microdilution testing at a final concentration of 10 mg ml 21 as described before (Montagnani et al, 2007). As the MIC was not influenced, an efflux mechanism is unlikely.…”

mentioning

confidence: 99%

Listeria fleischmannii sp. nov., isolated from cheese

Bertsch

Rau

Eugster

et al. 2013

International Journal of Systematic and Evolutionary Microbiology

View full text Add to dashboard Cite

A study was performed on three isolates (LU2006-1T, LU2006-2 and LU2006-3), which were sampled independently from cheese in western Switzerland in 2006, as well as a fourth isolate (A11-3426), which was detected in 2011, using a polyphasic approach. The isolates could all be assigned to the genus Listeria but not to any known species. Phenotypic and chemotaxonomic data were compatible with the genus Listeria and phylogenetic analysis based on 16S rRNA gene sequences confirmed that the closest relationships were with members of this genus. However, DNA–DNA hybridization demonstrated that the isolates did not belong to any currently described species. Cell-wall-binding domains of Listeria monocytogenes bacteriophage endolysins were able to attach to the isolates, confirming their tight relatedness to the genus Listeria . Although PCR targeting the central portion of the flagellin gene flaA was positive, motility was not observed. The four isolates could not be discriminated by Fourier transform infrared spectroscopy or pulsed-field gel electrophoresis. This suggests that they represent a single species, which seems to be adapted to the environment in a cheese-ripening cellar as it was re-isolated from the same type of Swiss cheese after more than 5 years. Conjugation experiments demonstrated that the isolates harbour a transferable resistance to clindamycin. The isolates did not exhibit haemolysis or show any indication of human pathogenicity or virulence. The four isolates are affiliated with the genus Listeria but can be differentiated from all described members of the genus Listeria and therefore they merit being classified as representatives of a novel species, for which we propose the name Listeria fleischmannii sp. nov.; the type strain is LU2006-1T ( = DSM 24998T = LMG 26584T).

show abstract

“…This resistance phenotype has been associated previously with a lincosamide‐inactivating nucleotidyltransferase encoded by the linB gene in Enterococcus faecium [7]; however, the linB gene was not detected in any of these six isolates. Although this resistance phenotype has been reported in other streptococci [10,15–17], it has not been described previously in GCS [18,19]. These six isolates were negative according to PCR for all the resistance genes investigated.…”

Section: Erythromycin Resistance Phenotypes and Genotypes Among Clinmentioning

confidence: 63%

Prevalence and mechanisms of erythromycin and clindamycin resistance in clinical isolates of β-haemolytic streptococci of Lancefield groups A, B, C and G in Seville, Spain

Diaz

Sanchez

Martín

2008

Clinical Microbiology and Infection

View full text Add to dashboard Cite

Susceptibility to erythromycin and clindamycin was determined in 860 consecutive clinical isolates of beta-haemolytic streptococci belonging to groups A (GAS, n = 134), B (GBS, n = 689), C (GCS, n = 19) and G (GGS, n = 18). Erythromycin resistance was 26.1% in GAS, 15.7% in GBS, 5.3% in GCS and 33.3% in GGS. The highest rate of clindamycin resistance (33.3%) was in GGS, followed by GBS (15.8%), GCS (15.8%) and GAS (5.2%). The M phenotype was predominant in GAS (80%), the constitutive MLS(B) phenotype was predominant in GBS (75%), and all GGS isolates showed the inducible MLS(B) phenotype. The uncommon erythromycin-susceptible and clindamycin-resistant phenotype was found in four GBS and two GCS isolates.

show abstract

Clindamycin-resistantStreptococcus pneumoniae

Cited by 10 publications

References 7 publications

Antimicrobial Susceptibilities of Abiotrophia defectiva, Granulicatella adiacens, and Granulicatella elegans

Antimicrobial Susceptibilities of Abiotrophia defectiva, Granulicatella adiacens, and Granulicatella elegans

Listeria fleischmannii sp. nov., isolated from cheese

Prevalence and mechanisms of erythromycin and clindamycin resistance in clinical isolates of β-haemolytic streptococci of Lancefield groups A, B, C and G in Seville, Spain

Contact Info

Product

Resources

About