Clinical and Bacteriological Profiles of Blood Culture Positive Sepsis in Newborns

Abstract

Neonatal infections currently cause about 1.6 million deaths annually in developing countries 1 . Sepsis and meningitis is responsible for most of these deaths. This study was undertaken to determine the clinical presentations, bacteriological profiles and antibiotic sensitivity patterns of isolates from blood cultures of neonates admitted in a tertiary care hospital in Eastern Nepal. All blood culture reports (n=103) during January 2006 -February 2007 from newborns admitted in neonatal division at BP Koirala Institute of Health Sciences, Nepal were analyzed and antibiotic sensitivity patterns were studied. The positive blood culture was 20% (103/513). Most (97.1%) of the sepsis was caused by single organism, while polymicrobial aetiology was observed in 2.9% cases. Meningitis was documented in 9(8.7%) cases. Staphylococcus aureus (38.8%) and coagulase negative staphylococcus (CONS) (21.3%) are the commonest isolates in blood culture. Among gram-negative organisms, Klebsiella species (11.6%) and Enterobacter species (9.7%) were the leading cause of neonatal sepsis. Majority of newborns with neonatal sepsis presented with refusal to feeds (42.7%), fever (41.7%) and jaundice (41.7%). Most of the organisms showed sensitivity with amino glycosides (gentamicin and amikacin) and third generation cephalosporins. It is concluded that Staphylococcus aureus, CONS, and Klebsiella species remain the principal organisms causing neonatal sepsis and first line antibiotics like amino glycosides should be first choice of drugs.

Bacteriological profile and antibiotic sensitivity patterns of blood cultures

“…Our results correlates from the study by G. Karthikeyan et al who reported an equal proportion of EOS (50%) and LOS (50%) cases in their study, while the study by R S Jaswal et al (74%) and Shrestha P et al (66.9 %) showed a higher proportion of LOS cases. [14][15][16] Our results differ with higher percentage of EOS was seen in the studies done by Tallur et al (83.47%) and Roy et al (71.30%). 4,9 This could be due to ascending infection following rupture of membranes or during the passage of baby through the infected birth canal or at the.…”

Comparison of the Lytic Blood Culture Method with the Conventional Blood Culture Method in Cases of Enteric Fever in a Tertiary Care Hospital

“…Culture positivity by conventional blood culture method was 13.3% in this study. A study from Chandigarh has reported overall blood culture positivity of 9.94% 18 which is less than this study. Culture positivity of enteric fever by the lytic centrifugation method was 17.3 % in this study.…”

Blood Stream Infection in Pediatric Patients of a Tertiary Care Hospital: A Bacteriological and Antimicrobial Profile

“…The spectrum of causative organisms changes over times and varies from region to region. This is due to the changing pattern of antibiotic use and changes in lifestyle (Shrestha et al, 2008).…”