Clinical and genetic characteristics of unusual G12P[11] rotavirus strains recovered from neonates: A study from Pune, Western India

Ranshing, Sujata S.; Bavdekar, Ashish; Vaidya, Umesh; Behera, Manas Kumar; Walimbe, Atul M.; Gopalkrishna, Varanasi

doi:10.1016/j.meegid.2019.02.014

Cited by 9 publications

(10 citation statements)

References 29 publications

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“…Western India, where we reported a high endemic prevalence of unique G12P [11] strain causing asymptomatic infections among preterm neonates. 19 Thus, this study describes sharp disparity to general assumption that rotaviruses cause asymptomatic infection in neonates.…”

Section: Discussionmentioning

confidence: 78%

“…15,16,18,19 In our previous study carried out during 2016-2018 to determine the prevalence of RV among neonates admitted at NICUs in Pune, western India, unusual human-bovine-like RV strain; G12P [11] was found as the most predominant type and 98.68% of the infected neonates were asymptomatic. 19 The full genome-based sequence analysis of the asymptomatic G12P [11] strain revealed multiple-gene reassortment events, containing 116E-like VP4 gene, VP7 gene from G12 strains and VP3 gene of porcine ancestry in a human Wa-like backbone. 20 RVI in neonates are usually asymptomatic though there have been infrequent reports available on nosocomial outbreaks of clinical disease in this population.…”

mentioning

confidence: 95%

“…17 So far, RV strains bearing P [11] specificity in combinations with G3, G4, G9, G10, and G12 have been detected in neonates in India. 15,16,18,19 In our previous study carried out during 2016-2018 to determine the prevalence of RV among neonates admitted at NICUs in Pune, western India, unusual human-bovine-like RV strain; G12P [11] was found as the most predominant type and 98.68% of the infected neonates were asymptomatic. 19 The full genome-based sequence analysis of the asymptomatic G12P [11] strain revealed multiple-gene reassortment events, containing 116E-like VP4 gene, VP7 gene from G12 strains and VP3 gene of porcine ancestry in a human Wa-like backbone.…”

mentioning

confidence: 95%

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Complete genomic analysis of uncommon G12P[11] rotavirus causing a nosocomial outbreak of acute diarrhea in the newborns in New Delhi, India

Ranshing

Ganorkar

Ramji

et al. 2021

Journal of Medical Virology

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Rotaviruses (RVs) are the major causative agents of acute gastroenteritis in children, but in neonates, RV infections are generally nosocomial in origin and mostly asymptomatic. However, there have been infrequent reports of nosocomial outbreaks of clinical disease in this population. In this study, we describe uncommon RV genotype; G12P [11] associated with an outbreak of acute gastroenteritis in the neonatal ward and neonatal intensive care unit (NICU) in New Delhi, North India.Full-genome analyses of the pathogenic G12P [11] strain was carried out to map the genotype constellation and further to explore the variations in the antigenic epitopes on the immunodominant VP7 and VP4 proteins, the amino acid sequences were compared with neonatal strains; ROTAVAC ® (G9P [11]) and asymptomatic G12P [11] and also other G/P-type matched strains. The study revealed G12-P[11]-I1-R1-C1-M1-A1-N1-T1-E1-H1 human Wa-like genotype constellation and highlights evidence of gene reassortment. No significant differences were observed in the sequences of structural (except VP3) and nonstructural encoding genes of G12P [11] strains recovered from symptomatic and asymptomatic neonates.Presence of additional N-linked glycosylation site was noted in the G12 strains, as a consequence of a change from Asp→Asn at amino acid position 238. Interestingly, only two and four amino acids substitution within the 7-1a and 8-1 antigenic epitope were observed, respectively, compared with asymptomatic G12P [11] strain. The study emphasizes the importance of close monitoring of RV outbreaks in neonates for early alarming of novel strain.

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Section: Discussionmentioning

confidence: 78%

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confidence: 95%

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confidence: 95%

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Complete genomic analysis of uncommon G12P[11] rotavirus causing a nosocomial outbreak of acute diarrhea in the newborns in New Delhi, India

Ranshing

Ganorkar

Ramji

et al. 2021

Journal of Medical Virology

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“…The frequency of G12P[11] notably increased from 35.9 to 56.8% between 2017 and 2019 making it the predominant circulating genotype in the neonatal ICU and nursery. The emergence of G12P[11] among neonates has been previously reported in Pune and found to be 24.3% 20 . In 2014, G12P[11] first emerged in Kolkata, India in children under 5 years 21 .…”

Section: Discussionmentioning

confidence: 99%

“…Phylogenetic analysis of G12 strains shows 88.5% nucleotide identity with prototype strain L26 in lineage I which was first reported in the Philippines in 1987. The G12 genotype origin is unknown, although pigs are believed to be its primary host 20 . Majority of the study strains belonged to lineage IV with P[9], P[8], and P[11] types.…”

Section: Discussionmentioning

confidence: 99%

A shift in circulating rotaviral genotypes among hospitalized neonates

Reju

Srikanth

Selvarajan

et al. 2022

Sci Rep

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In neonates, rotavirus (RV) infection is generally nosocomial. The control of rotaviral infection within hospital settings is challenging due to prolonged shedding of the virus and contamination of the surrounding environment. There are few studies that have reported asymptomatic infection within neonates. In this study, neonates were screened for RV infection and possible clinical manifestations that may play a role in RV acquisition were analysed. Stool samples were collected from 523 hospitalized neonates admitted for > 48 h in a low-cost and higher-cost tertiary centre. RV antigen was screened using ELISA and the samples which tested positive were confirmed by semi-nested RT-PCR. RV was detected in 34% of participants and genotypes identified included G12P[11] (44.4%), G10 P[11] (42.6%), G10G12P[11] (10.1%) and G3P[8] (2.9%). ICU admissions were associated with higher viral shedding (p < 0.05). Hospitalization in the low-cost facility ICU was associated with higher RV acquisition risk (p < 0.05). RV was detected in higher rates (36.9%) among neonates with gastrointestinal manifestations. G10P[11] was the predominant genotype for several years (1988–2016) among neonates within India. The preponderance of an emerging G12P[11] genotype and heterotypic distribution was documented. RV surveillance is important to identify emerging strains and establish the road ahead in managing RV infection.

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Identification of rotavirus A strains in small ruminants: first detection of G8P[1] genotypes in sheep in Turkey

Timurkan

Alkan

2019

Arch Virol

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Clinical and genetic characteristics of unusual G12P[11] rotavirus strains recovered from neonates: A study from Pune, Western India

Cited by 9 publications

References 29 publications

Complete genomic analysis of uncommon G12P[11] rotavirus causing a nosocomial outbreak of acute diarrhea in the newborns in New Delhi, India

Complete genomic analysis of uncommon G12P[11] rotavirus causing a nosocomial outbreak of acute diarrhea in the newborns in New Delhi, India

A shift in circulating rotaviral genotypes among hospitalized neonates

Identification of rotavirus A strains in small ruminants: first detection of G8P[1] genotypes in sheep in Turkey

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