Clinical and immunologic variables in skin of patients with atopic eczema and either positive or negative atopy patch test reactions

Langeveld-Wildschut, Elisabeth G.; Bruijnzeel, P. L. B.; Mudde, Geert C.; Versluisa, Coraline; Dijka, Adrie G. Van Ieperen-Van; Biharia, Ilse C.; Knol, Edward F.; Thepen, Theo; Bruijnzeel-Koomen, Carla A.F.M.; Reijsen, Frank C. van

doi:10.1067/mai.2000.106544

Cited by 88 publications

(67 citation statements)

References 30 publications

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“…The atopy patch test, i.e., the epicutaneous application of relevant allergens to nonlesional skin of AD patients, is a well-established model of an allergen-induced DTH-like skin immune response (20). In the case of a positive patch test, as observed in a subset of AD patients, CD4…”

Section: Discussionmentioning

confidence: 99%

“…Importantly, the development of a positive test reaction has been shown to strictly depend on the presence og IgE-bearing epidermal LC, circulating specific IgE Abs, and allergen-reactive T cells (20). The current explanation is that LC capture allergen via specific IgE molecules bound to the high affinity IgE receptor and subsequently induce an allergen-specific cutaneous T cell response (19).…”

Section: Discussionmentioning

confidence: 99%

“…To address this question, we investigated the expression of IL-16 in positive and negative skin reactions of clinically nonactive AD patients patchtested with the house dust mite Ag der p1, because this type of contact hypersensitivity is considered to involve IgE-dependent activation of epidermal LC (20). All patients were sensitized to der p1, as indicated by the presence of specific IgE Abs and previous skin testing.…”

Section: Il-16 Is Induced In Epidermal Dendritic Cells In Positive Atmentioning

confidence: 99%

“…Preferential presentation of these Ags subsequently leads to the induction of allergen-specific T cell responses that are capable of promoting IgE production and DTH responses (19). This understanding is supported by the clinical observation that DTH-like skin lesions can be provoked in a subset of AD patients by epicutaneous application of allergen (atopy patch test), with positive patch test reactions depending on the presence of IgE-bearing LC in the epidermis (20). However, the frequency of allergen-specific T cells in the atopy patch test model of acute AD is low (21), and the exact mechanisms regulating the influx of T cell into AD lesions remain to be defined.…”

mentioning

confidence: 92%

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Engagement of the FcεRI Stimulates the Production of IL-16 in Langerhans Cell-Like Dendritic Cells

Reich

Heine

Hugo

et al. 2001

The Journal of Immunology

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Preferential uptake and presentation of IgE-bound allergens by epidermal Langerhans cells (LC) via the high affinity IgE receptor, FcεRI, is regarded as an important mechanism in the induction of cutaneous inflammation in atopic dermatitis. Here, we show that activation of monocyte-derived LC-like dendritic cells (LLDC) through engagement of FcεRI induces the expression of IL-16, a chemoattractant factor for dendritic cells, CD4+ T cells, and eosinophils. We found that ligation of FcεRI on LLDC derived from atopic dermatitis patients that express high levels of FcεRI increases IL-16 mRNA expression and storage of intracellular IL-16 protein and enhances the secretion of mature IL-16 in a biphasic manner. An early release of IL-16 (peak at 4 h) is independent of protein synthesis, while a more delayed release (peak at 12 h) requires protein synthesis and occurs subsequent to the induction of IL-16 mRNA and intracellular accumulation of pro-IL-16. There was evidence that LLDC use caspase-1 to process IL-16, as inhibition of caspase-1, but not of caspase-3, partially prevented the release of IL-16 in response to ligation of FcεRI. In an in vivo model of IgE-dependent LC activation, the atopy patch test, positive skin reactions were also associated with the induction of IL-16 in epidermal dendritic cells. These data indicate that IL-16 released from LC after allergen-mediated activation through FcεRI may link IgE-driven and cellular inflammatory responses in diseases such as atopic dermatitis.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Il-16 Is Induced In Epidermal Dendritic Cells In Positive Atmentioning

confidence: 99%

mentioning

confidence: 92%

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Engagement of the FcεRI Stimulates the Production of IL-16 in Langerhans Cell-Like Dendritic Cells

Reich

Heine

Hugo

et al. 2001

The Journal of Immunology

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“…Por lo tanto, la captura de antígenos por par te de las células de Langerhans a través del FceRI constituye un posible puente entre la respuesta inflamatoria celular. Así mismo, la respuesta inflamatoria mediada por IgE y la activación de las células de Langerhans a través de NF-kB representa un mecanismo por el cual el FceRI expresado en monocitos y células dendríticas puede controlar las reacciones inflamatorias (96,97).…”

Section: Participación En La Respuesta Inflamatoria Epidérmicaunclassified

Las células de Langerhans en la inmunidad a leishmaniasis.

Zuluaga

Robledo

2004

biomedica

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En la respuesta inmune a parásitos del género Leishmania influyen múltiples factores entre los que se encuentran no sólo los relacionados con el parásito sino también aquéllos relacionados con el hospedero; de particular importancia es el tipo participante de célula presentadora de antígenos. En la piel, lugar donde el vector inocula el parásito, se encuentran las células de Langerhans que tienen como principal función servir como centinelas para la detección de microorganismos invasores. El estímulo que genera el microorganismo o las células circundantes induce la activación de las células de Langerhans, su maduración y migración hacia el tejido linfoide local (regional) donde presentan los antígenos a las células T para la posterior activación y diferenciación de subpoblaciones de células T específicas, responsables de la resolución de la infección o de la cicatrización. Se ha observado que durante la fase temprana de la infección con Leishmania hay muy pocas células T en el sitio de la infección lo cual sugiere que los macrófagos infectados tienen pocas probabilidades de encontrar allí células T con la especificidad requerida para eliminar el parásito y que, por tanto, son las células de Langerhans las que proporcionan la señal que activa las células T específicas para Leishmania en los ganglios linfáticos locales que drenan de la lesión e inducen su migración al sitio de la lesión. En la presente revisión se abordan las principales características de las células de Langerhans, se hace especial énfasis en la participación en la respuesta inflamatoria cutánea y se presentan los hallazgos más relevantes del papel de estas células en el modelo de infección por parásitos del género Leishmania.Palabras clave: células de Langerhans, células dendríticas, Leishmania, respuesta inmune.

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