Clinical and Laboratory Diagnostic Characteristics and Cytotoxigenic Potential of Hafnia alvei and Hafnia paralvei Strains

Abbott, Sharon L.; Moler, Silvia; Green, Nicole; Tran, Robert K.; Wainwright, Katlyn; Janda, J. Michael

doi:10.1128/jcm.00866-11

Cited by 42 publications

(25 citation statements)

References 21 publications

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“…Similarly, Hafnia spp. was reported to be resistant to CEF and sensitive to GEN, as found in this study with isolate 79M, however TET and CHL resistance have been previously reported [ 82 ] again implying that Hafnia spp. are also able to obtain further antibiotic resistant mechanisms.…”

Section: Discussionsupporting

confidence: 53%

Phylogenetic Analysis and Antimicrobial Profiles of Cultured Emerging Opportunistic Pathogens (Phyla Actinobacteria and Proteobacteria) Identified in Hot Springs

Jardine

Abia

Mavumengwana

et al. 2017

IJERPH

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Hot spring water may harbour emerging waterborne opportunistic pathogens that can cause infections in humans. We have investigated the diversity and antimicrobial resistance of culturable emerging and opportunistic bacterial pathogens, in water and sediment of hot springs located in Limpopo, South Africa. Aerobic bacteria were cultured and identified using 16S ribosomal DNA (rDNA) gene sequencing. The presence of Legionella spp. was investigated using real-time polymerase chain reaction. Isolates were tested for resistance to ten antibiotics representing six different classes: β-lactam (carbenicillin), aminoglycosides (gentamycin, kanamycin, streptomycin), tetracycline, amphenicols (chloramphenicol, ceftriaxone), sulphonamides (co-trimoxazole) and quinolones (nalidixic acid, norfloxacin). Gram-positive Kocuria sp. and Arthrobacter sp. and gram-negative Cupriavidus sp., Ralstonia sp., Cronobacter sp., Tepidimonas sp., Hafnia sp. and Sphingomonas sp. were isolated, all recognised as emerging food-borne pathogens. Legionella spp. was not detected throughout the study. Isolates of Kocuria, Arthrobacter and Hafnia and an unknown species of the class Gammaproteobacteria were resistant to two antibiotics in different combinations of carbenicillin, ceftriaxone, nalidixic acid and chloramphenicol. Cronobacter sp. was sensitive to all ten antibiotics. This study suggests that hot springs are potential reservoirs for emerging opportunistic pathogens, including multiple antibiotic resistant strains, and highlights the presence of unknown populations of emerging and potential waterborne opportunistic pathogens in the environment.

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Section: Discussionsupporting

confidence: 53%

Phylogenetic Analysis and Antimicrobial Profiles of Cultured Emerging Opportunistic Pathogens (Phyla Actinobacteria and Proteobacteria) Identified in Hot Springs

Jardine

Abia

Mavumengwana

et al. 2017

IJERPH

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“…Phylogenetic relationships among Enterobacteriaceae based on ampC sequence analysis, which is an accurate taxonomic assignment tool as observed previously (10,11,12), pointed out the evolutionary relatedness of CDA-1 to the chromosome-borne ␤-lactamases of Enterobacter aerogenes, Lelliottia nimipressuralis, and Citrobacter freundii (Fig. 2).…”

Section: Figmentioning

confidence: 99%

Characterization of a Novel AmpC β-Lactamase Produced by a Carbapenem-Resistant Cedecea davisae Clinical Isolate

Ammenouche

Dupont

Mammeri

2014

Antimicrob Agents Chemother

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A Cedecea davisae isolate, which was intermediate or resistant to third-generation cephalosporins and carbapenems, was recovered from a urine sample. Susceptibility testing, isoelectric focusing, and analysis of outer membrane proteins showed that AmpC ␤-lactamase expression combined with porin deficiency accounted for the carbapenem resistance. A cloning experiment followed by phenotypic and enzymatic characterization identified a novel class C enzyme that was phylogenetically and biochemically close to the chromosome-borne ␤-lactamases of the genera Enterobacter and Citrobacter. C edecea davisae is an enterobacterium that can be responsible for human infections (1-4). Cephalothin resistance has been already reported in this species, but the mechanism has not been yet elucidated (5).The aim of this work was to identify the mechanisms of resistance of a C. davisae clinical isolate that was resistant to almost all ␤-lactams. It was recovered from a 77-year-old woman who was hospitalized in an intensive care unit of the teaching hospital of Amiens. Eighteen days after a cardiac valve replacement, the patient had a hemorrhagic shock due to retroperitoneal bleeding. The urine sample recovered after nephrostomy was sent to the Laboratory of Bacteriology. It grew a Gram-negative rod (10 7 CFU/ml) that was identified as C. davisae FUR by matrix-assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) (Shimadzu, Marne la Vallée, France) and 16S rRNA sequencing. The patient was started successfully on intravenous ciprofloxacin for 7 days.

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“…H. alvei and M. morganii are considered to be intrinsically resistant to ampicillin, amoxicillin-clavulanic acid, and ampicillin-sulbactam, and the use of cefotaxime, ceftriaxone, or ceftazidime as monotherapy is discouraged against M. morganii due to the risk of selecting derepressed AmpC-resistant populations (30). In addition, M. morganii is considered intrinsically resistant to colistin and, as for H. alvei, a high percentage of colistin resistance has been described in wild-type isolates (30,31).…”

Section: (S)mentioning

confidence: 99%

Evaluation of the Accelerate Pheno System for Fast Identification and Antimicrobial Susceptibility Testing from Positive Blood Cultures in Bloodstream Infections Caused by Gram-Negative Pathogens

et al. 2017

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Bloodstream infections (BSI) are an important cause of morbidity and mortality. Increasing rates of antimicrobial-resistant pathogens limit treatment options, prompting an empirical use of broad-range antibiotics. Fast and reliable diagnostic tools are needed to provide adequate therapy in a timely manner and to enable a de-escalation of treatment. The Accelerate Pheno system (Accelerate Diagnostics, USA) is a fully automated test system that performs both identification and antimicrobial susceptibility testing (AST) directly from positive blood cultures within approximately 7 h. In total, 115 episodes of BSI with Gram-negative bacteria were included in our study and compared to conventional culture-based methods. The Accelerate Pheno system correctly identified 88.7% (102 of 115) of all BSI episodes and 97.1% (102 of 105) of isolates that are covered by the system's identification panel. The Accelerate Pheno system generated an AST result for 91.3% (95 of 104) samples in which the Accelerate Pheno system identified a Gram-negative pathogen. The overall category agreement between the Accelerate Pheno system and culture-based AST was 96.4%, the rates for minor discrepancies 1.4%, major discrepancies 2.3%, and very major discrepancies 1.0%. Of note, ceftriaxone, piperacillintazobactam, and carbapenem resistance was correctly detected in blood culture specimens with extended-spectrum beta-lactamase-producing Escherichia coli (n ϭ 7) and multidrug-resistant Pseudomonas aeruginosa (n ϭ 3) strains. The utilization of the Accelerate Pheno system reduced the time to result for identification by 27.49 h (P Ͻ 0.0001) and for AST by 40.39 h (P Ͻ 0.0001) compared to culture-based methods in our laboratory setting. In conclusion, the Accelerate Pheno system provided fast, reliable results while significantly improving turnaround time in blood culture diagnostics of Gramnegative BSI.KEYWORDS antimicrobial susceptibility testing, bloodstream infections, rapid tests B loodstream infections (BSI) are an important cause of morbidity and mortality, associated with prolonged hospital stays, as well as high costs for health care systems (1-3). Increasing rates of antimicrobial-resistant pathogens, in particular Gramnegative bacteria, limit treatment options, often prompting the empirical use of broad-range antibiotics. Therefore, we need fast and reliable diagnostic tools to speed up both identification (ID) and antimicrobial susceptibility testing (AST) from blood cultures in order to provide adequate therapy in a timely manner and to enable effective antibiotic stewardship interventions (4).

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Clinical and Laboratory Diagnostic Characteristics and Cytotoxigenic Potential of Hafnia alvei and Hafnia paralvei Strains

Cited by 42 publications

References 21 publications

Phylogenetic Analysis and Antimicrobial Profiles of Cultured Emerging Opportunistic Pathogens (Phyla Actinobacteria and Proteobacteria) Identified in Hot Springs

Phylogenetic Analysis and Antimicrobial Profiles of Cultured Emerging Opportunistic Pathogens (Phyla Actinobacteria and Proteobacteria) Identified in Hot Springs

Characterization of a Novel AmpC β-Lactamase Produced by a Carbapenem-Resistant Cedecea davisae Clinical Isolate

Evaluation of the Accelerate Pheno System for Fast Identification and Antimicrobial Susceptibility Testing from Positive Blood Cultures in Bloodstream Infections Caused by Gram-Negative Pathogens

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