Clinical and Microbiological Characteristics of Mycobacterium kansasii Pulmonary Infections in China

Guo, Yinjuan; Cao, Yanhua; Liu, Haican; Yang, Jinghui; Wei-ping, Wang; Wang, Bingjie; Li, Meilan; Yu, Fangyou

doi:10.1128/spectrum.01475-21

Cited by 13 publications

(22 citation statements)

References 40 publications

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“…Besides confirming the seven main species within the MKC in the largest dataset analyzed so far, our findings demonstrate that the M. kansasii species can be subdivided into four monophyletic lineages, which was similar as previously observed 13,18 . One of these, the major lineage (L1), is globally distributed and comprehending four successful clonal groups (sub-lineages L1.1.1, L1.1.2, L1.1.3 and L1.1.4).…”

Section: Discussionsupporting

confidence: 90%

“…The variability of virulence factors within MKC species is supported by earlier data, comparing M. kansasii against other species 6,17,18 suggesting an MKC species-specific virulome. To address this, we defined 287 M. kansasii genes orthologs to M. tuberculosis H37Rv potential virulence factors and screened for their presence in the genomes collection.…”

Section: Discussionsupporting

confidence: 68%

“…Even though some questions about the MKC pathogenicity, virulence, drug resistance, genetic diversity, and epidemiology have been addressed by analyzing genomic data 1,2,[6][7][8][9][10][12][13][14] , several aspects about the MKC species genomic characteristics and epidemiology remains unexplored. For instance, i) in addition to plasmids, the prevalence and characteristics of mobile genetic elements in MKC genomes, such as prophages; ii) the possible plasmids or prophages contribution to virulence or drug resistance; iii) the MKC pangenome structure and diversity; iv) the MKC viral defense mechanisms repertoire; v) the potential M. kansasii lineages and their association with certain geographical areas or niches; and vi) transmission mechanisms of the different MKC species.…”

Section: Introductionmentioning

confidence: 99%

“…Concerning the resistance of members of the MKC to antimicrobial agents, estimates are variable, but some recent studies conducted in China 10 and Brazil 11 estimated a high percentage (∼70%) of M. kansasii isolates resistant to ethambutol and ciprofloxacin, while between 10% and 20% of the isolates were resistant to rifampicin. However, in M. kansasii , no clear relation has been observed between mutations in particular genes and drug resistance as observed in M. tuberculosis 12 .…”

Section: Introductionmentioning

confidence: 99%

“…Due to the higher interest in isolating MKC from clinical rather that from environmental sources, and the strong association of M. kansasii with pulmonary disease, much more genomic sequencing data are available from the latter species, with the majority of data coming from Europe, China, and Australia leaving knowledge of other geographical areas lacking 8,10 . From M. kansasii genomic data, it was estimated that some lineages might be globally spread 8 , while genomes obtained from clinical isolates in Brazil and China 10,13 suggested at least four genetically diverse groups.…”

Section: Introductionmentioning

confidence: 99%

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Phylogenomic and genomic analysis reveals unique and shared genetic signatures of Mycobacterium kansasii complex species

Machado,

Vasconcellos,

Gomes

et al. 2024

Preprint

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Species belonging to the Mycobacterium kansasii complex (MKC) are frequently isolated from humans and the environment and can cause serious diseases. The most common MKC infections are caused by the species M. kansasii (stricto sensu), leading to tuberculosis-like disease. However, a broad spectrum of virulence, antimicrobial resistance and pathogenicity of these non-tuberculous mycobacteria (NTM) are observed across the MKC. Many genomic aspects of the MKC that relate to these broad phenotypes are not well elucidated. Here, we performed genomic analyses from a collection of 665 MKC strains, isolated from environmental, animal and human sources. We inferred the MKC pangenome, mobilome, resistome, virulome and defense systems and show that the MKC species harbors unique and shared genomic signatures. High frequency of presence of prophages and different types of defense systems was observed. We found that the M. kansasii species splits into four lineages, of which three are lowly represented and mainly in Brazil, while one lineage is dominant and globally spread. Moreover, we show that four sub-lineages of this most distributed M. kansasii lineage emerged during the 20th century. Further analysis of the M. kansasii genomes revealed almost 300 regions of difference contributing to genomic diversity, as well as fixed mutations that may explain the M. kansasii's increased virulence and drug resistance.

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Section: Discussionsupporting

confidence: 90%