Clinical and microbiological characterization of Clostridium difficile infection in Romania (2013-2014); a hospital-based study

Popescu, Gabriel Adrian; Şerban, Roxana; Pistol, Adriana; Niculcea, Andreea; Preda, Andreea; Lemeni, Daniela; Macovei, Ioana; Popoiu, Monica; Tenea, Cristina; Tălăpan, Daniela; Florea, Dragoș; Rafilă, Alexandru

doi:10.1186/1471-2334-14-s7-o24

Cited by 3 publications

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“…Although unexpectedly high, the increased prevalence of the 027 strains in the present study is concordant with the prevalence described in a larger study performed in 11 Romanian hospitals [18] and therefore is not likely to be considered, in our opinion, the result of sampling bias.…”

Section: Discussionsupporting

confidence: 88%

“…The detection of ribotype 027 described in the present study is very high compared to data from other countries and is probably related to the state of epidemic CDI in this region. Other studies revealed that 027 remained during the following years the dominant ribotype in Bucharest and in many other Romanian regions [18]. In one isolate (RT 433) only toxin B gene was detected, the gene for toxin A being undetectable.…”

Section: Discussionmentioning

confidence: 92%

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PCR coupled with mass-spectrometry for detection of Clostridium difficile virulence markers during the emergence of ribotype 027 in Bucharest area

Florea

Huhulescu

Indra

et al. 2015

Revista Romana De Medicina De Laborator

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Section: Discussionsupporting

confidence: 88%

Section: Discussionmentioning

confidence: 92%

PCR coupled with mass-spectrometry for detection of Clostridium difficile virulence markers during the emergence of ribotype 027 in Bucharest area

Florea

Huhulescu

Indra

et al. 2015

Revista Romana De Medicina De Laborator

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“…In the context of insufficient antibiotic susceptibility data available at this moment to describe Romanian C. difficile strains causing CDI, the present study was focused on characterizing the susceptibility patterns of toxigenic isolates belonging to some of the PRs identified in Romanian CDI outbreaks or in sporadical cases: 012, 014, 017, 018, 027, 046, 087 and 115 [25,28].…”

Section: Discussionmentioning

confidence: 99%

“…PRs of the isolates were identified in previous studies [25,28]. Seventeen strains belonging to PR 027 were included.…”

Section: In Vitro Antibiotic Susceptibility Testingmentioning

confidence: 99%

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Antibiotic susceptibility and resistance profiles of Romanian Clostridioides difficile isolates

Macovei¹,

Lemeni²,

Şerban³

et al. 2018

Revista Romana De Medicina De Laborator

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An Intra-Hospital Spread of Colistin-Resistant K. pneumoniae Isolates—Epidemiological, Clinical, and Genetic Analysis

Popivanov,

Markovska,

Gergova

et al. 2024

Medicina

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Background and Objective: Klebsiella pneumoniae appears to be a significant problem due to its ability to accumulate antibiotic-resistance genes. After 2013, alarming colistin resistance rates among carbapenem-resistant K. pneumoniae have been reported in the Balkans. The study aims to perform an epidemiological, clinical, and genetic analysis of a local outbreak of COLr CR-Kp. Material and Methods: All carbapenem-resistant and colistin-resistant K. pneumoniae isolates observed among patients in the ICU unit of Military Medical Academy, Sofia, from 1 January to 31 October 2023, were included. The results were analyzed according to the EUCAST criteria. All isolates were screened for blaVIM, blaIMP, blaKPC, blaNDM, and blaOXA-48. Genetic similarity was determined using the Dice coefficient as a similarity measure and the unweighted pair group method with arithmetic mean (UPGMA). mgrB genes and plasmid-mediated colistin resistance determinants (mcr-1, mcr-2, mcr-3, mcr-4, and mcr-5) were investigated. Results: There was a total of 379 multidrug-resistant K. pneumoniae isolates, 88% of which were carbapenem-resistant. Of these, there were nine (2.7%) colistin-resistant isolates in six patients. A time and space cluster for five patients was found. Epidemiology typing showed that two isolates belonged to clone A (pts. 1, 5) and the rest to clone B (pts. 2–4) with 69% similarity. Clone A isolates were coproducers of blaNDM-like and blaOXA-48-like and had mgrB-mediated colistin resistance (40%). Clone B isolates had only blaOXA-48-like and intact mgrB genes. All isolates were negative for mcr-1, -2, -3, -4, and -5 genes. Conclusions: The study describes a within-hospital spread of two clones of COLr CR-Kp with a 60% mortality rate. Clone A isolates were coproducers of NDM-like and OXA-48-like enzymes and had mgrB-mediated colistin resistance. Clone B isolates had only OXA-48-like enzymes and intact mgrB genes. No plasmid-mediated resistance was found. The extremely high mortality rate and limited treatment options warrant strict measures to prevent outbreaks.

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Clinical and microbiological characterization of Clostridium difficile infection in Romania (2013-2014); a hospital-based study

Cited by 3 publications

References 0 publications

PCR coupled with mass-spectrometry for detection of Clostridium difficile virulence markers during the emergence of ribotype 027 in Bucharest area

PCR coupled with mass-spectrometry for detection of Clostridium difficile virulence markers during the emergence of ribotype 027 in Bucharest area

Antibiotic susceptibility and resistance profiles of Romanian Clostridioides difficile isolates

An Intra-Hospital Spread of Colistin-Resistant K. pneumoniae Isolates—Epidemiological, Clinical, and Genetic Analysis

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