Clinical and Phylogenetic Characteristics of <i>Escherichia coli</i> Urinary Tract Infections

Lee, Ji Eun; Lee, Youn Hee; Nam, Chan Hee; Kwak, Ga Young; Lee, Soo Young; Kim, Jong Hyun; Hur, Jae Kyun; Kang, Jin Han

doi:10.14776/kjpid.2010.17.1.16

Cited by 3 publications

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“…Phylogenetic groups and VFs are unique microbiological features of the E. coli strains, but these features are obviously influenced by the host genetic factors (immune status, specific blood group antigens, and dietary intake), and socioenvironmental factors (climate, economic condition, and the extent of antibiotics usage)11,19,20).…”

Section: Discussionmentioning

confidence: 99%

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Regional differences in phylogenetic group ofEscherichia colistrains isolated from children with urinary tract infection in Korea

et al. 2012

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PurposeWe phylogenetically analyzed the Escherichia coli strains isolated from children with urinary tract infection (UTI) in 2 regions of Korea. Virulence factors (VFs) and antibiotic resistance of the strains were also determined to compare the possible differences.MethodsA total of 138 E. coli strains were collected from the 2 regions; Gyeongin (78 strains) and Gyeongnam (60 strains). The phylogenetic groups were determined using the triplex polymerase chain reaction (PCR) method and multiplex PCRs were used to detect 7 VFs genes (fimH, papC, iutA, hlyA, sfa/focDE, afa/draBC, and kpsMT II). We also tested for antibiotic resistance.ResultsPhylogenetic groups, B2 (61.6%) and D (26.8%), comprised the majority of all isolated strains. Regional comparisons revealed that more B2 strains and fewer non-B2 (A+B1+D) strains were found in Gyeongnam, than in the Gyeongin region (P=0.033), and certain VFs were predominantly detected in Gyeongnam (P<0.05). Neither regional nor phylogenetic differences, in antibiotic resistance of the strains, were significant.ConclusionWe were able to confirm that the geographic location is an important determinant of the distribution of the phylogenetic groups and VFs among the E. coli strains that cause UTI in children.

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Section: Discussionmentioning

confidence: 99%

“…Phylogenetic research on E. coli strains has been preformed not only in the field of medical science, but also in veterinary medicine and public health science7-9). However, there are a few phylogenetic studies on ExPEC strains conducted in children, especially in Korea10,11).…”

Section: Introductionmentioning

confidence: 99%

Regional differences in phylogenetic group ofEscherichia colistrains isolated from children with urinary tract infection in Korea

et al. 2012

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Molecular typing of uropathogenicEscherichia coliisolated from Korean children with urinary tract infection

Yun

Kim

et al. 2015

Korean J Pediatr

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PurposeWe investigated the molecular types of uropathogenic Escherichia coli (UPEC) by using conventional phylogrouping, multilocus sequence typing (MLST), and fimH genotyping.MethodsSamples of patients younger than 18 years of age were collected from the Chung-Ang University Hospital over 2 years. Conventional phylogenetic grouping for UPEC strains was performed by polymerase chain reaction (PCR). Bacterial strain sequence types (STs) were classified on the basis of the results of partial sequencing of seven housekeeping genes. In addition, we analyzed nucleotide variations in a 424-base pair fragment of fimH, a major virulence factor in UPEC.ResultsSixty-four UPEC isolates were analyzed in this study. Phylogenetic grouping revealed that group B2 was the most common type (n=54, 84%). We identified 16 distinctive STs using MLST. The most common STs were ST95 (35.9%), ST73 (15.6%), ST131 (12.5%), ST69 (7.8%), and ST14 (6.3%). Fourteen fimH allele types were identified, of which 11 had been previously reported, and the remaining three were identified in this study. f1 (n=28, 45.2%) was found to be the most common allele type, followed by f6 and f9 (n=7, 11.3% each). Comparative analysis of the results from the three different molecular typing techniques revealed that both MLST and fimH typing generated more discriminatory UPEC types than did PCR-based phylogrouping.ConclusionWe characterized UPEC molecular types isolated from Korean children by MLST and fimH genotyping. fimH genotyping might serve as a useful molecular test for large epidemiologic studies of UPEC isolates.

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UropathogenicEscherichia coliST131 in urinary tract infections in children

et al. 2017

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PurposeEscherichia coli sequence type (ST) 131, a multidrug-resistant clone causing extraintestinal infections, has rapidly become prevalent worldwide. However, the epidemiological and clinical features of pediatric infections are poorly understood. We aimed to explore the characteristics of ST131 Escherichia coli isolated from Korean children with urinary tract infections.MethodsWe examined 114 uropathogenic E. coli (UPEC) isolates from children hospitalized at Chung-Ang University Hospital between 2011 and 2014. Bacterial strains were classified into STs by partial sequencing of seven housekeeping genes (adk, fumC, gyrB, icd, mdh, purA, and recA). Clinical characteristics and antimicrobial susceptibility were compared between ST131 and non-ST131 UPEC isolates.ResultsSixteen UPEC isolates (14.0%) were extended-spectrum β-lactamase (ESBL)-producers; 50.0% of ESBL-producers were ST131 isolates. Of all the isolates tested, 13.2% (15 of 114) were classified as ST131. There were no statistically significant associations between ST131 and age, sex, or clinical characteristics, including fever, white blood cell counts in urine and serum, C-reactive protein, radiologic abnormalities, and clinical outcome. However, ST131 isolates showed significantly lower rates of susceptibility to cefazolin (26.7%), cefotaxime (40.0%), cefepime (40.0%), and ciprofloxacin (53.3%) than non-ST131 isolates (65.7%, 91.9%, 92.9%, and 87.9%, respectively; P<0.001 for all). ESBL was more frequently produced in ST131 (53.3%) than in non-ST131 (8.1%) isolates (P<0.01).ConclusionST131 E. coli isolates were prevalent uropathogens in children at a single medical center in Korea between 2011 and 2014. Although ST131 isolates showed higher rates of antimicrobial resistance, clinical presentation and outcomes of patients were similar to those of patients infected with non-ST131 isolates.

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Clinical and Phylogenetic Characteristics of Escherichia coli Urinary Tract Infections

Cited by 3 publications

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Regional differences in phylogenetic group ofEscherichia colistrains isolated from children with urinary tract infection in Korea

Regional differences in phylogenetic group ofEscherichia colistrains isolated from children with urinary tract infection in Korea

Molecular typing of uropathogenicEscherichia coliisolated from Korean children with urinary tract infection

UropathogenicEscherichia coliST131 in urinary tract infections in children

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