Molecular typing of uropathogenicEscherichia coliisolated from Korean children with urinary tract infection

Abstract

PurposeWe investigated the molecular types of uropathogenic Escherichia coli (UPEC) by using conventional phylogrouping, multilocus sequence typing (MLST), and fimH genotyping.MethodsSamples of patients younger than 18 years of age were collected from the Chung-Ang University Hospital over 2 years. Conventional phylogenetic grouping for UPEC strains was performed by polymerase chain reaction (PCR). Bacterial strain sequence types (STs) were classified on the basis of the results of partial sequencing of seven housekeeping genes. In addition, we analyzed nucleotide variations in a 424-base pair fragment of fimH, a major virulence factor in UPEC.ResultsSixty-four UPEC isolates were analyzed in this study. Phylogenetic grouping revealed that group B2 was the most common type (n=54, 84%). We identified 16 distinctive STs using MLST. The most common STs were ST95 (35.9%), ST73 (15.6%), ST131 (12.5%), ST69 (7.8%), and ST14 (6.3%). Fourteen fimH allele types were identified, of which 11 had been previously reported, and the remaining three were identified in this study. f1 (n=28, 45.2%) was found to be the most common allele type, followed by f6 and f9 (n=7, 11.3% each). Comparative analysis of the results from the three different molecular typing techniques revealed that both MLST and fimH typing generated more discriminatory UPEC types than did PCR-based phylogrouping.ConclusionWe characterized UPEC molecular types isolated from Korean children by MLST and fimH genotyping. fimH genotyping might serve as a useful molecular test for large epidemiologic studies of UPEC isolates.

Drug resistance and plasmid profile of uropathogenic Escherichia coli among urinary tract infection patients in Addis Abeba

“…The participants in our study had at least two of the following urologic symptoms; the most frequent complaint was dysuria followed by urine urgency, urine incontinence, flank pain, suprapubic pain, and a few with fever and chills. These findings are in agreement with results from studies conducted in South Korea [21] and Nigeria [24].…”

“…No drug resistance was found to Meropenem and Imipenem, and this result has also been reported from South Korea [21], China [16]and Iran [28]. Low resistance was observed for Ciprofloxacin (14.5%), Nitrofurantoin (5%), Norfloxacin (15%) and Amikacin (2.5%) which is comparable to other studies in Ethiopia [7,29] and Iran [11,28].…”

“…The overall incidence of antibiotic resistance in the E. coli in this study was high and almost all of the strains were resistant to two or more antibiotics. This result is comparable to the high rates of resistance found in India (92.5%) [19], (100%) [20], China (78.1%) [16], (82.6%) [25], but quite a lot higher than observed in South Korea (21.9%) [21], Mexico (30.2%) [22] and another study in India (50%) [23]. The high prevalence of multidrug resistance E. coli strains in our study may be explained by the fact that he majority of the isolates has been exposed to several.…”

Multi Locus VNTR (MLVA) Typing and Detection of the OI-122 Pathogenicity Island in Typical and Atypical Enteropathogenic Escherichia coli Isolated from Children with Acute Diarrhea

“…The significant negative association with diarrhea for the yjaA gene reported in the study of Afset et al (6) and suggested that the presence of the yjaA gene may be a marker for low diarrheagenic potential aEPEC strains. The yjaA gene as the negative marker for diarrhea may be one possible reason for the association of B2 phylogenetic group with extraintestinal E. coli infections (20)(21)(22).…”

Virulence Factors and Phylogenetic Characteristics of Uropathogenic <i>Eschericihia coli</i> Strains Isolated in Saratov

“…последовательности праймеров, использованных в данной работе для амплификации фрагментов генов fimH, chuA, yjaA и участка днк TspE4.C2, описаны ранее в работе K.W. Yan et al [8], а последовательности праймеров для амплификации фрагментов генов pap, sfa, afa, iha, irp2, iuc, iroN, hlyA, vat, usp, set-1, kpsMT, iss, cva, ompT -в работе H. Momtaz et al [9]. при проведении пцр использовали 10-кратный пцр буфер б (зао «синтол», россия), 25 ммоль раствор днтФ (зао «синтол», россия), 25 ммоль раствор MgCl 2 (зао «синтол», россия), SynTaq днк-полимеразу (зао «синтол», россия).…”