бешенство является зоонозной природно-очаговой инфекционной болезнью, общей для челове-ка и животных. ее абсолютная фатальность и эволю-ция придают чрезвычайный характер каждому случаю и ставят ветеринарно-медицинскую проблему бешен-ства в разряд первостепенных. ежегодная инцидент-ность регистрируемого в мире бешенства животных составляет от 30 до 45 тыс. [6]. количество людей, по-гибающих от гидрофобии, достигает 55 тыс. человек, из них 45 % случаев относится к региону Южной и Юго-восточной азии [20,21]. в российской Федерации на протяжении по- в статье представлены результаты изучения биопроб специалистами центра специальной лабораторной диа-гностики особо опасных и экзотических инфекционных болезней за период с 2002 по 2015 год, поступивших от погибших людей, подозрительных на инфицирование вирусом бешенства. освещены основные трудности при работе с поступившими пробами биологического материала от погибших людей и пути их преодоления. обозначены острые проблемы по бешенству эпизоотологической и эпидемиологической направленности, требую-щие комплексного решения со стороны органов здравоохранения и ветеринарной службы российской Федерации. подведены итоги лабораторно-диагностической работы центра специальной лабораторной диагностики особо опасных и экзотических инфекционных заболеваний. показано, что применяемые методы выделения и иденти-фикации возбудителя бешенства позволяют качественно и своевременно диагностировать данное заболевание.Ключевые слова: бешенство, уличный вирус бешенства, лабораторная диагностика. Scientific Center on Expertise of Medical Application Products, Moscow, Russian FederationThe article presents the results of bioassays investigation by the specialists of the Center for Special Laboratory Diagnostics of Particularly Dangerous and Exotic Infectious Diseases, isolated during 2002-2015, obtained from deceased people suspected for rabies virus infection. Outlined are the main challenges of working with the received samples of biological material from dead people and ways of handling these issues. Put forward are the pressing problems of epizootic and epidemiological bias that require comprehensive solutions on the part of healthcare authorities and the veterinary service of the Russian Federation. Given are the results of the laboratory-diagnostic activities of the Center for Special Laboratory Diagnostics of Particularly Dangerous and Exotic Infectious Diseases, which indicate that the applied methods of isolation and identification of the rabies pathogen allow for efficient and in-time diagnosis of the disease.

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The review considers the relevant aspects of laboratory diagnosis of rabies. The methods of laboratory diagnostics of rabies infection, standardized by WHO in 2018, and their use in the Russian Federation are presented. The scheme of laboratory diagnostics of rabies, applied by specialists of the “48th CRI” of the Ministry of Defense of Russia, for the study of biological samples from deceased people is outlined. Between 2002 and 2018, the study of biomaterial was carried out using molecular-biological, virological methods of diagnosis and in some cases electron microscopy, which allowed to detect and identify the pathogen in 257 samples from 71 people, to certify and deposit new isolates of the rabies virus. Accumulation and analysis of the lessons learned in the application of molecular-biological method of rabies diagnosis allows us to recommend the use of RT-PCR, real-time RT-PCR sets (having a certificate of state registration) in healthcare and veterinary medicine practice to identify the causative agent of rabies infection. The use of molecular-biological methods is promising in terms of the development of rabies diagnosis to improve epidemiological surveillance and raise the efficiency of the biological protection of the population of the Russian Federation.

We present the midrapidity charged pion invariant cross sections and the ratio of π − -to-π + production (5 < pT < 13 GeV/c), together with the double-helicity asymmetries (5 < pT < 12 GeV/c) in polarized p+p collisions at √ s = 200 GeV. The cross section measurements are consistent with perturbative calculations in quantum chromodynamics within large uncertainties in the calculation due to the choice of factorization, renormalization, and fragmentation scales. However, the theoretical calculation of the ratio of π − -to-π + production when considering these scale uncertainties overestimates the measured value, suggesting further investigation of the uncertainties on the chargeseparated pion fragmentation functions is needed. Due to cancellations of uncertainties in the charge ratio, direct inclusion of these ratio data in future parameterizations should improve constraints on the flavor dependence of quark fragmentation functions to pions. By measuring charge-separated pion asymmetries, one can gain sensitivity to the sign of ∆G through the opposite sign of the up and down quark helicity distributions in conjunction with preferential fragmentation of positive pions from up quarks and negative pions from down quarks. The double-helicity asymmetries presented are sensitive to the gluon helicity distribution over an x range of ∼0.03-0.16.

West Nile virus (WNV) circulation in the territory of Saratov region and its role in the infectious pathology were investigated. For this purpose, in studies conducted in 2013-2015, suspensions of bloodsucking arthropods, organs of birds and small mammals were analyzed for the presence of WNV markers (antigens and/or RNA). The seroprevalence level in live-stock animals and population of the region was evaluated; clinical samples from patients with symptoms compatible with West Nile fever (WNF) were analyzed. As a result of the investigations, WNV markers were detected in field samples gathered in natural biotopes and in the city of Saratov. Immunity to WNV was detected in horses. A stable domain of persons with immunity to this agent was revealed among regional population. Patients with WNF have been annually registered in the region since 2012. The obtained results confirm active circulation of WNF in the Saratov region, as well as formation of stable natural and anthropourgic foci.