Clinical application and analysis of hepatitis C virus NS3 antigen detection by ELISA in human serum

The scavenger receptors SRA-1 and SREC-I cooperate with TLR2 in the recognition of the hepatitis C virus non-structural protein 3 by dendritic cells

“…Our study demonstrates the involvement of SRs in the cross-presentation of the viral antigen NS3. As soluble NS3 is detectable in the serum of chronically infected patients [38], its ability to be spontaneously cross-presented by DCs via SRs may favour the generation of NS3-specific effector T cells.…”

Hepatitis C virus window‐phase infections: closing the window on hepatitis C virus

“…There are problems associated with the technical complexity, capital equipment, and consumable costs, particularly with respect to their introduction in low‐development index countries. An attractive alternative strategy for many countries would be to use assays that detect viral antigens, provided they are of sufficient sensitivity, as has recently been proposed for China 12,13 …”

“…An attractive alternative strategy for many countries would be to use assays that detect viral antigens, provided they are of sufficient sensitivity, as has recently been proposed for China. 12,13 The precise relationship between viral load as determined by antigenemia and by NAT is unknown but it has been suggested that there is an approximately linear correlation. 11,14,15 Previous studies have reported the use of an HCV antigen-only assay (Trak-C, Ortho-Clinical Diagnostics, Inc.), which had a sensitivity for detection equivalent to a RNA viral load of 100,000 IU per mL.…”

Characterization and Application of Monoclonal Antibody Against Hepatitis C Virus Nonstructual Protein Three

“…(17)(18)(19) Therefore, it is very possible to find two monoclonal antibodies against NS 3 protein with different epitopes, which is very important to establish the HCV detection assay. The assay that we want to develop is an ELISA for antigen detection sandwiched between two monoclonal antibodies.…”