Clinical Application of the DiversiLab Microbial Typing System Using Repetitive Sequence‐Based PCR for Characterization of Helicobacter pylori in Japan

Abstract

We evaluated the DiversiLab (DL) system with universal primers, a semiautomated repetitive extragenic palindromic sequence-based polymerase chain reaction (PCR) (rep-PCR) system, for the characterization of Helicobacter pylori in Japan. All 135 isolates from Japanese patients with gastric cancer (GC, n = 55) or non-GC (n = 80) were used and subjected to the drug susceptibility examinations (amoxicillin, AMPC; metronidazole, MNZ; and clarithromycin, CAM) by E-test. There were 28 MNZ-resistant (20.7%), 35 CAM-resistant (25.9%), and 16 MNZ/CAM-resistant (11.9%) isolates. DL rep-PCR fingerprinting analysis at the level of 95% similarity revealed five major groups (A-E) and the other including 45 isolates. The occupation rates of GC-derived isolates in groups B (54.2%) and E (58.8%) were higher than in the other groups: A (26.7%), C (28.6%), D (30.0%), and the other (40.0%). Relative higher occupation rates of drug resistants, such as MNZ-, CAM- and double MNZ/CAM-resistant isolates, were observed in groups B (45.8%), C (42.6%), and D (40%). Five of eight GC-derived isolates with MNZ/CAM resistance were significantly assigned to group B (P = 0.0312, χ(2) -test). These results suggest that the isolates classified in group B have a potential to contribute to the development of severe gastric disorders. The DL system, rapid and high sensitive technology, would be widely available in clinical laboratory for pathological and epidemiological analyses even in H. pylori.

Plasma Levels of Homocysteine and the Occurrence and Progression of Rectal Cancer

“…Worldwide, colorectal cancer is the second most common form of cancer diagnosed in women and the third most common cancer in men [ 1 ]. Although the incidence and mortality rates of this type of cancer are now declining due to the development of novel diagnostic methods and treatments, early-stage rectal cancer is still difficult to diagnose [ 2 – 5 ]. Colonoscopy, followed by biopsy and histopathology, are considered to be the gold standard for the diagnosis of rectal cancer.…”

Minimum inhibitory concentrations of commonly used antibiotics against Helicobacter Pylori: A multicenter study in South China

“…While the resistance rate of H . pylori to MTZ is relatively low in Southern Europe, Taiwan, and Japan (less than 20%) [ 34 – 36 ], it is high in Africa (75.8%) [ 13 ] and China (78.2%) [ 37 ]. The main reasons for the high MTZ resistance are its previous prescription for parasitic or gynecological infections [ 38 ], extensive availability, and affortability.…”