Clinical Applications of a Gas Chromatographic Method for the Combined Determination of Testosterone and Epitestosterone Glucuronide in Urine

Futterweit, Walter; Freeman, Ruth; Siegel, George L.; Griboff, Solomon I.; Dorfman, Ralph I.; Soffer, Louis J.

doi:10.1210/jcem-25-11-1451

Cited by 21 publications

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“…Since testosterone is the most potent natural androgenic hormone and the adrenal is capable of synthesizing testosterone (4), increased secretion of this steroid has been suggested as the responsible mechanism. This hypothesis is supported by the demonstration of increased urinary excretion (5)(6)(7)(8)(9)(10)(11), plasma concentration (10,(12)(13)(14), and production rate (10)(11)(12)(13)(14)(15)(16)(17)(18)(19) of testosterone in patients with congenital adrenal hyperplasia. However, recent work by Korenman and Lipsett (15) and Horton and Tait (16) indicates that the weak androgen, androstenedione (androst-4-ene-3, 17- ther demonstrated that almost two-thirds of blood testosterone in the normal female is derived from blood androstenedione.…”

Section: Introductionmentioning

confidence: 87%

Androstenedione and Its Conversion to Plasma Testosterone in Congenital Adrenal Hyperplasia*

Horton¹,

Frasier²

1967

J. Clin. Invest.

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Summary. The plasma concentration, production rate, and conversion ratio of androstenedione and testosterone were studied in seven children with congenital adrenal hyperplasia (CAH) of the 21-hydroxylase type. Plasma androstenedione and testosterone measured by double isotope derivative assay and estimated blood production rates were manyfold increased in the untreated state, markedly suppressed with glucocorticoid, and increased after the administration of ACTH.The metabolic clearance rate when corrected for body size and the conversion ratio of androstenedione to testosterone were similar to previously determined values in normal adults. Consideration of the androgen concentrations and conversion ratios indicates that in children with CAH, 76% of the plasma testosterone in prepubertal females and 36% in males are derived from peripheral conversion of blood androstenedione. The calculated amount of testosterone unaccounted for by peripheral conversion is similar to normal prepubertal values. This approach indicates that virilization in these children results from increased levels of testosterone but that the major source in CAH of this potent androgen is androstenedione secreted by the adrenal cortex.

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