Histometric assessment of human testicular biopsies

Dykes, J. R. W.

doi:10.1002/path.1710970303

Cited by 29 publications

(24 citation statements)

References 10 publications

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“…The aim of our study was to investigate testicular biopsies of oligo-or azoospermie males by means of the Johnsen method and the time-consuming point-count method [4,5,7], and to compare these meth ods with each other and simultaneously with the results of endocrinological investigations of these cases.…”

mentioning

confidence: 99%

Correlation of Hormone Levels and Quantitative Histological Findings in Testicular Biopsies

Mikuz¹,

Leitner²,

Scheiber³

et al. 1985

Eur Urol

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mentioning

confidence: 99%

Correlation of Hormone Levels and Quantitative Histological Findings in Testicular Biopsies

Mikuz¹,

Leitner²,

Scheiber³

et al. 1985

Eur Urol

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“…It is indeed well known that patients with advanced liver disease show testicular atrophy and morphological and morphometric changes in alcoholic and nonalcoholic liver disease in humans have been described by several authors [17][18][19][20] . A rat model for alchohol-induced gonadal atrophy has also been reported 21 .…”

Section: Discussionmentioning

confidence: 99%

“…At doses ≥ 1 g/ kg bw/day, DBP is a testicular toxicant in rodents, causing seminiferous tubule degeneration, with young animals being more susceptible than adults to this toxic effect 10 . At lower dose levels (≥ 250 mg/kg/day), when administered during the major period of male reproductive organogenesis in the rat (gestation days [12][13][14][15][16][17][18][19][20][21], DBP blocks male reproductive development, resulting in feminization of male pups, malformation of the male reproductive organs, seminiferous tubule degeneration, and Leydig cell hyperplasia and adenomas 11 . DBP is hydrolyzed by lipase to mono-n-butyl phthalate, which is thought to be the active form 12 .…”

Section: Introductionmentioning

confidence: 99%

Di-n-butyl Phthalate is Toxic to the Male Reproductive System and Its Toxicity is Enhanced by Thioacetamide Induced Liver Injury

et al. 2004

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Abstract:The modifying effects of liver injury on male reproductive organ toxicity of di-n-butyl phthalate (DBP) were studied using male F344 rats. Seventy-two male animals, aged 10 weeks at the commencement, were divided into 8 groups of 9 rats each. Groups 1 to 4 were given 200 mg/kg bw of thioacetamide (TAA) intraperitoneally 3 times / week, while Groups 5 to 8 were injected with the PBS vehicle only. From 1 week after the start, groups 1 and 5, 2 and 6, and 3 and 7 were given daily 500, 125, 31.25 mg/kg bw of DBP, respectively (23 times) for 4 weeks, while groups 4 and 8 served as vehicle controls. As the result of our treatment, liver injury was induced in the animals, and the liver injury was confirmed by histopathological findings and serum transaminase levels at sacrifice. Enhancement of rat male reproductive organ toxicity of DBP under liver injury status was also seen. The relative organ weights of prostate, bilateral seminal vesicles, bilateral epididymides and bilateral testes, and the sperm number and motility rate were decreased. Also the sperm abnormality rate was increased under liver injury. Furthermore histopathological abnormality of the testis, such as absence of germ cells and the presence of Sertoli cells only was seen in the rats treated with TAA and high dose of DBP, however neither the serum nor testicular testosterone levels varied in the present study. The sperm number and relative weight of bilateral epididymides were decreased by TAA treatment only, unrelated to DBP treatment. These facts suggest that liver injury can not only enhance the rat male reproductive organ toxicity of DBP, but also induce such toxicity by itself. (J Toxicol Pathol 2004; 17: 177-185)

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“…The testicular cells (spermatogonia type A & B, primary spermatocytes cells, LC and SC) of each rat testis were counted under Olympus BX-40 microscope supported with an Image Pro Plus program. The mean counts of each cell type were recorded per section for each group [12,21,22].…”

Section: Cells Quantitationmentioning

confidence: 99%

Testicular Changes in Male Albino Rat Pups Exposed to Medroxy- Progesterone Acetate during Lactational Period

Ahmed¹,

To²,

As³

et al. 2017

J Steroids Horm Sci

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Citation: Ahmed SI, Ali TO, Elsheikh AS, Attia GA, Abdalla AM, et al. (2017) AbstractThere are great concerns regarding the use of synthetic progesterone during breastfeeding due to probable negative effects on future fecundity of male infants. Therefore the present study was conducted to evaluate the effects of exposing male rat pups to depot medroxyprogesterone acetate (DMPA) during lactational period on pubertal testicular histology, morphometry and cells quantitation. Twenty male Wistar rat pups reared to dams treated with DMPA (10 mg/ kg BW) every other day during their early lactation period; were employed to achieve the objectives of this study. Other 20 male rat pups reared to untreated dams served as control. The pups were allowed to reach 90 days old, sacrificed, their testes were dissected and weighed and histological sections were prepared.The results showed that exposing male rat pups to DMPA during the lactational period significantly (P<0.001) affected their testicular histology, morphometry and the quantities of testicular cells. The thickness of the germinal epithelium (GE) and the diameter of the seminiferous tubules (ST) were reduced; while the interstitial space (IS) thicknesses were increased. The testicular cells of the rats reared on dams treated with DMPA experienced varying degrees of apoptosis and count reduction. The ST appeared with unusual configuration with detached and/or folded basal lamina, it has few germinal layers, decreased Sertoli cells (SC) and their lumen contained cells debris and very few sperms; while the wide IS contained few Leydig cells (LC). Exposure to DMPA during the lactational period adversely affects the testicular structure. Thus foretells a negative impact on future fertility. Materials and Methods MaterialsExperimental animals: Ten weeks old 8 pregnant female Wistar albino rats were grouped into two groups (4 rats each) and kept separate away from any stress in sterilized polypropylene cages (90 cm × 45 cm × 15 cm) lined with woody husk. They were kept at 28 ± 7°C temperature in light/dark cycle (12:12 h), fed on commercial pellet and offered water ad libitum. After delivery 4 dams with 20 male pups (Group I) were injected with a placebo to serve as controls; while 4 dams with 20 male pups (Group II) were subcutaneously injected with 10 mg/kg BW of depot medroxy-progesterone acetate (DMPA; Depo-Provera®) every other day (on the first fifteen days) of lactational period. The dam body weights were taken on each day of injection to adjust the dose. The 40 male rat pups were allowed to grow for 90 days where they reach maturity [2,4,[10][11][12].Study design: This one factorial experiment study was designed to investigate the effects of lactational exposure to DMPA on suckling male rat's pups' testis histological structure, morphometry, cells quantities at Citation: Ahmed SI, Ali TO, Elsheikh AS, Attia GA, Abdalla AM, et al. (2017) Testicular Changes in Male Albino Rat Pups Exposed to MedroxyProgesterone Acetate during Lactational Period. J Steroids Horm Sc...

show abstract

Histometric assessment of human testicular biopsies

Cited by 29 publications

References 10 publications

Correlation of Hormone Levels and Quantitative Histological Findings in Testicular Biopsies

Correlation of Hormone Levels and Quantitative Histological Findings in Testicular Biopsies

Di-n-butyl Phthalate is Toxic to the Male Reproductive System and Its Toxicity is Enhanced by Thioacetamide Induced Liver Injury

Testicular Changes in Male Albino Rat Pups Exposed to Medroxy- Progesterone Acetate during Lactational Period

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