Clinical applications of MS‐based protein quantification

Sabbagh, Bassel; Mindt, Sonani; Neumaier, Michael; Findeisen, Peter

doi:10.1002/prca.201500116

Cited by 32 publications

(20 citation statements)

References 250 publications

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“…Methods to achieve this separation include the use of antibody purification [27,28] or chromatographic separation (LC, UPLC) either in normal or reverse phase [29]. In the clinical laboratory, the method(s) used will depend upon the goal of the analysis, taking into consideration the costs and benefits associated with each methodology, reviewed in [21,24,25]. As technology advances, this pre-processing may become obsolete, reducing the time to analysis without compromising sensitivity and accuracy.…”

Section: Clinical Proteomic Technologymentioning

confidence: 99%

“…In proteomics, there are two primary methods by which quantitative data is achieved: unlabeled and labeled [21,24,25]. Labeled methods to quantitate proteins include incorporation of a small mass tag, examples of which include isotope coded affinity tags (ICAT) and isobaric tags for relative and absolute quantitation (iTRAQ).…”

Section: Clinical Proteomic Technologymentioning

confidence: 99%

“…For this reason, clinical proteomics will be most useful for diagnosis of diseases with rare or unknown etiology, monitoring the therapeutic effect and activity of drug regimens, improving treatment options for individual patients, and, as always, discovering a brave new world of medical possibilities. Comparisons between proteomic analysis and immunoassays have been discussed elsewhere [24,32,63–81]; hence, this review will focus on the uses of proteomics in the clinical setting.…”

Section: Personalized Proteomicsmentioning

confidence: 99%

See 2 more Smart Citations

Personalized Proteomics: The Future of Precision Medicine

2016

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Medical diagnostics and treatment has advanced from a one size fits all science to treatment of the patient as a unique individual. Currently, this is limited solely to genetic analysis. However, epigenetic, transcriptional, proteomic, posttranslational modifications, metabolic, and environmental factors influence a patient’s response to disease and treatment. As more analytical and diagnostic techniques are incorporated into medical practice, the personalized medicine initiative transitions to precision medicine giving a holistic view of the patient’s condition. The high accuracy and sensitivity of mass spectrometric analysis of proteomes is well suited for the incorporation of proteomics into precision medicine. This review begins with an overview of the advance to precision medicine and the current state of the art in technology and instrumentation for mass spectrometry analysis. Thereafter, it focuses on the benefits and potential uses for personalized proteomic analysis in the diagnostic and treatment of individual patients. In conclusion, it calls for a synthesis between basic science and clinical researchers with practicing clinicians to design proteomic studies to generate meaningful and applicable translational medicine. As clinical proteomics is just beginning to come out of its infancy, this overview is provided for the new initiate.

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Section: Clinical Proteomic Technologymentioning

confidence: 99%

Section: Clinical Proteomic Technologymentioning

confidence: 99%

Section: Personalized Proteomicsmentioning

confidence: 99%

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Personalized Proteomics: The Future of Precision Medicine

2016

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“…Nowadays, MS-based proteomic approaches have been used regularly in routine clinical diagnostic procedures, including the comprehensive characterization, classification, and identification of microorganisms [65–67]. Matrix-assisted laser desorption/ionization time-of-flight MS (MALDI-TOF MS) has been broadly adopted by many clinical microbiology laboratories over the past decade [68, 69].…”

Section: Ms-based Proteomics Progresses On Microbial Identification Amentioning

confidence: 99%

Proteomics progresses in microbial physiology and clinical antimicrobial therapy

Chen

Zhang

Wang

et al. 2016

Eur J Clin Microbiol Infect Dis

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Clinical microbial identification plays an important role in optimizing the management of infectious diseases and provides diagnostic and therapeutic support for clinical management. Microbial proteomic research is aimed at identifying proteins associated with microbial activity, which has facilitated the discovery of microbial physiology changes and host–pathogen interactions during bacterial infection and antimicrobial therapy. Here, we summarize proteomic-driven progresses of host–microbial pathogen interactions at multiple levels, mass spectrometry-based microbial proteome identification for clinical diagnosis, and antimicrobial therapy. Proteomic technique progresses pave new ways towards effective prevention and drug discovery for microbial-induced infectious diseases.

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“…The recent initiative of the Endocrine Society on the harmonization of results from large epidemiologic studies on male hypogonadism represents a milestone, as for the first time it established consensus reference intervals for testosterone [Travison et al, 2017]. Nonetheless, this goal is still far from being achieved for the other steroid and small-molecule hormones, and a much more challenging perspective is foreseeable for protein hormones [Sabbagh et al, 2016]. The techniques and assays used in clinical and research laboratories differ considerably.…”

mentioning

confidence: 99%

Normative Basal Values of Hormones and Proteins of Gonadal and Adrenal Functions from Birth to Adulthood

Fanelli¹,

Baronio

Ortolano

et al. 2018

Sex Dev

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In clinical practice, it is fundamental to compare the results of hormonal examinations obtained in the laboratory with reliable reference values. This is particularly difficult when faced with rare conditions, such as disorders of sex development, where not routinely assayed peptide hormones as well as intermediate steroid metabolites are often needed and local reliable reference values are not available. There are considerable differences among techniques and assays used in clinical and research laboratories. In fact, laboratory hormonology is undergoing a critical transition between techniques for quantitative determination: established immunoassays and mass spectrometry. Harmonizing results from different laboratories is a major challenge along the path leading to the establishment of consensus reference intervals for steroid hormones. Most of the efforts are being concentrated on testosterone, with very encouraging results being provided by the harmonization of liquid chromatography-tandem mass spectrometry results. However, this goal is still far from being achieved for the other steroid and small-molecule hormones, and a much more challenging perspective is foreseeable for protein hormones. In addition to technical issues, the importance of the definition and of the characterization of the reference population as well as sampling and processing methodology should not be underestimated, as these aspects may impact on hormonal axis and compound stability. The aim of the present review is to provide a comprehensive overview of the circulating reference values in basal condition of the hormones and proteins involved in sex development reported to date in the peer-reviewed literature. We present a series of tables where we have collected the reference intervals for each specific hormone and protein.

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Clinical applications of MS‐based protein quantification

Cited by 32 publications

References 250 publications

Personalized Proteomics: The Future of Precision Medicine

Personalized Proteomics: The Future of Precision Medicine

Proteomics progresses in microbial physiology and clinical antimicrobial therapy

Normative Basal Values of Hormones and Proteins of Gonadal and Adrenal Functions from Birth to Adulthood

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