Clinical Aspects of Nephropathia Epidemica (Puumala Virus Infection) in Europe: A Review

Settergren, Bo

doi:10.1080/003655400750045204

Cited by 86 publications

(69 citation statements)

References 35 publications

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“…Elevated levels of extracellular granzyme B, indicative of the activation of CD8 ϩ T cells and natural killer (NK) cells, have been detected in viral, bacterial, and parasitic infections (9,17,32), and we have previously detected elevated levels of extracellular perforin in human immunodeficiency virus type 1-infected individuals (15). In this study, serum samples were collected from patients hospitalized with laboratory-verified Puumala hantavirus infection and with typical clinical symptoms of acute nephropathia epidemica, a milder form of HFRS (31). The levels of perforin (Mabtech, Nacka, Sweden), granzyme B (Euroclone, Pero, Italy), caspase-cleaved cytokeratin-18 (CK18) (Peviva, Bromma, Sweden), and total CK18 (Peviva) were analyzed using enzymelinked immunosorbent assays according to the manufacturer's instructions.…”

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confidence: 99%

Loss of Cell Membrane Integrity in Puumala Hantavirus-Infected PatientsCorrelates with Levels of Epithelial Cell Apoptosis and Perforin

Klingström

Hardestam

Stoltz

et al. 2006

J Virol

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Hemorrhagic fever with renal syndrome and hantavirus pulmonary syndrome are two diseases caused by hantaviruses. Capillary leakage is a hallmark of hantavirus infection. Pathogenic hantaviruses are not cytotoxic, but elevated levels of serum lactate dehydrogenase (LDH), indicative of cellular damage, are observed in patients. We report increased levels of serum perforin, granzyme B, and the epithelial cell apoptosis marker caspase-cleaved cytokeratin-18 during Puumala hantavirus infection. Significant correlation was observed between the levels of LDH and perforin and the levels of LDH and caspase-cleaved cytokeratin-18, suggesting that tissue damage is due to an immune reaction and that epithelial apoptosis contributed significantly to the damage.

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confidence: 99%

Loss of Cell Membrane Integrity in Puumala Hantavirus-Infected PatientsCorrelates with Levels of Epithelial Cell Apoptosis and Perforin

Klingström

Hardestam

Stoltz

et al. 2006

J Virol

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“…The symptoms of PUUV infection, nephropathia epidemica (NE, a mild form of HFRS), include abrupt fever, followed by headache, nausea, vomiting, abdominal pain, and signs of renal insufficiency; somnolence and visual disturbances are common, and pulmonary, cardiac, and central nervous system symptoms have also been recorded. The incubation period is 2 to 6 weeks (5)(6)(7)(8). Hantavirus infection induces a strong humoral IgM and IgG antibody response against the structural proteins, particularly the nucleocapsid (N) protein in the acute phase.…”

mentioning

confidence: 99%

Rapid Homogeneous Immunoassay Based on Time-Resolved Förster Resonance Energy Transfer for Serodiagnosis of Acute Hantavirus Infection

Hepojoki

Hedman

et al. 2015

J Clin Microbiol

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We recently introduced a homogeneous immunoassay based on time-resolved Förster resonance energy transfer (TR-FRETP uumala virus (PUUV) belongs to the genus Hantavirus within the family Bunyaviridae, comprising typically rodent-and insectivore-borne negative-stranded RNA viruses (1). The known reservoir hosts of hantaviruses are chronically infected and spread the virus via excreta (saliva, urine, and feces) (2). The human infection, acquired via inhalation of aerosolized rodent excreta, manifests as two disease entities: hemorrhagic fever with renal syndrome (HFRS) and hantavirus cardiopulmonary syndrome (HCPS). HFRS predominates in Eurasia, and HCPS occurs in the Americas. The case fatality rate of HFRS is 0.1 to 12%, depending on the virus type, and that of HCPS is up to 40% (3).Annually, around 50,000 hospitalizations due to HFRS occur in Eurasia, while a total of ϳ3,500 HCPS cases have been recorded since 1993 (4). In Europe, the most common cause of HFRS (up to 10,000 cases per annum) is PUUV that circulates in bank voles (Myodes glareolus) (5). The symptoms of PUUV infection, nephropathia epidemica (NE, a mild form of HFRS), include abrupt fever, followed by headache, nausea, vomiting, abdominal pain, and signs of renal insufficiency; somnolence and visual disturbances are common, and pulmonary, cardiac, and central nervous system symptoms have also been recorded. The incubation period is 2 to 6 weeks (5-8). Hantavirus infection induces a strong humoral IgM and IgG antibody response against the structural proteins, particularly the nucleocapsid (N) protein in the acute phase. Laboratory diagnosis of hantavirus disease is based on the detection of IgM and IgG antibodies, usually by immunochromatography (IgM), enzyme immunoassay (EIA) with recombinant N as the antigen, or immunofluorescence assay (IFA) based on acetone-fixed infected cells (9-11).Förster resonance energy transfer (FRET) has been applied widely in biomedical research (12)(13)(14)(15)(16)(17)(18)(19), and our goal is to harness the phenomenon for infectious disease serodiagnostics. In FRET, an excited donor fluorophore transfers its energy to an acceptor fluorophore in close proximity (Ͻ10 nm). Time-resolved FRET (TR-FRET) utilizes the long fluorescence emission half-lives of lanthanides and enables background-free measurement from biological materials. We recently introduced two different principles utilizing TR-FRET for antibody detection. The first approach is based on binding of an immunoglobulin molecule to its antigens labeled distinctly with donor and acceptor fluorophores, giving rise to a FRET signal in a homogenous (wash-free) assay format (12). The second approach is based on binding of an immunoglobulin molecule to its antigen and protein L (a bacterial protein targeting the Ig kappa light chain) labeled distinctly with fluorophores forming a FRET pair (20). The emitted TR-FRET signal is proportional to the amount of specific immunoglobulin. Here, using recombinant full-length N protein of PUUV (PUUV-N), we showed that the protein...

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“…HFRS caused by Hantaan virus (HTNV) and Seoul virus (SEOV) is characterized by its clinical features in various systems, such as neurological, gastrointestinal, and cardiovascular systems with fatality rate up to 12 %. NE is a mild form of HFRS, caused by the Puumala virus (PUUV) with fatality rate of 0.1% up to 0.4% [1].…”

Section: A Hantavirusmentioning

confidence: 99%

Analysis of Hantaviruses Glycoprotein Sequence Using SVM Algorithm

Kim¹,

Jeong²,

Yoon³

2014

IJBBB

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Abstract-Hantaviruses are single-stranded, enveloped, negative sense RNA viruses of the Bunyaviridae family. They induce deadly hemorrhagic fever with fatality up to 40%. Currently, there is no specific cure for Hantaviruses, so more researches about this mortal virus should be done. In order to effectively analyze a variety of different Hantaviruses, we utilize a model called the support vector machine (also known as SVM) which is generally used for analyzing and classifying binary data.The basic mechanism of the SVM is to find the most optimal hyperplane, or the maximum-margin hyperplane, which can separate different types of data with the least error bound. Out of all of the hyperplanes that may be used to classify the data points, the most optimal hyperplane is the one that has the largest margin, or separation among different types of data. In other words, the optimal hyperplane is chosen in the case where the distance between the nearest points of each group of data is maximized. Ultimately, using the selected hyperplane, SVM classifies the data points and computes values such as accuracy and sensitivity. At the end of its operation, the SVM algorithm prints out the computed values. Such SVM algorithms can be used to learn the characteristics of each Hantavirus such as sequence patterns and abundance of amino acids. Since we are the first time to scientifically investigate the Hantavirus with SVM, it is expected that the results of this research will be greatly helpful for further in-depth researching and the development of the cure for the virus.

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Clinical Aspects of Nephropathia Epidemica (Puumala Virus Infection) in Europe: A Review

Cited by 86 publications

References 35 publications

Loss of Cell Membrane Integrity in Puumala Hantavirus-Infected PatientsCorrelates with Levels of Epithelial Cell Apoptosis and Perforin

Loss of Cell Membrane Integrity in Puumala Hantavirus-Infected PatientsCorrelates with Levels of Epithelial Cell Apoptosis and Perforin

Rapid Homogeneous Immunoassay Based on Time-Resolved Förster Resonance Energy Transfer for Serodiagnosis of Acute Hantavirus Infection

Analysis of Hantaviruses Glycoprotein Sequence Using SVM Algorithm

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