Clinical, Bacteriological, and Serological Observations of two Human Volunteers Following Ingestion of Escherichia coli

Wentworth, Frederick H.; Brock, Darrell W.; Stulberg, Cyril S.; Page, R. H.

doi:10.3181/00379727-91-22338

Cited by 27 publications

(9 citation statements)

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“…No animal models existed to demonstrate pathogenicity and no other recognized virulence properties were identifiable by which to differentiate the diarrhea-associated strains from other E. coli. Accordingly, in the early 1950s several groups carried out challenge studies in humans involving oral feeding of EPEC serotypes (95,195,220,221,297,393). Four groups of investigators reported feeding studies in humans with EPEC strains of serogroup 0111; these are summarized in Table 8.…”

Section: Epecmentioning

confidence: 99%

New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development.

Levine¹,

Kaper²,

Black³

et al. 1983

Microbiological Reviews

413

194

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Section: Epecmentioning

confidence: 99%

New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development.

Levine¹,

Kaper²,

Black³

et al. 1983

Microbiological Reviews

413

194

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“…However, data derived from volunteer studies with adults (52,66,105,125,127,213) and from a small number of documented outbreaks among adults show that disease does occur but the infective inoculum is high (126). The reason for the marked resistance of adults to infection is not clear; the antibody response to the O antigen of certain 0 serogroups is age related, and Neter et al (149) proposed that exposure to EPEC during childhood conferred immunity in later life.…”

Section: Current Aspects Of Epec Infectionmentioning

confidence: 99%

Adhesion and its role in the virulence of enteropathogenic Escherichia coli

Law

1994

Clin Microbiol Rev

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Enteropathogenic Escherichia coli (EPEC) organisms are an important cause of diarrheal disease in young children. The virulence of EPEC is a multifactorial process and involves a number of distinct stages. Initial adherence to intestinal mucosa is mediated by fimbriae which bring about a distinct form of adhesion, localized adhesion. Intimate adhesion of the bacterium to the eukaryotic membrane occurs, resulting in the activation of signal transduction pathways. Microvilli are disrupted and effaced from the apical membrane which then cups around the organism to form pedestal structures, the attaching and effacing lesion. Diarrhea may be produced by alteration of the permeability of the apical membrane and also through a malabsorption mechanism. The pathways involved in the production of the attaching and effacing lesion are described. EPEC organisms were originally thought to belong to a number of distinct serogroups; it is now apparent that many isolates belonging to these serogroups are not pathogenic or belong to other pathogenic groups of E. coli. In addition, isolates falling outside of these serogroups are considered to be true EPEC. The definition of EPEC based on serotyping is inaccurate and should be replaced by methods that specifically detect the virulence properties of EPEC.

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“…antisera [6][7][8]. Although no biochemical, microbiological or animal-model assays at that time could demonstrate an inherent pathogenicity vis-a-vis other E. coli, experimental challenge studies in the early 1950s in the United States, England, and Japan confirmed that strains of serogroups 055, 0111, and 0127, isolated from infants with gastroenteritis, caused diarrhea when fed to volunteers [9][10][11][12][13][14]. Also at this time, Ewing et al [6,8,15] in the United States and Taylor [7] in England noted that among all the EPEC, certain 0 serogroups, including 026, 055, 0111, 0119, 0127, and 0128, were particularly frequent and epidemiologically well-incriminated in association with diarrhea, whereas isolates of other EPEC 0 serogroups, such as 044, 086, and 0114, were less common.…”

mentioning

confidence: 99%

The Diarrheal Response of Humans to Some Classic Serotypes of Enteropathogenic Escherichia coli is Dependent on a Plasmid Encoding an Enteroadhesiveness Factor

Levine

Nataro

Karch

et al. 1985

Journal of Infectious Diseases

440

299

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Isolates of the most common O serogroups of enteropathogenic Escherichia coli (EPEC) associated with infant diarrhea (designated class I) adhere to Hep-2 cells; the genes for this adhesin, termed EPEC adherence factor (EAF), are located on plasmids 50-70 MDa in size. Volunteers ingested 10(10) organisms of an O127:H6 Hep-2-adhesive class I strain (E2348/69) or its plasmid-minus, nonadhesive derivative. Diarrhea occurred in nine of 10 volunteers who ingested the parent strain (mean, 1,178 ml) but in only two of nine who took the plasmid-minus variant (mean, 433 ml; P less than .006). All volunteers ill from strain E2348/69 mounted serum IgA and IgG responses to a 94-kDa plasmid-associated outer membrane protein of E2348/69; this protein was found in other class I EPEC but not in enterotoxigenic or meningitic strains. The 50-70-MDa EAF plasmid seems necessary for full expression of pathogenicity in EPEC that exhibit Hep-2 adhesiveness. EPEC isolates of certain other, less common, O serogroups (O44, O86, and O114) are rarely Hep-2 adhesive. These EPEC, designated class II, possess distinct 50-70 MDa plasmids lacking EAF genes. Diarrhea was caused by 10(8) or 10(10) organisms of an O114:H2 class II EPEC strain (mean, 1,156 ml) in six of 11 volunteers. This result confirmed that class II EPEC are pathogenic by a mechanism not involving Hep-2 adhesiveness.

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Clinical, Bacteriological, and Serological Observations of two Human Volunteers Following Ingestion of Escherichia coli

Cited by 27 publications

References 0 publications

New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development.

New knowledge on pathogenesis of bacterial enteric infections as applied to vaccine development.

Adhesion and its role in the virulence of enteropathogenic Escherichia coli

The Diarrheal Response of Humans to Some Classic Serotypes of Enteropathogenic Escherichia coli is Dependent on a Plasmid Encoding an Enteroadhesiveness Factor

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