Clinical Chemistry, Molecular Diagnostics, and Laboratory Medicine

Bruns, David E.; Ashwood, Edward R.; Burtis, C. A.

doi:10.1016/b978-1-4160-6164-9.00001-9

Cited by 56 publications

(124 citation statements)

References 3 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…There are about 78 000 people sent to the emergency room, 33 000 hospitalizations, and 150 deaths because of AP overdose every year. 2–4 AP hepatotoxicity does not result from AP itself but its metabolites. AP is converted by the drug metabolizing enzymes to reactive metabolites, mainly N-acetyl-p-benzoquinone imine (NAPQI), which can occur in a complex mechanistic sequence by rapidly depleting the glutathione and covalently bonding to nucleophilic aspects of the cell.…”

mentioning

confidence: 99%

Point-of-Care Determination of Acetaminophen Levels with Multi-Hydrogen Bond Manipulated Single-Molecule Recognition (eMuHSiR)

et al. 2018

View full text Add to dashboard Cite

This work aims to face the challenge of monitoring small molecule drugs accurately and rapidly for point-of-care (POC) diagnosis in current clinical settings. Overdose of acetaminophen (AP), a commonly used over the counter (OTC) analgesic drug, has been determined to be a major cause of acute liver failure in the US and the UK. However, there is no rapid and accurate detection method available for this drug in the emergency room. The present study examined an AP sensing strategy that relies on a previously unexplored strong interaction between AP and the arginine (Arg) molecule. It was found that as many as 4 hydrogen bonds can be formed between one Arg molecule and one AP molecule. By taking advantages of this structural selectivity and high tenability of hydrogen bonds, Arg, immobilized on a graphene surface via electrostatic interactions, was utilized to structurally capture AP. Interestingly, bonded AP still remained the perfect electrochemical activities. The extent of Arg-AP bonds was quantified using a newly designed electrochemical (EC) sensor. To verify the feasibility of this novel assay, based on multihydrogen bond manipulated single-molecule recognition (eMuHSiR), both pharmaceutical and serum sample were examined. In commercial tablet measurement, no significant difference was seen between the results of eMuHSiR and other standard methods. For measuring AP concentration in the mice blood, the substances in serum, such as sugars and fats, would not bring any interference to the eMuHSiR in a wide concentration range. This eMuHSiR method opens the way for future development of small molecule detection for the POC testing.

show abstract

mentioning

confidence: 99%

Point-of-Care Determination of Acetaminophen Levels with Multi-Hydrogen Bond Manipulated Single-Molecule Recognition (eMuHSiR)

et al. 2018

View full text Add to dashboard Cite

show abstract

“…Thus, outliers (defined as values outside of the first quartile minus 1.5 multiplied by IQR and values outside of the third quartile plus 1.5 multiplied by IQR) were removed. The 2.5 and 97.5 percentiles were defined using 4 different models: (1) no exclusions, (2) outliers removed according to the Tietz method, 36 (3) individuals with exclusion criteria removed, and (4) both outliers and individuals with exclusion criteria removed. The exclusion criteria used in models 3 and 4 were defined as any record of preeclampsia, maternal diabetes, maternal kidney disease, congenital kidney deformity (diagnosis codes are available in eTable 1 in the Supplement), maternal BMI greater than 35, and SGA at birth.…”

Section: Discussionmentioning

confidence: 99%

“…To examine the association of maternal comorbidity with creatinine and urea concentrations in umbilical cord blood, odds ratios (ORs) were calculated using a logistic regression analysis with cutoffs corresponding to the upper reference interval limit (0.98 mg/dL for creatinine and 14.29 mg/dL for urea) (eTable 10 and eTable 11 in the Supplement). The models included umbilical cord blood samples from newborns of all GAs; however, a sensitivity analysis including only those born at 36 Exclusion criteria were preeclampsia, maternal diabetes, maternal kidney disease, congenital kidney deformity, maternal body mass index (calculated as weight in kilograms divided by height in meters squared) greater than 35, and small for GA at birth. Newborn reference intervals from the time of birth were stratified by GA.…”

Section: Maternal Comorbidity and Risk Of High Concentrations Of Crea...mentioning

confidence: 99%

Markers of Kidney Function in Early Childhood and Association With Maternal Comorbidity

et al. 2022

View full text Add to dashboard Cite

ImportanceKidney functional capacity is low at birth but doubles during the first 2 weeks of life and reaches near-adult levels at age 1 to 2 years. Existing reference intervals for markers of kidney function in newborns are mostly based on preterm newborns, newborns with illness, or small cohorts of term newborns, and the consequences of maternal comorbidities for newborn kidney function are sparsely described.ObjectiveTo establish robust reference intervals for creatinine and urea in healthy children in early childhood and to assess whether maternal comorbidity is associated with newborn creatinine and urea concentrations.Design, Setting, and ParticipantsThis multicenter, prospective, population-based cohort study assessed data and umbilical cord blood samples from participants in the Copenhagen Baby Heart Study (CBHS) who were born between April 1, 2016, and October 31, 2018, and venous blood samples from a subsample of CBHS participants who were enrolled in the COMPARE study between May 3, 2017, and November 4, 2018. Cord blood samples of 13 354 newborns from the CBHS and corresponding venous blood samples of 444 of those newborns from the COMPARE study were included. Blood samples were collected at birth, age 2 months, and age 14 to 16 months, with follow-up completed on February 12, 2020. Healthy nonadmitted term newborns from maternity wards at 3 hospitals in the Capital Region of Denmark were included.ExposuresMaternal comorbidity.Main Outcomes and MeasuresCreatinine and urea concentrations.ResultsAmong 13 354 newborns in the CBHS cohort, characteristics of 12 938 children were stratified by sex and gestational age (GA). Of those, 6567 children (50.8%) were male; 5259 children (40.6%) were born at 37 to 39 weeks’ GA, and 7679 children (59.4%) were born at 40 to 42 weeks’ GA. Compared with children born at 40 to 42 weeks’ GA, those born at 37 to 39 weeks’ GA had lower birth weight, Apgar scores at 5 minutes, placental weight, and placental-fetal weight ratio. Children born at 37 to 39 weeks’ GA vs those born at 40 to 42 weeks’ GA were more frequently small for GA at birth and more likely to have placental insufficiency and exposure to maternal preeclampsia, maternal diabetes, maternal kidney disease, and maternal hypertension. Among children born at 37 to 39 weeks’ GA, reference intervals were 0.54 to 1.08 mg/dL for creatinine and 5.32 to 14.67 mg/dL for urea; among children born at 40 to 42 weeks’ GA, reference intervals were 0.57 to 1.19 mg/dL for creatinine and 5.60 to 14.85 mg/dL for urea. At birth, multifactorially adjusted odds ratios among children exposed to preeclampsia were 9.40 (95% CI, 1.68-52.54) for a venous creatinine concentration higher than the upper reference limit, 4.29 (95% CI, 1.32-13.93) for a venous creatinine concentration higher than the 90th percentile, and 3.10 (95% CI, 1.14-8.46) for a venous creatinine concentration higher than the 80th percentile.Conclusions and RelevanceIn this study, improved reference intervals for creatinine and urea concentrations were generated. Preeclampsia was associated with an increased risk of high newborn creatinine concentrations, suggesting that newborns of mothers with preeclampsia need closer observation of their kidney function.

show abstract

“…Ceruloplasmin (Cp) is an α2-globulin that contains 95% of the total copper found in serum. [5] Each molecule of Cp contains six to eight copper atoms, most of which are tightly bound. [6] Cp is synthesized primarily by the hepatic parenchymal cells.…”

Section: Discussionmentioning

confidence: 99%

“…Once thought to be under control, TB is now the number one cause of infection related death world-wide. [5] 95% of the TB infection incidence is in developing countries. [10,11] India has the highest TB burden in terms of absolute number of incidence.…”

Section: Discussionmentioning

confidence: 99%

Serum Ferroxidase Activity of Ceruloplasmin in Pulmonary Tuberculosis

Anuradha¹,

Pasupuleti²

2015

JCBS

View full text Add to dashboard Cite

The objective of this study is to incorporate serum Ferroxidase as a surrogate marker of pulmonary tuberculosis (PTB) Fifty patients with PTB whose sputum samples were positive for acid fast bacilli (Group I) and 50 subject who were completely treated for PTB (Group II) were compared with 50 healthy controls. The difference, were similarly found between male and female Group I subject and Controls in serem ferroxidase levels. Serum Ferroxidase was measured by end point method of Ozcan Erel at 600nm. Mean ± SD of serum Ferroxidase in control, Group I and Group II were 800.87±130IU/L, 1616.02±216 IU/L and 851.12±201 IU/L respectively. Serum Ferroxidase in Group I was significantly higher as compared to control (P<0.001) whereas in Group II it was not significant when compared with control. Serum Ferroxidase which was higher in newly diagnosed PTB patients falls back to normal level on complete treatment with ATT. Hence serum Ferroxidase activity of ceruloplasmin can be used both as a diagnostic and prognostic marker of PTB.

show abstract

Clinical Chemistry, Molecular Diagnostics, and Laboratory Medicine

Cited by 56 publications

References 3 publications

Point-of-Care Determination of Acetaminophen Levels with Multi-Hydrogen Bond Manipulated Single-Molecule Recognition (eMuHSiR)

Point-of-Care Determination of Acetaminophen Levels with Multi-Hydrogen Bond Manipulated Single-Molecule Recognition (eMuHSiR)

Markers of Kidney Function in Early Childhood and Association With Maternal Comorbidity

Serum Ferroxidase Activity of Ceruloplasmin in Pulmonary Tuberculosis

Contact Info

Product

Resources

About