Clinical Comparison of the Effectiveness of Single-file Reciprocating Systems and Rotary Systems for Removal of Endotoxins and Cultivable Bacteria from Primarily Infected Root Canals

Martinho, Frederico Canato; Gomes, Ana Paula Martins; Fernandes, Antônio Carlos; Ferreira, Nádia; Endo, Marcos Sérgio; Freitas, Lílian Ferreira; Camões, Izabel Coelho Gomes

doi:10.1016/j.joen.2013.12.006

Cited by 61 publications

(100 citation statements)

References 35 publications

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“…24 The only techniques that have been tested so far and have showed a certain degree of effectiveness against LPS are (a) the use of calcium hydroxide as an intra-canal medication 12,25,26 as it hydrolyses lipid A and changes it into chains of fatty acid and nontoxic sugars, 27 and (b) the application of laser Nd:YAG. 9 The results obtained in this study reinforce the importance of instrumentation in addition to any disinfection protocol, 11,28,29 especially with regard to bacterial LPS that strongly adheres to the dentinal wall 6 and makes it necessary to use endodontic instruments for its removal. Some studies have reported endotoxin decrease up to 44.4%, 30 59.99%, 28 and 57.98% 10 after chemo-mechanical preparation.…”

Section: Discussionsupporting

confidence: 67%

“…Furthermore, this approach allows for deeper irrigation and increases the probability of opening secondary canals and apical deltas. However, this was contradicted by Martinho et al 29 who tested different systems with different designs, conicities, and tapers such as WaveOne ® , Reciproc ® , Protaper ® , and Mtwo ® and did not observe any statistically significant differences in the decrease of bacterial LPS. The percentage of LPS reduction observed with each system was 95.15%, 96.21%, 97.98%, and 96.34%, respectively.…”

Section: Discussionmentioning

confidence: 86%

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LPS levels in root canals after the use of ozone gas and high frequency electrical pulses

et al. 2016

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The present study aims to verify the effect of ozone gas (OZY ® System) and high frequency electric pulse (Endox ® System) systems on human root canals previously contaminated with Escherichia coli lipopolysaccharide (LPS). Fifty single-rooted teeth had their dental crowns removed and root lengths standardized to 16 mm. The root canals were prepared up to #60 hand K-files and sterilized using gamma radiation with cobalt 60. The specimens were divided into the following five groups (n = 10) based on the disinfection protocol used: OZY ® System, one 120-second-pulse (OZY 1p); OZY ® System, four 24-second-pulses (OZY 4p); and Endox ® System (ENDOX). Contaminated and non-contaminated canals were exposed only to apyrogenic water and used as positive (C+) and negative (C-) controls, respectively. LPS (O55:B55) was administered in all root canals except those belonging to group C-. After performing disinfection, LPS samples were collected from the canals using apyrogenic paper tips. Limulus Amoebocyte Lysate (LAL) was used to quantify the LPS levels, and the data obtained was analyzed using one-way ANOVA. The disinfection protocols used were unable to reduce the LPS levels significantly (p = 0.019). The use of ozone gas and high frequency electric pulses was not effective in eliminating LPS from the root canals.

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Section: Discussionsupporting

confidence: 67%

Section: Discussionmentioning

confidence: 86%

LPS levels in root canals after the use of ozone gas and high frequency electrical pulses

et al. 2016

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“…Rezultati ovog istraživanja nisu u skladu s rezultatima istraživanja Alvesa i suradnika (10), Basmacija, Oztana i Kiyana (11), Martinha i njegovih kolega (14), Machada i njegova tima (15) te Siquere i suradnika. (16).…”

Section: Discussionunclassified

“…Iako se nekoliko dosadašnjih istraživanja fokusiralo na učinkovitost čišćenja (12), apikalno izbacivanje otpada (13) te na redukciju endotoksina (14) jednoigličnog sistema, u nekoliko studija (10,11,15,16) analizirana je mogućnost smanjivanja broja mikroorganizama u kontaktu s antimikrobnom otopinom za ispiranje.…”

Section: Uvodunclassified

“…Thus, although several previous studies have evaluated the cleaning efficacy (12), the apical extrusion of debris (13), and the reduction of endotoxins (14) of single-file systems, only few studies (10,11,15,16) have analyzed the ability to reduce intracanal microbial agents when associated with antimicrobial irrigating solutions.…”

Section: Odabir I Priprema Uzorkamentioning

confidence: 99%

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