Selective injection of 1.5 ml of 10% saline into the left circumflex coronary artery (LCX) was carried out during sinus rhythm twice in each of 15 mongrel dogs anesthetized with sodium pentobarbital, and multiple endocardial recordings including His bundle electrogram (HBE) were made. Ventricular tachycardia (VT) was elicited in all of the 15 dogs both in the first and second injections. The cycle length of sinus rhythm before injection was 468+88 msec (mean+s.D.). The coupling interval between the last depolarization of the left ventricle (LV) of sinus origin and the first premature LV depolarization of the VT was 395+57 msec. Coupling interval between the first and the second LV depolarizations was 348+78 msec. Significant difference was noted between any pair of these values (p<0.01). No bundle branch re-entry in the beginning of VT was observed. The site of origin of the VT was referred to the LV in 13 dogs. Rapid pacing during paroxysm of VT applied at the apex of the LV made no marked influence upon VT after pacing. The calcium channel blocker, diltiazem failed to block the production of VT. The mechanism for genesis of this VT may not be re-entry but rather enhanced automaticity independent of the calcium inward current. --ventricular tachycardia; intracoronary injection; endocardial electrography The incidence of VT per se is not quite high, although many methods for experimental production of VT in animal study have been reported (Siebecker et al. 1961;Scherf and Bornemann 1967;Wallace et al. 1967;Bellet 1971;Lown et al. 1977) and ventricular tachyarrhythmias including VT can be induced by these methods. In the previous reports (Abe 1979(Abe , 1980) the author demonstrated with surface electrocardiography (ECG) that VT could be successfully elicited by intracoronary injections of hyperosmotic solutions in canine hearts with 100% incidence. In the present experiments VT produced by intracoronary hyperosmotic saline was studied with endocardial electrography.
METHODSFifteen mongrel dogs weighing between 20 and 29 kg (average, 24.6 kg) were anesthetized with intramuscular sodium pentobarbital (35 mg/kg), incubated with a cuffed