This study investigated hydrogen peroxide (H 2 O 2 ) concentration of two home bleaching gels, in their dispensing syringes and their degradation in vivo in intraoral bleaching trays. Two bleaching gels were studied, 7.5% hydrogen peroxide (HP) and 20% carbamide peroxide (CP). The concentration of H 2 O 2 was determined in dispensing syringes. Twenty individuals were involved in this study. The gels were placed in trays of both arches and their degradation determined at 5, 10, 15, 20, 30, 40, 60 and 75 min. The concentrations of H 2 O 2 in syringes were (HP) 8.12% and (CP) 7.95%. For the HP gel in custom-trays the concentration of H 2 O 2 was 73% at 5 min and 42% after 75 min. In the 20% CP gel it was 75% at 5 min and 39% after 75 min. Activity decreased linearly up to 75 min, where the mean concentration of H 2 O 2 exceeded 35% for the CP and HP gels.