Clinical efflux of cryoprotective agents from vitrified human articular cartilage

Yu, Hana; Al-Abbasi, Khaled K.; Elliott, Janet A.W.; McGann, Locksley E.; Jomha, Nadr M.

doi:10.1016/j.cryobiol.2012.12.005

Cited by 12 publications

(1 citation statement)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Our group has been focused on vitrification as a means of long-term AC storage. [14][15][16][17][18][19][20] Vitrification uses high concentrations of cryoprotective agents (CPAs) and rapid cooling to preserve cells or tissues in a non-crystalline, amorphous solid that is in a glassy state at −196 °C. 15,21 However, the use of high CPA concentrations and rapid cooling in vitrification causes various cellular stresses such as osmotic stress, chilling injury, oxidative stress, and CPA toxicity.…”

Section: Introductionmentioning

confidence: 99%

Chondrocyte Viability of Particulated Porcine Articular Cartilage Is Maintained in Tissue Storage After Cryoprotectant Exposure, Vitrification, and Tissue Warming

Crisol

Congdon

et al. 2023

CARTILAGE

Self Cite

View full text Add to dashboard Cite

Objective Vitrification of articular cartilage (AC) is a promising technique which may enable long-term tissue banking of AC allografts. We previously developed a 2-step, dual-temperature, multi-cryoprotectant agent (CPA) loading protocol to cryopreserve particulated AC (1 mm3 cubes). Furthermore, we also determined that the inclusion of ascorbic acid (AA) effectively mitigates CPA toxicity in cryopreserved AC. Prior to clinical translation, chondrocytes must remain viable after tissue re-warming and before transplantation. However, the effects of short-term hypothermic storage of particulated AC after vitrification and re-warming are not documented. This study evaluated the chondrocyte viability of post-vitrified particulated AC during a 7-day tissue storage period at 4 °C. We hypothesized that porcine particulated AC could be stored for up to 7 days after successful vitrification without significant loss of cell viability, and these results would be enhanced when cartilage is incubated in storage medium supplemented with clinical grade AA. Design Three experimental groups were examined at 5 time points: a fresh control (only incubated in medium), a vitrified − AA group, and a vitrified + AA group ( N = 7). Results There was a mild decline in cell viability but both treatment groups maintained a viability of greater than 80% viable cells which is acceptable for clinical translation. Conclusion We determined that particulated AC can be stored for up to 7 days after successful vitrification without a clinically significant decline in chondrocyte viability. This information can be used to guide tissue banks regarding the implementation of AC vitrification to increase cartilage allograft availability.

show abstract