Clinical evaluation of a disposable amperometric magneto-genosensor for the detection and identification of Streptococcus pneumoniae

Sotillo, Alma; Pedrero, Marı́a; Pablos, Manuela de; Garcı́a, José Luis; Garcı́a, Ernesto; Garcı́a, Pedro; Pingarrón, José M.; Mingorance, Jesús; Campuzano, Susana

doi:10.1016/j.mimet.2014.04.014

Cited by 18 publications

(16 citation statements)

References 18 publications

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“…In particular, genosensors based on magnetic microbeads and PCR amplification, have been broadly used in clinical applications and food analysis because of their excellent sensitivity, reproducibility, reduced time of analysis, high sample throughput and easy automation with minimum consumption of the sample and reagents [39,40]. To the best of our knowledge, only a few genosensors, mainly microarrays, have been developed for fungal pathogen screening and identification.…”

Section: Introductionmentioning

confidence: 99%

Species-specific optical genosensors for the detection of mycotoxigenic Fusarium fungi in food samples

Peltomaa

Vaghini

Patiño

et al. 2016

Analytica Chimica Acta

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Section: Introductionmentioning

confidence: 99%

Species-specific optical genosensors for the detection of mycotoxigenic Fusarium fungi in food samples

Peltomaa

Vaghini

Patiño

et al. 2016

Analytica Chimica Acta

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“…At this point, it is worth to mention that very recently the Lobo-Castañón’s research group has reported, for the first time, the use of on-surface helicase-dependent amplification at ITO surfaces to quantify electrochemically very small amounts of genomic DNA extracted from Salmonella [ 31 ]. Attractive electrochemical nucleic acid sensors have been reported so far for non-invasive bacterial infections detection coupled with PCR [ 32 , 33 , 34 ], HDA [ 25 , 31 , 34 ], RPA [ 35 ] and HCR [ 36 ].…”

Section: Electrochemical Affinity Biosensing Of Infectious Pathogementioning

confidence: 99%

Molecular Biosensors for Electrochemical Detection of Infectious Pathogens in Liquid Biopsies: Current Trends and Challenges

Campuzano

Yáñez‐Sedeño

Pingarrón

2017

Sensors

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Rapid and reliable diagnosis of infectious diseases caused by pathogens, and timely initiation of appropriate treatment are critical determinants to promote optimal clinical outcomes and general public health. Conventional in vitro diagnostics for infectious diseases are time-consuming and require centralized laboratories, experienced personnel and bulky equipment. Recent advances in electrochemical affinity biosensors have demonstrated to surpass conventional standards in regards to time, simplicity, accuracy and cost in this field. The tremendous potential offered by electrochemical affinity biosensors to detect on-site infectious pathogens at clinically relevant levels in scarcely treated body fluids is clearly stated in this review. The development and application of selected examples using different specific receptors, assay formats and electrochemical approaches focusing on the determination of specific circulating biomarkers of different molecular (genetic, regulatory and functional) levels associated with bacterial and viral pathogens are critically discussed. Existing challenges still to be addressed and future directions in this rapidly advancing and highly interesting field are also briefly pointed out.

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“…Furthermore, this methodology demonstrated a clear discrimination against Streptococcus mitis (a closely related streptococcus) and between blood and urine samples non-inoculated and inoculated with the target bacteria at a very low concentration (10 3 CFUs mL −1 ). The DNA sensor was successfully validated with 109 clinical samples of diverse origins providing both sensitivity and specificity of around 90% [55]. …”

Section: Electrochemical Genosensing Of Circulating Biomarkersmentioning

confidence: 99%

Electrochemical Genosensing of Circulating Biomarkers

Campuzano

Yáñez‐Sedeño

Pingarrón

2017

Sensors

Self Cite

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Management and prognosis of diseases requires the measurement in non- or minimally invasively collected samples of specific circulating biomarkers, consisting of any measurable or observable factors in patients that indicate normal or disease-related biological processes or responses to therapy. Therefore, on-site, fast and accurate determination of these low abundance circulating biomarkers in scarcely treated body fluids is of great interest for health monitoring and biological applications. In this field, electrochemical DNA sensors (or genosensors) have demonstrated to be interesting alternatives to more complex conventional strategies. Currently, electrochemical genosensors are considered very promising analytical tools for this purpose due to their fast response, low cost, high sensitivity, compatibility with microfabrication technology and simple operation mode which makes them compatible with point-of-care (POC) testing. In this review, the relevance and current challenges of the determination of circulating biomarkers related to relevant diseases (cancer, bacterial and viral infections and neurodegenerative diseases) are briefly discussed. An overview of the electrochemical nucleic acid–based strategies developed in the last five years for this purpose is given to show to both familiar and non-expert readers the great potential of these methodologies for circulating biomarker determination. After highlighting the main features of the reported electrochemical genosensing strategies through the critical discussion of selected examples, a conclusions section points out the still existing challenges and future directions in this field.

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Clinical evaluation of a disposable amperometric magneto-genosensor for the detection and identification of Streptococcus pneumoniae

Cited by 18 publications

References 18 publications

Species-specific optical genosensors for the detection of mycotoxigenic Fusarium fungi in food samples

Species-specific optical genosensors for the detection of mycotoxigenic Fusarium fungi in food samples

Molecular Biosensors for Electrochemical Detection of Infectious Pathogens in Liquid Biopsies: Current Trends and Challenges

Electrochemical Genosensing of Circulating Biomarkers

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