Clinical evaluation of antimicrobial peptide [99mTc/Tricine/HYNIC0]ubiquicidin 29–41 as a human-specific infection imaging agent

Ghanei, Mostafa; Najafi, Rezvan; Shafiei, Mohammad; Mazidi, Mohammad; Goudarzi, Mostafa; Mirfallah, Seyed Hassan; Ebrahimi, Farhad; Heydarpor, Hamid Reza; Abdie, Noroz

doi:10.1016/j.nucmedbio.2008.11.003

Cited by 53 publications

(48 citation statements)

References 15 publications

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“…In this (Fig. 2b) and previous human studies, 99m Tc-UBI 29-41 scans expressed rapid concentration to the target region and fast renal clearance with negligible liver uptake and hepatobiliary excretion [14,22,31].…”

Section: Resultssupporting

confidence: 83%

“…In this study, the maximum contrast (A/N ratio) was obtained 15 min after 99m Tc-UBI administrations, as previously mentioned [14,22]. These data may suggest a first pass-like distribution with strong avidity of the tracer in the direction of its targets, facilitating image interpretation to distinguish infection as early as 15 min postinjection.…”

Section: Resultssupporting

confidence: 79%

“…In other studies conducted by Melendez-Alafort et al [31] and Becker and Meller [18], 100% sensitivity and specificity were also shown, whereas in another study by Akhtar et al [22], the sensitivity and specificity values were 100 and 80%, respectively. In a small study, in seven patients with the same method of preparation and labeling, 99m Tc-UBI also showed 100% accuracy in osteomyelitis detection [14]. Recently, in a study of 196 patients with fever of unknown origin, specificity for discrimination between sterile inflammation and infection was 95.35%, sensitivity was 97.52%, and accuracy was 96.62% [15].…”

Section: Resultsmentioning

confidence: 97%

“…Preparation of 99m Tc-UBI entailed adding 740 MBq (20 mCi) 99m Tc to vials of HYNIC-UBI 29-41 from a kit [14] (Radioisotope Division, Atomic Energy Organization of Iran) and incubating for 15 min at room temperature. The average radiochemical purity of 99m Tc-HYNIC-UBI 29-41, produced by lyophilized kits, was more than 95%, congruent with a specific activity of 67.6 GBq/mmol, obtained by high-performance liquid chromatography, instant thin-layer chromatography, and solid-phase extraction.…”

Section: Participantsmentioning

confidence: 99%

“…Furthermore, 99m Tc-UBI 29-41 has been used to monitor the effectiveness of treatment of experimental infections in animals [13]. Although results from limited preclinical and pilot studies in patients are promising [14,15], this important agent is not currently available for routine clinical use. The aim of this study was to compare the sensitivity and specificity of 99m Tclabeled UBI scintigraphy with bone scans in the diagnosis of osteomyelitis.…”

Section: Introductionmentioning

confidence: 99%

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Diagnostic value of 99mTc-ubiquicidin scintigraphy for osteomyelitis and comparisons with 99mTc-methylene diphosphonate scintigraphy and magnetic resonance imaging

Assadi

Vahdat²,

Nabipour³

et al. 2011

Nuclear Medicine Communications

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Objective The discrimination of bacterial infections from sterile inflammatory processes is of great importance in the management of inflammation. Currently available techniques cannot decisively address this issue. In this respect, antimicrobial peptide 99m Tc-ubiquicidin (UBI) 29-41 scans have been showing interesting results. The aim of this study was to determine the accuracy of 99m Tc-UBI scan in the detection of osteomyelitis and to compare it with 99m Tc-methylene diphosphonate scan and magnetic resonance imaging (MRI).Methods Twenty patients (mean age = 48.90 years) with suspected osteomyelitis were included in this study. After evaluation of each patient through history taking, physical examination, appropriate laboratory tests, and other processes including bone probing, wound culture, and plain film radiography, MRIs, 99m Tc-UBI scans, and 99m Tc-methylene diphosphonate scans were performed. For quantitative analysis, the mean count of abnormal-tonormal (A/N) region was calculated for images acquired at 15, 30, 45, 60, 120, and 240 min to obtain the most favorable time for imaging. Results In total, osteomyelitis was detected in the 99mTc-UBI scans of 17 patients, indicating 100% accuracy, compared with an accuracy of 90% for osteomyelitis detected in three-phase bone scans. The maximum mean A/N was observed at 15 min after intravenous injection (median: 1.91; interquartile range: 1.54-2.94). MRI was performed in 12 cases only with 75% accuracy. In addition, the A/N ratios for the 99m Tc-UBI scans were not significantly different between patients with or without Staphylococcus aureus growth on wound cultures.Conclusion For fast imaging with high accuracy, 99m Tc-UBI 29-41 is a suitable choice for the detection of osteomyelitis.

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Section: Resultssupporting

confidence: 83%

Section: Resultssupporting

confidence: 79%

Section: Resultsmentioning

confidence: 97%

Section: Participantsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Diagnostic value of 99mTc-ubiquicidin scintigraphy for osteomyelitis and comparisons with 99mTc-methylene diphosphonate scintigraphy and magnetic resonance imaging

Assadi

Vahdat²,

Nabipour³

et al. 2011

Nuclear Medicine Communications

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^99m Tc Radiopharmaceutical Chemistry

Papagiannopoulou¹

2020

Handbook of Radiopharmaceuticals

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Photodynamic eradication of intratumoral microbiota with bacteria‐targeted micelles overcomes gemcitabine resistance of pancreatic cancer

Liu,

Yang,

et al. 2023

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Increasing evidence suggests that intratumoral microbiota plays a pivotal role in tumor progression, immunosurveillance, metastasis, and chemosensitivity. Particularly, in pancreatic ductal adenocarcinoma, tumor‐resident Gammaproteobacteria could transform the chemotherapeutic drug gemcitabine (Gem) into its inactive form, thus rendering chemotherapy ineffective. Herein, a strategy for selectively eradicating intratumoral bacteria was described for overcoming Gem resistance in a pancreatic cancer animal model. An antimicrobial peptide was linked with photosensitizer through a poly (ethylene glycol) chain, which can self‐assemble into micelles with a diameter of ∼20 nm. The micelles could efficiently kill bacteria under light irradiation by inducing membrane depolarization, thereby inhibiting Gem metabolism. In a bacteria‐resident pancreatic cancer animal model, the selective photodynamic eradication of intratumoral bacteria was demonstrated to efficiently reverse Gem resistance. This research highlights antibacterial photodynamic therapy as a promising adjuvant strategy for cancer therapy by modulating intratumoral microbiota.

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Clinical evaluation of antimicrobial peptide [99mTc/Tricine/HYNIC0]ubiquicidin 29–41 as a human-specific infection imaging agent

Cited by 53 publications

References 15 publications

Diagnostic value of 99mTc-ubiquicidin scintigraphy for osteomyelitis and comparisons with 99mTc-methylene diphosphonate scintigraphy and magnetic resonance imaging

Diagnostic value of 99mTc-ubiquicidin scintigraphy for osteomyelitis and comparisons with 99mTc-methylene diphosphonate scintigraphy and magnetic resonance imaging

^99m Tc Radiopharmaceutical Chemistry

Photodynamic eradication of intratumoral microbiota with bacteria‐targeted micelles overcomes gemcitabine resistance of pancreatic cancer

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