Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virus

Cho, Yoon‐Kyoung; Kim, Jintae; Lee, Young Sun; Kim, Young-A; Namkoong, Kak; Lim, Hee-Kyun; Oh, Kwang W.; Kim, Suhyeon; Han, Jungim; Park, Chinsung; Pak, Yong-Ju; Ki, Chang‐Seok; Choi, Jong Rak; Myeong, Hyeon-Koon; Ko, Cheng−Hao

doi:10.1016/j.bios.2005.10.005

Cited by 73 publications

(58 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Several typical results are compared and listed in Table 1. In general, solid covers can be used to seal the liquid sample to form a close microfluidic system, where liquid sample evaporation is greatly reduced and no appreciable evaporation loss can be observed over a long period (Cho et al 2006;Moerman et al 2005). The principle of this approach is to resist the internal pressure generated during thermal reactions.…”

Section: Comparison Of Different Methods To Decrease/ Prevent Liquid mentioning

confidence: 99%

“…Another approach is to use microvalves or solid lids to seal the reaction chamber (Moerman et al 2005;Cheng et al 2005;Cho et al 2006;Oh et al 2005;Pal et al 2005;Pilarski et al 2005;Prakash et al 2006;Toriello et al 2006Toriello et al , 2008. It is common knowledge that the evaporation rate reduces with increase of the gas pressure around a liquid.…”

Section: Introductionmentioning

confidence: 98%

“…A bioreaction microsystem offers several advantages, including reduced reagent costs and the amount of chemical waste, decreased reaction time, reduced risk of contamination, simplified liquid handling, and high integration. In general, there are two kinds of miniaturized PCR systems , a steady fluid PCR (Cho et al 2006;Easley et al 2006;Guttenberg et al 2005;Legendre et al 2006;Matsubara et al 2005;Neuzil et al 2006;Oh et al 2005;Pal et al 2005;Pilarski et al 2005;Prakash et al 2006;Toriello et al 2008), and a dynamic flow PCR (Cheng et al 2005;Hashimoto et al 2006;Kopp et al 1998;Sista et al 2008). For these two categories of miniaturized systems, recent trends turn to integrate bioreactors with sample preparation, fluidic handling, and product detection to produce micro total analytical systems (lTAS) (Easley et al 2006;Sista et al 2008;Toriello et al 2008).…”

Section: Introductionmentioning

confidence: 98%

See 2 more Smart Citations

Decreasing microfluidic evaporation loss using the HMDL method: open systems for nucleic acid amplification and analysis

Zhang

Xing

2009

Microfluid Nanofluid

View full text Add to dashboard Cite

Evaporation is of great importance when dealing with microfluidic devices with open air/liquid interfaces due to the large surface-to-volume ratio. For devices utilizing a thermal reaction (TR) reservoir to perform a series of biological and chemical reactions, excessive heatinduced microfluidic evaporation can quickly lead to reaction reservoir dry out and failure of the overall device. In this study, we present a simple, novel method to decrease heat-induced fluid evaporation within microfluidic systems, which is termed as heat-mediated diffusion-limited (HMDL) method. This method does not need complicated thermal isolation to reduce the interfacial temperature, or external pure water to be added continuously to the TR chamber to compensate for evaporation loss. The principle of the HMDL method is to make use of the evaporated reaction content to increase the vapor concentration in the diffusion channel. The experimental results have shown that the relative evaporation loss (V loss /V ini ) based on the HMDL method is not only dependent on the HMDL and TR region's temperatures (T HMDL and T TR ), but also on the HMDL and TR's channel geometries. Using the U-shaped uniform channel with a diameter of 200 lm, the V loss /V ini within 60 min is low to 5% (T HMDL = 105°C, T TR = 95°C). The HMDL method can be used to design open microfluidic systems for nucleic acid amplification and analysis such as isothermal amplification and PCR thermocycling amplification, and a PCR process has been demonstrated by amplifying a 135-bp fragment from Listeria monocytogenes genomic DNA.

show abstract

Section: Comparison Of Different Methods To Decrease/ Prevent Liquid mentioning

confidence: 99%

Section: Introductionmentioning

confidence: 98%

Section: Introductionmentioning

confidence: 98%

See 1 more Smart Citation

Decreasing microfluidic evaporation loss using the HMDL method: open systems for nucleic acid amplification and analysis

Zhang

Xing

2009

Microfluid Nanofluid

View full text Add to dashboard Cite

show abstract

“…From point of view of LOC material, two main requirements have to be fulfilled: (1) optical transparency necessary for introduction of the fluorescence excitation light and collection of the fluorescence light from an area of interest within the chip, and (2) low autofluorescence of LOC's material to minimize optical background signal. The chips can be whole made as multilayer construction of light-transparent materials -like glass, PDMS, SU-8, COC or other polymers, or only a top cover of the chip is made of these materials whereas body of the chip is made of silicon or ceramic [4][5][6][7][8][9][10][11]. It is also possible to make LOC from non-transparent materials like low-temperature co-fired ceramics (LTCC) but an integration of optical waveguides enabling introduction of the fluorescence inducing light into a detection chamber and covered with glass or PDMS is necessary [12].…”

Section: Detection Of Fluorescence In Locmentioning

confidence: 99%

“…In the detection channel, fluorescence light emitted by the fluorochrome is collected by the microscope objective with proper magnification, and guided to a photodetector by passing through filter(s) and dichroic mirror(s) to exclude the excitation light. Common detectors are cooled photomultiplier tubes (PMT) [5,6,11], semiconductor photodiodes [7,9,10], rather cooled charge coupled devices (CCD) as sensing matrix in video cameras [4] or lines in spectrophotometers [8].…”

Section: Detection Of Fluorescence In Locmentioning

confidence: 99%

Lab-on-a-chip fluorescence detection with image sensor and software-based image conditioning

Walczak¹

2011

Bulletin of the Polish Academy of Sciences: Technical Sciences

View full text Add to dashboard Cite

Abstract. The fluorometric detection in lab-on-a-chip devices for life-sciences is one of the main detection methods. Well know from "large-scale" analytical chemistry methodologies and instrumentation has been applied in lab-on-a-chip solutions. In most cases, optical instrumentation for fluorescence induction and detection is based on configuration and components borrowed from large laboratory instruments. As a result, optical instrumentation surrounding lab-on-a-chip is bulky, expensive and dedicated for operation only inside laboratory. In this paper a discussion on fluorescence detection in lab-on-a-chip is carried out. A novel image sensor -based detection instrumentation co-working with a "clever" software is described. Instrumentation is dedicated for operation in portable and low-cost devices for different life-science applications. Examples of applications of the novel method and instrumentation co-working with various lab-on-a-chips are presented on the base of author's works.

show abstract

Application of ultra‐rapid qPCR and DNA chips for viral RNA detection and confirmation

Kim

Cho

Yoon

2018

Biotech and App Biochem

View full text Add to dashboard Cite

The rapid and accurate detection of the presence of microorganisms, such as viruses, has been an important issue in the fields of public health, as well as agriculture. A PCR-based detection method has been developed and applied in these fields to determine the presence of specific pathogens. Although the major advantage of real-time PCR is the monitoring of amplification and ability to quantify the template genes, the method described here should solve the problem of nonspecific product synthesis. We obtained viral RNA from infected samples by freezing and thawing; we rapidly synthesized cDNA from RNA, and then amplified the cDNA by rapid PCR in 10 Min. Finally, the PCR products were hybridized and quickly confirmed to be the target analyte on a DNA chip. Our newly proposed methods overcome the drawbacks of PCR-based detection and provide three additional advantages, namely, rapidly obtaining large amounts of RNA from samples, quickly detecting infective or pathogenic genes, and speedily confirming the detected exogenous genes. This application might be useful for detecting viral RNA and for the diagnosis of RNA virus-mediated diseases.

show abstract

Clinical evaluation of micro-scale chip-based PCR system for rapid detection of hepatitis B virus

Cited by 73 publications

References 43 publications

Decreasing microfluidic evaporation loss using the HMDL method: open systems for nucleic acid amplification and analysis

Decreasing microfluidic evaporation loss using the HMDL method: open systems for nucleic acid amplification and analysis

Lab-on-a-chip fluorescence detection with image sensor and software-based image conditioning

Application of ultra‐rapid qPCR and DNA chips for viral RNA detection and confirmation

Contact Info

Product

Resources

About