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The possibility of performing fast and small-volume nucleic acid amplification and analysis on a single chip has attracted great interest. Devices based on this idea, referred to as micro total analysis, microfluidic analysis, or simply ‘Lab on a chip’ systems, have witnessed steady advances over the last several years. Here, we summarize recent research on chip substrates, surface treatments, PCR reaction volume and speed, architecture, approaches to eliminating cross-contamination and control and measurement of temperature and liquid flow. We also discuss product-detection methods, integration of functional components, biological samples used in PCR chips, potential applications and other practical issues related to implementation of lab-on-a-chip technologies.

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Automatic 3D extraction of building roofs from remotely sensed data is important for many applications including city modelling. This paper proposes a new method for automatic 3D roof extraction through an effective integration of LIDAR (Light Detection And Ranging) data and multispectral orthoimagery. Using the ground height from a DEM (Digital Elevation Model), the raw LIDAR points are separated into two groups. The first group contains the ground points that are exploited to constitute a 'ground mask'. The second group contains the non-ground points which are segmented using an innovative image line guided segmentation technique to extract the roof planes. The image lines are extracted from the grey-scale version of the orthoimage and then classified into several classes such as 'ground', 'tree', 'roof edge' and 'roof ridge' using the ground mask and colour and texture information from the orthoimagery. During segmentation of the non-ground LIDAR points, the lines from the latter two classes are used as baselines to locate the nearby LIDAR points of the neighbouring planes. For each plane a robust seed region is thereby defined using the nearby non-ground LIDAR points of a baseline and this region is iteratively grown to extract the complete roof plane. Finally, a newly proposed rule-based procedure is applied to remove planes constructed on trees. Experimental results show