Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

Zhang, Chunsun; Xing, Da

doi:10.1093/nar/gkm389

Cited by 380 publications

(298 citation statements)

References 94 publications

(510 reference statements)

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“…Several companies, including NorChip AS, have developed Labon-a-chip technology (Zhang and Xing, 2007). This technology has already been adapted for performing hands-free Nucleic Acid Diagnostic (NAD) (Gulliksen et al, 2005), including complete extraction of RNA and DNA (Baier et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

A comprehensive evaluation of the accuracy of cervical pre-cancer detection methods in a high-risk area in East Congo

et al. 2010

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BACKGROUND: Given the high burden of cervical cancer in low-income settings, there is a need for a convenient and affordable method for detecting and treating pre-cancerous lesions. METHODS: Samples for comparing the accuracy of cytology, virology and histology were collected. Identification of HPV E6/E7 mRNA was performed using PreTect HPV-Proofer. HPV DNA detection was performed by GP5 þ /6 þ PCR, followed by reverse line blot (RLB) for typing. RESULTS: A total of 343 women, aged 25 -60 years, attending gynaecological polyclinics in DR Congo were included for sample enrolment. The test positivity rate was conventional and liquid-based cytology (LBC) at cutoff ASCUS þ of 6.9 and 6.6%, respectively; PreTect HPV-Proofer of 7.3%; and consensus DNA PCR for 14 HR types of 18.5%. Sixteen cases of CIN2 þ lesions were identified. Of these, conventional cytology identified 66.7% with a specificity of 96.2%, LBC identified 73.3% with a specificity of 96.9%, all at cutoff ASCUS þ. HR-HPV DNA detected all CIN2 þ cases with a specificity of 85.9%, whereas PreTect HPV-Proofer gave a sensitivity of 81.3% and a specificity of 96.6%. CONCLUSION: Both HPV detection assays showed a higher sensitivity for CIN2 þ than did cytological methods. Detecting E6/E7 mRNA from only a subset of HR HPVs, as is the case with PreTect HPV-Proofer, resulted in a similar specificity to cytology and a significantly higher specificity than consensus HR HPV DNA (Po0.0001).

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Section: Discussionmentioning

confidence: 99%

A comprehensive evaluation of the accuracy of cervical pre-cancer detection methods in a high-risk area in East Congo

et al. 2010

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“…Sample pre-treatment is also important for polymerase chain reaction (PCR) amplification (Zhang and Xing, 2007;Bartlett and Stirling, 2003), since nucleic acids must be first extracted from cells, and residual material can degrade the PCR amplification efficiency.…”

Section: Sample Preparationmentioning

confidence: 99%

Towards autonomous lab-on-a-chip devices for cell phone biosensing

Comina

Suska

Filippini

2016

Biosensors and Bioelectronics

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Modern cell phones are a ubiquitous resource with a residual capacity to accommodate chemical sensing and biosensing capabilities. From the different approaches explored to capitalize on such resource, the use of autonomous disposable lab-on-a-chip (LOC) devices conceived as only accessories to complement cell phones underscores the possibility to entirely retain cell phones ubiquity for distributed biosensing. The technology and principles exploited for autonomous LOC devices are here selected and reviewed focusing on their potential to serve cell phone readout configurations. Together with this requirement, the central aspects of cell phones resources that determine their potential for analytical detection are examined. The conversion of these LOC concepts into universal architectures that are readable on unaccessorized phones is discussed within this context. (C) 2015 Elsevier B.V. All rights reserved.

Funding Agencies|Swedish Research Council (VR) [C0453801]; Carl Tryggers Foundation [CTS14140]

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“…MLoCs can automate complex assays normally performed in a laboratory onto miniaturized, portable chips with minimal reagent requirements [33], and be utilized for sample processing, purification, blood fractioning or even basic PCR [30,113,114]. Antibody, antigen, nucleic acid and cell-counting assays that require multiple reagents, capture molecules, fluid handling and detection (e.g., fluorescence, surface plasmon resonance, surface-enhanced Raman scattering, mass spectrometry, electrophoresis and electrical conductance) modalities can be supported by the devices [115][116][117][118][119][120][121][122].…”

Section: Future Perspectivementioning

confidence: 99%

HIV Diagnostics: Challenges and Opportunities

Wong

Hewlett

2010

HIV Ther.

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Accurate HIV diagnostic testing continues to pose challenges, but there are also opportunities for assay performance improvements in key areas for specific intended-use settings. The genetic diversity of HIV can result in false and discordant results in assays that fail to detect new variant strains. The use of antiretroviral therapies has resulted in drug-resistant variants that require monitoring by sequencing and genotyping methods. Nucleic acid testing is the most sensitive and reliable platform for detection, but it is costly and limited to centralized testing facilities, making implementation difficult in resource-limited settings where HIV has hit the hardest. Rapid antibody tests suitable for point-of-care use are becoming more accessible in resource-limited settings, but these tests may not detect HIV during the acute infection stage. Emerging antigen/antibody combination assays are viable alternatives to nucleic acid testing for diagnosis of recent infections. Although patient monitoring (e.g., via CD4+ T-cell count and viral load determination) and infant diagnoses still rely on clinical laboratory-based testing, point-of-care options are being developed. There are other technical challenges to HIV diagnostic testing and emerging biodetection technologies that may be able to address them, but they are not yet proven.

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Miniaturized PCR chips for nucleic acid amplification and analysis: latest advances and future trends

Cited by 380 publications

References 94 publications

A comprehensive evaluation of the accuracy of cervical pre-cancer detection methods in a high-risk area in East Congo

A comprehensive evaluation of the accuracy of cervical pre-cancer detection methods in a high-risk area in East Congo

Towards autonomous lab-on-a-chip devices for cell phone biosensing

HIV Diagnostics: Challenges and Opportunities

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