Clinical evaluation of microRNA expression profiling in non small cell lung cancer

Markou, Athina; Sourvinou, Ioanna; Vorkas, Panagiotis A.; Yousef, George M.; Lianidou, Evi

doi:10.1016/j.lungcan.2013.05.007

Cited by 175 publications

(154 citation statements)

References 41 publications

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“…Increased circulating miRNAs levels were previously reported in cancer patients, interestingly, miR-148a was described as a promising predictor biomarker to identify individuals with poor prognosis in patients with osteosarcoma, 13 miR-21 was indicative of a shorter disease free survival and overall survival in NSCLC patients 14 and miR-141 was a predictor of poor survival in advanced colon cancer. 15 In summary, this study sheds light on a group of miRNAs that are altered in TET and are emerging as putative non-invasive biomarkers for TET management.…”

Section: Discussionmentioning

confidence: 86%

Circulating miR-21-5p and miR-148a-3p as emerging non-invasive biomarkers in thymic epithelial tumors

Bellissimo

Russo

Ganci³

et al. 2015

Cancer Biology & Therapy

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Thymic epithelial cells give rise to both thymoma and thymic carcinoma. A crucial advance in thymic epithelial tumors (TET) management may derive from the identification of novel molecular biomarkers able to improve diagnosis, prognosis and treatment planning.In a previous study, we identified microRNAs that were differentially expressed in tumor vs normal thymic tissues. Among the microRNAs resulted upregulated in TET tissues, we evaluated miR-21-5p, miR-148a-3p, miR-141-3p, miR-34b-5p, miR-34c-5p, miR-455-5p as blood plasma circulating non-invasive biomarkers for TET management.We firstly report that the expression levels of specific onco-miRNAs, that we found upregulated in the blood plasma collected from TET patients at surgery, resulted significantly reduced in follow-up samples.This pilot study suggests that circulating miR-21-5p and miR-148a-3p could represent novel non-invasive biomarkers to evaluate the efficacy of therapy and the prognosis of TET.

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Section: Discussionmentioning

confidence: 86%

Circulating miR-21-5p and miR-148a-3p as emerging non-invasive biomarkers in thymic epithelial tumors

Bellissimo

Russo

Ganci³

et al. 2015

Cancer Biology & Therapy

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“…The samples were analyzed histologically to assess the level of tumor component (a minimum of 80% tumor cells) and the quality of the harvested material. Paracarcinomatous tissues adjacent to the tumor (distance from the primary tumor <2 cm) and normal tissues (distance from the primary tumor >5 cm) were defined histologically using classical pathological approaches (24).…”

Section: Methodsmentioning

confidence: 99%

Role of deregulated microRNAs in non-small cell lung cancer progression using fresh-frozen and formalin-fixed, paraffin-embedded samples

et al. 2015

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Abstract. Non-small cell lung cancer (NSCLC) is responsible for the highest number of cancer-associated mortalities worldwide, and the five-year survival rate is <15% following the initial diagnosis. MicroRNAs (miRNAs) serve important functions in a number of human diseases, including cancer. The present study investigated the expression status, clinical relevance and functional role of miRNA in NSCLC. miRNA expression profiling was performed in lung adenocarcinoma and adjacent unaffected lung tissues using 47 groups of fresh-frozen (FF) and 45 of formalin-fixed, paraffin-embedded (FFPE) samples from 11 pulmonary bulla. miR-21, -30e, -363 and -623 were further examined for differential expression in two independent cohorts. Other miRNAs, including miR-5100 and miR-650, were upregulated, while miR-10a and -26b were downregulated in FF NSCLC tissues. The associations between these miRNAs and their clinicopathological features were also investigated. miR-363, -10a and -145 were associated with lymph node status (P= 0.002, 0.005 and 0.007, respectively) and miR-650 and -145 were associated with differentiation (P=0.01 and 0.05, respectively). No associations were identified for the other miRNAs examined. In the FFPE NSCLC samples, miR-30e-5p correlated with the differentiation of the tissue (P= 0.011). The present study indicates that these miRNAs may be appropriate candidates for molecular diagnostic and prognostic markers in NSCLC.

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“…The identification of microRNAs (miRNAs) has opened a new avenue for developing highly reliable diagnostic and prognostic biomarkers to facilitate the early diagnosis of NSCLC and predict the clinical outcomes of patients (3,4). At present, numerous single miRNAs, including let-7a-2 (5), miRNA-146b (6), miRNA-155 (5,6), miRNA-31 (7), miRNA-374a (8), miRNA-451 (9) and miRNA-21 (10,11) have been reported to be associated with the tumorigenesis and progression of lung cancer.…”

Section: Introductionmentioning

confidence: 99%

Identification of microRNA-615-3p as a novel tumor suppressor in non-small cell lung cancer

Zhang

et al. 2017

Oncology Letters

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Abstract. Lung cancer is the most frequent cause of mortality in cancer patients; non-small-cell lung cancer (NSCLC) accounts for ~80% of lung cancer cases. MicroRNAs (miRNAs) have been revealed to perform an important role in cancer development and progression. Based on a custom miRNA microarray analysis of patients with NSCLC, miRNA-615-3p (miR-615-3p) downregulation was identified in NSCLC tissues compared with normal lung tissues, which suggested that miR-615-3p acted as a tumor suppressor in lung cancer. The overexpression of miR-615-3p was then validated using 40 pairs of NSCLC and adjacent normal tissue samples using a TaqMan reverse transcription-quantitative polymerase chain reaction assay. In order to investigate the tumor suppressor function of miR-615-3p, the ectopic expression of miR-615-3p in the NSCLC A549, H1299 and H1650 cell lines was established. The results revealed that overexpressed miR-615-3p markedly inhibited cell proliferation and colony formation in the 3 NSCLC cell lines compared with the cells overexpressing the negative control sequence (NC). Additional investigation revealed that miR-615-3p overexpression significantly induced apoptosis and cell cycle arrest at the G1 phase in the A549, H1299 and H1650 cell lines compared with the cells overexpressing NC. Finally, ectopic expression of miR-615-3p was found to repress the cell migration and invasion of the 3 lung cancer cell lines. The results of the present study demonstrate, for the first time, that miR-615-3p functions as a tumor suppressor in NSCLC, and may be a novel potential molecular therapeutic target for patients with NSCLC.

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Clinical evaluation of microRNA expression profiling in non small cell lung cancer

Cited by 175 publications

References 41 publications

Circulating miR-21-5p and miR-148a-3p as emerging non-invasive biomarkers in thymic epithelial tumors

Circulating miR-21-5p and miR-148a-3p as emerging non-invasive biomarkers in thymic epithelial tumors

Role of deregulated microRNAs in non-small cell lung cancer progression using fresh-frozen and formalin-fixed, paraffin-embedded samples

Identification of microRNA-615-3p as a novel tumor suppressor in non-small cell lung cancer

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