Clinical Investigations on the Corneal Endothelium

Mishima, S

doi:10.1016/s0161-6420(82)34755-7

Cited by 130 publications

(96 citation statements)

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“…In transplanted corneas, endothelium can keep its function in some cases even for 30 years [14][15][16][17], with an initial cell density above 2,500 cells/mm 2 ( Table 2). Minimal cell density at the time of PKP is reported to range between 2,000 and 2,500 cells/mm 2 and the minimal, critical cell density limit for corneal decompensation is 250-500 cells/mm 2 [14,15,[18][19][20][21]. Donor corneas with initial cell densities under 2,000 cells/mm 2 could reach this critical cell density in less than 20 years [8].…”

Section: Resultsmentioning

confidence: 99%

Assessment of endothelial cell density and corneal thickness in corneal grafts an average of 5 years after penetrating keratoplasty

Kettesy

Németh

Kemény-Beke

et al. 2014

Wien Klin Wochenschr

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SummaryBackground Corneal transparency is a useful indicator for corneal function. Our aim was to investigate central corneal endothelial cells and corneal thickness in transplanted corneas at an average of 5.4 years after penetrating keratoplastyPatients and methods The study involved 68 perforated keratoplasty patients with at least a 1 year followup. Post-operatively, the central corneal endothelial layer was observed using a contact specular microscope. Central endothelial cell density, corneal thickness and the coefficient of variation of endothelial size were statistically analysed.Results The post-operative follow-up time was ranging from 12 months to 23 years. Endothelial cell density (ECD) was 1,501 ± 249 cell/mm 2 . The average cell size was 673.6 ± 98.3 μm 2 , and the coefficient of variation of cell size was 0.61 ± 0.11. No difference in ECD was detected between diagnostic groups. Corneal thickness was 0.56 ± 0.06 mm. Correlation between ECD and post-operative time was not significant (r = 0.02; p = 0.85).Conclusion Our study concluded that ECD showed a higher rate of decrease after penetrating keratoplasty with no relation to pre-operative diagnosis.Keywords Endothelial cell loss · graft survival · penetrating keratoplasty · specular microscope Bestimmung der Endothelzelldichte und der Hornhautdicke in Hornhauttransplantaten circa 5 Jahre nach einer penetrierenden Keratoplastik Zusammenfassung Hintergrund Die Transparenz der Cornea ist ein nütz-licher Indikator der Funktion der Hornhaut. Das Ziel unserer Studie war es, die zentralen Endothelzellen der Cornea sowie die Dicke der Hornhaut in transplantierten Hornhäuten circa 5,4 (im Durchschnitt) Jahre nach penetrierender Keratoplastik zu untersuchen.Patienten und Methoden In die Studie wurden 68 Patienten nach perforierter Keratoplastik aufgenommen, die zumindest 1 Jahr nachuntersucht worden waren. Postoperativ wurde die zentrale Endothelschicht der Hornhautmittel seines Kontakt-Spekular Mikroskops untersucht. Die Dichte der zentralen Endothelzellen, die Dicke der Hornhaut und der Variations-Koeffizient der Größe des Endothels wurden statistisch analysiert.Ergebnisse Der Zeitpunkt der postoperativen Kontrollen variierte von 12 Monaten bis 23 Jahren. Die Endothelzelldichte (EZD) lag bei 1501 ± 249 Zelle/mm 2 . Der Mittelwert der Zellgröße war 673,6 ± 98,3 μm 2 und der Variationskoeffizient 0,61 ± 0,11. Es gab keinen Unterschied in der EZD zwischen den nach Diagnosen eingeteilten Gruppen. Die Korrelation zwischen der EZD und dem Zeitabstand zur Operation war nicht kant (r = 0,02; p = 0,85).Schlussfolgerung Unsere Studie zeigt unabhängig von der präoperativen Diagnose eine abfallende Tendenz der EZD nach penetrierender Keratoplastik.

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Section: Resultsmentioning

confidence: 99%

Assessment of endothelial cell density and corneal thickness in corneal grafts an average of 5 years after penetrating keratoplasty

Kettesy

Németh

Kemény-Beke

et al. 2014

Wien Klin Wochenschr

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“…Microscopy of the corneal endothelium can be used to evaluate the functional reserve of the cornea and to provide better understanding the wound healing process following intraocular surgery (Mishima 1981, Tuft & Coster 1990. Previous reports have demonstrated that the use of specular microscope to determine endothelial cell density is a practical way to evaluate corneal endothelium for eyes that have been harvested for tissue banks or corneal transplantation (Andrew et al 2001).…”

Section: Discussionmentioning

confidence: 99%

Morphological analysis of the corneal endothelium in eyes of dogs using specular microscopy

et al. 2008

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Pesq. Vet. Bras. 28(9):427-430, setembro 2008 RESUMO.-[Análise morfológica com microscopia especular do endotélio corneal em olhos de cães.] Foram estudados 20 olhos de 10 cães sem raça definida, machos e fêmeas com 6 anos de idade. A morfologia das células do endotélio da córnea foi analisada utilizando-se microscópio especular de contato. Foram estudadas as regiões central e periférica da córnea. De cada região da córnea foram realizadas três micrografias. De cada micrografia foram analisadas no mínimo 100 células endoteliais. Foram obtidos os valores do polimegatismo e pleomorfismo. O endotélio corneano de cães caracterizou-se por uma monocamada de células poligonais uniformes em tamanho e forma. A forma predominante das células endoteliais foi hexagonal. O índice de polimegatismo foi 0.22. Este estudo demonstrou que a morfologia das células do endotélio da córnea de cães é semelhante à observada em humanos. INTRODUCTIONThe corneal endothelium is a single layer of polygonal cells on the inner surface of the cornea. The endothelial integrity and metabolic activity are essential for continuous maintenance of corneal dehydration and transparency (Waring et al. 1982). Ageing results in a gradual reduction in endothelial cell density and an increase in cell area variability in those species that exhibit minimal mitotic potential (Jackson et al. 1995). Furthermore, morphological changes of endothelium associated with cell loss occur in many conditions including corneal dystrophies, keratoconus, glaucoma, uveitis, blunt ocular trauma, cataract ex-

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“…15 The wounded endothelium repairs itself primarily through limited migration and hypertrophy, and secondarily through cell division. 16 Mass culture of the human endothelial cells done in vitro showed that endothelial cells in persons younger than 20 yrs grow better than older ones. 17 Although cells adjacent to the wound participate most actively in the early reestablishment of an intact endothelial layer, the entire endothelial population may participate to some degree.…”

Section: Discussionmentioning

confidence: 99%