Clinical, Molecular and Histopathological Evaluation of the Effect Autologus PRP & PRF on the Second Intension Wound Healing in Dogs

Abstract

A new trend of regenerative medicine was introduced recently in the field of second intension wound healing. The PRP and PRF represented a corner stone in this clinical practice, were it admit many factors for acceleration of wound healing, enhancement of wound epithelization and neovascularization. PRP and PRF are considered as a great concentrated source of growth factors (PDGF, TGF-β, VEGF, bFGF) and cytokines which are fundamental fro wound healing. Nine adult dogs were used in this study; we induced a 3cm diameter full thickness cutaneous injury at the right chest region. The animals were divided into three groups equally and the wounds were treated twice weekly for three successive weeks. Group A received only normal saline (control group). The members of second group treated by PRP (group B) while, the group C treated by PRF. Clinical evaluation, Molecular studies of IL10 & TGF-β and histopathological examination were used to demonstrate the difference between the three treatment regimes. Results showed non-significant negative low correlation between loss of weight and WHR%, and showed significant high positive correlation between treatment cost either by PRP or PRF with IL10 (0.79*) and WHR%(0.996**). We concluded that the PRP and PRF exhibited higher regeneration capacity and accelerate the quality of wound healing. The PRP was more superior to PRF, but not significantly different. The IL10 was significant increase in expression in PRP, while TGF-b was non-significant increased in PRF group