Clinical outcome of cryopreserved human pronuclear stage embryos resulting from intracytoplasmic sperm injection

Comparison of cryopreservation outcome following intracytoplasmic sperm injection and conventional in vitro fertilization

“…The abortion rate of clinical pregnancies was 22% for frozen-thawed conventional IVF embryos and 26.8% for ICSl-derived embryos. These rates were comparable and corroborate previous studies demonstrating that the abortion rates for frozen-thawed ET cycles ranged between 20 and 23% for conventional IVF embryos and 18 and 25% for lCSl-derived embryos (8,9,13).…”

“…A1-Hasani et al (8) reported a survival rate of 78 and 76.5% for zygotes derived from conventional IVF and ICSI, respectively. A higher survival rate for freezing of pronuclear-stage zygotes, 94.8 and 93.2% for IVFand ICSI-derived zygotes, was recently reported by Hoover et al (9). These reported rates as well as our results suggest that cryopreservation is not detrimental to ICSI-derived embryos of any stage, yielding a survival rate comparable to that of embryos originating from conventional IVE A previous report (7) showed that the preclinical wastage of pregnancies is significantly higher for cryopreserved ICSI embryos compared to conventional IVF: 40.9 and 27%, respectively.…”

“…For fresh ET the pregnancy rate for ICSI cycles seems to be comparable to that of conventional IVF attempts (5,6). This rate is somewhat lower for frozen-thawed ET cycles of both ICSI and conventional IVF and is reported to range between 12.9 and 21% (7)(8)(9)(10)(11)(12)(13).…”

Artificial shrinkage of blastocoel using a laser pulse prior to vitrification improves clinical outcome

“…Cryopreservation at different stages of embryonic development has contributed to variability in clinical outcome [12][13][14][15]. Zygotes [16][17][18][19], cleavage stage embryos [20,21], and blastocysts [22][23][24] have been successfully vitrified, but superior pregnancy rate has been observed with blastocysts, possibly due to their size or multicellular structure [25]. However, expanded blastocysts with a large amount of fluid in the blastocoel have been shown to have lower survival rates than less mature blastocysts or morula-stage embryos [26].…”

Use of Silicone Elastomer-Based Microfluidic Devices and Systems in Reproductive Technologies

“…The fertilization rate can be assessed indirectly by examination of the cleavage rate 40-42 h post insemination. The basic procedures of IVF in cattle and humans are similar [33]. Another in vitro procedure is intracytoplasmic sperm injection (ICSI), where a selected single sperm is directly injected into the oocyte.…”