Clinical outcome of dogs with grade-II mast cell tumors treated with surgery alone: 55 cases (1996–1999)

Séguin, Bernard; Leibman, Nicole F.; Bregazzi, Victoria S.; Ogilvie, Gregory K.; Powers, Barbara E.; Dernell, William S.; Fettman, Martin J.; Withrow, Stephen J.

doi:10.2460/javma.2001.218.1120

Cited by 130 publications

(198 citation statements)

References 12 publications

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“…Some reports indicated that moderate differentiated mast cell tumors can have a favorable prognosis (if treated appropriately) as in the case of highly differentiated mast cell tumors (Seguin et al, 2001;Weisse et al, 2002;Murphy, 2003). The survival rate is around 3.5 years in about 55% of subjects (Ginn et al, 2007).…”

Section: Resultsmentioning

confidence: 99%

Epidemiological Aspects and Differential Diagnosis of the Cutaneous Round Cell Tumors in Dogs

Cora¹,

Gal²,

Taulescu³

et al. 2017

BUASVMCN-VM

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Round cell neoplasms (RCNs) are frequent cutaneous lesions in dogs, with high percentages among skin tumors. In this category are included histiocytoma, mast cell tumor, plasmacytoma, lymphoma and transmissible venereal tumor. The aim of the study was to perform an epidemiological study with reference to the cutaneous round cell tumors in a period of 10 years in the Department of Pathology (Faculty of Veterinary Medicine, ClujNapoca, Romania). Additionally, in the recorded cases with round cell tumors (mast cell tumor, histiocytoma and lymphoma) we described the main histological and cytological features. The epidemiological data were collected from the records of Pathology Department between 2005-2014. The investigation included dogs diagnosed with cutaneous round cell neoplasms, following necropsy analysis or assessment of biopsies or cytological samples. All collected specimens were analyzed by histopathological and/or cytological techniques. The staining used for histological investigation were Hematoxylin-eosin, Masson's trichrome and Toluidine blue, whereas Diff Quikand Wright methods were utilized in cytological specimens. The distribution of the cutaneous round cell tumors in relation to age, breed and sex was also assessed. The most frequent round cell tumor type was the mast cell tumor (19.54%) followed by histiocytoma (11.33%) and lymphoma (1.98%). The round cell tumors recorded were equally distributed in both males and females. Concerning the distribution of cutaneous RCNs by age (average age), histiocytoma occurred in 5 years old subjects, mast cell tumor in 11.9 years old subjects, and lymphoma in 6 years old subjects. Mast cell tumor was more frequent in stray dogs and Boxer breed, while histiocytoma occurred more commonly in stray dogs. Histological and cytological analysis was mandatory to perform the differential diagnosis between RCNs. Microscopic details concerning cytoplasm and nucleus of tumoral cells, together with the displaying mode in histological specimens may reveal the type of RCN. Cutaneous round cell tumors had an increased incidence, aspect observed in other reports too. The differential diagnosis has to rely on some specific microscopical features that can suggest the type of RCN.

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Section: Resultsmentioning

confidence: 99%

Epidemiological Aspects and Differential Diagnosis of the Cutaneous Round Cell Tumors in Dogs

Cora¹,

Gal²,

Taulescu³

et al. 2017

BUASVMCN-VM

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“…Os dados do presente estudo corroboram a literatura , Webster et al 2007), pois os tumores nessas localizações estão relaQuadro 4. Distribuição das duas classificações histológicas, marcadores imuno-histoquímicos e análise da PCR Forrest (1997), que relatou que os mastocitomas nos membros tinham comportamento benigno, e com Oliveira (2008), que classificou a maioria dos mastocitomas no membro como de grau I. Os tumores disseminados apresentavam graus baixos e padrão perimembranoso, o que corrobora com os autores (Thamm et al 1999, Séguin et al 2001, Thamm & Vail 2001 que não os associam a piores prognósticos.…”

Section: Discussionunclassified

Comparação de duas classificações histopatológicas com o padrão de imuno-marcação para KIT, a avaliação da proliferação celular e com a presença de mutações no c-KIT de mastocitomas cutâneos caninos

Carvalho

Nardi

et al. 2017

Pesq. Vet. Bras.

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RESUMO: A graduação histopatológica é o método de eleição para prever o comportamento biológico do mastocitoma e, atualmente, são utilizados os métodos de Patnaik e de Kiupel para dividi-los em graus de malignidade. O objetivo do presente trabalho foi comparar as duas classificações histológicas com as variáveis clínicas, os marcadores imuno-histoquímicos e com a presença de mutações para verificar as características que estão mais relacionadas entre si e com os piores prognósticos. Foram avaliados 61 animais, levando em consideração o sexo, a raça, a idade, a localização tumoral, o grau tumoral pelas classificações de Patnaik e Kiupel, a infiltração de eosinófilos, a marcação do KIT e do Ki-67 e a presença de mutação. As variáveis foram correlacionadas utilizando os testes de qui-quadrado, teste de Fisher, teste de verossimilhança e o teste de risco relativo. Os animais idosos foram os mais acometidos, enquanto os animais sem raça definida e os das raças Boxer, Labrador e Pinscher foram aqueles com maior predisposição ao desenvolvimento tumoral. A localização e a idade estão associadas ao grau tumoral. Os tumores em cabeça, pescoço e região genital têm 10 vezes mais chance de serem classificados como de alto grau (RR=10,667; IC95% 1,909-59,615, p=0,004) e os idosos oito vezes mais chance (RR=8,00; IC95% 0,955-67,009; p=0,029). Os tumores de grau II e os de baixo grau foram os mais encontrados e as duas classificações histológicas demonstram correlação muito significativa entre si (p<0,001). A concentração do infiltrado eosinofílico não demonstrou correlação significativa com nenhuma das classificações histológicas. O padrão KIT foi dependente da localização tumoral (p=0,015), já que os tumores genitais, na cabeça e no pescoço possuíam 18 vezes mais chance de apresentarem padrão citoplasmático (RR=18,571; IC95% 1,954-176,490; p=0,003), e das classificações de Patnaik (p=0,001) e Kiupel (p<0,001), sendo que os tumores de alto grau têm 36 vezes mais chance de apresentarem padrão citoplasmático (RR=36,00, IC95% 4,35-297,948; p<0,001). A marcação do Ki-67 demonstrou dependência da localização (p=0,024). A presença de mutação no exon 11 do domínio justamembrana não demonstrou associação com nenhuma das variáveis clínicas, das classificações histológicas, da concentração de eosinófilos e do padrão KIT. A presença da mutação foi correlacionada significativamente apenas ao Ki-67 (p=0,010). Os resultados obtidos sugerem que a localização é a variável clínica mais relacionada ao prognóstico e que apenas a classificação de Kiupel associada à imuno-histoquímica são eficientes para avaliar o comportamento tumoral.

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“…Tumor recurrence developed in a small number of dogs with completely excised, grade II, cutaneous mast cell tumors [21,27], suggesting that a complete excision can reduce recurrence. In a prior study, the frequency of recurrence in complete surgical excision within 24 months postoperatively was significantly lower than in incomplete surgical excision [13].…”

Section: Discussionmentioning

confidence: 99%

“…In grade II tumors, a complete surgical margin accurately predicted a good prognosis [21,27]. The frequency of recurrence in the complete surgical excision cases was significantly lower than in incomplete surgical excision during 24 months following surgery [13].…”

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confidence: 92%

“…The clinical course of each dog with CCMT varied from benign to highly malignant. Various prognostic factors have been studied, including clinical stage, surgical resection [13,21,27], kinetic parameters (Ki-67 [1,19], BrDU [19], PCNA [23], AgNOR [11]), DNA ploidy [2], intratumoral vessel density [18], p53 [7,8], KIT [9,25,26], nuclear morphometry [24], tumor depth [10] and location [4].…”

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confidence: 99%

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Prognostic Significance of Surgical Margin, Ki-67 and Cyclin D1 Protein Expression in Grade II Canine Cutaneous Mast Cell Tumor

et al. 2007

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ABSTRACT. The prognosis for canine cutaneous mast cell tumor (CCMT) is thought to be correlated with histopathological grading. However, the wide variety of histopathologic types of grade II is one of the most troublesome and difficult points for prognosis. The objective of this study is to determine the prognostic value of surgical margin, ki-67 and cyclin D1 protein expression in grade II tumor. Surgically resected specimens of solitary grade II CCMT from 48 dogs with follow-up periods over 360 days (median was 1080 days) were used in this study. The expression of cyclin D1 and ki-67 proteins was determined by morphometrically using slides stained immunocytochemically, and the correlations among the results, survival rate, and recurrence and/or metastasis rate of each dog were analyzed statistically. The recurrence and/or metastasis and mortality rate in the incomplete surgical excision group within 30 months postoperatively were higher than that of the complete surgical excision group. In the incomplete surgical excision group, dogs with low positive staining of ki-67 had a significantly better survival, but the recurrence and metastasis rate and ki-67 positivity failed to show a significant correlation. Only a small number of cases showed cyclin D1-positive tumor cells, but most of them had a poor outcome with a high recurrence rate. In grade II CCMT, incomplete excision induced a relatively high metastasis rate and poor prognosis. Ki-67 positivity is a marker for the estimation of overall survival in incomplete surgical excision cases. Cyclin D1 positivity was low and may not have a prognostic role. KEY WORDS: canine, cyclin D1, ki-67, mast cell tumor, skin.J. Vet. Med. Sci. 69(11): 1117-1121, 2007 Cutaneous mast cell tumors (CCMT) are the most common round cell neoplasm in dogs, accounting for 7 to 21% of all skin tumors [12,16] The most accurate and commonly used prognostic factor is histological grade. Among the histological grading systems, Patnaik's method has been the most commonly used around the world [3,17], and the prognostic significance of this method was confirmed in other studies [1,7,8,23]. According to Patnaik's system, CCMT is classified into 3 categories on the basis of morphologic grading: grade I tumors are the most differentiated and have a good prognosis, grade III tumors are poorly differentiated with a poor prognosis, and grade II shows an intermediate differentiation with good to poor prognosis. Using this grading system, the prognosis of grade I and III tumors can be readily determined well [17]. However, other parameters have been needed for precise prognosis of grade II tumors, which have shown an inconsistent prognosis, the marked degree of inter-observer variation and accounted for 40% or 59% of CCMT [15,17].The relationships among surgical margin and prognosis or recurrence have been reported by several researchers. In grade II tumors, a complete surgical margin accurately predicted a good prognosis [21,27]. The frequency of recurrence in the complete surgical excision cases w...

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Clinical outcome of dogs with grade-II mast cell tumors treated with surgery alone: 55 cases (1996–1999)

Abstract: Results suggest that additional local treatment may not be required after complete excision of grade-II mast cell tumors and that most dogs do not require systemic treatment.

Cited by 130 publications

References 12 publications

Epidemiological Aspects and Differential Diagnosis of the Cutaneous Round Cell Tumors in Dogs

Epidemiological Aspects and Differential Diagnosis of the Cutaneous Round Cell Tumors in Dogs

Comparação de duas classificações histopatológicas com o padrão de imuno-marcação para KIT, a avaliação da proliferação celular e com a presença de mutações no c-KIT de mastocitomas cutâneos caninos

Prognostic Significance of Surgical Margin, Ki-67 and Cyclin D1 Protein Expression in Grade II Canine Cutaneous Mast Cell Tumor

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