Clinical Potential of microRNA-7 in Cancer

Horsham, Jessica L.; Kalinowski, Felicity C.; Epis, Michael R.; Ganda, Clarissa; Brown, Rikki A. M.; Leedman, Peter J.

doi:10.3390/jcm4091668

Cited by 37 publications

(33 citation statements)

References 98 publications

(159 reference statements)

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“…Extensive roles of miR-7 in regulating cancer cell initiation, proliferation, migration, invasion, survival and death by targeting a number of oncogenic signaling pathways have been reported [27, 28]. Partially consist with the role of miR-7 in down-regulating FAK and inhibiting the ERK/MAPK signaling pathway, miR-7 overexpression is associated with suppressed proliferation, cell migration and tumorigenicity, and induced cell apoptosis in NSCLC cells [29].…”

Section: Discussionmentioning

confidence: 99%

MicroRNA-7 inhibits cell proliferation, migration and invasion in human non-small cell lung cancer cells by targeting FAK through ERK/MAPK signaling pathway

et al. 2016

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ObjectiveTo investigate the effects of microRNA-7 (miR-7) on the proliferation, migration and invasion of non-small cell lung cancer NSCLC) cells by targeting FAK through ERK/MAPK signaling pathway.MethodsNSCLC tissues and adjacent normal tissues were obtained from 160 NSCLC patients after operation. NSCLC cell lines (A549, H1299 and H1355) and a normal human fetal lung fibroblast cell line (MRC-5) were obtained. NSCLC cells were assigned into miR-7 inhibitors, miR-7 mimics, blank, miR-7 mimics control, miR-7 inhibitors control, FAK siRNA and miR-7 inhibitors + FAK siRNA groups. The expressions of miR-7 and FAK mRNA in tissues and cell lines were detected by qRT-PCR and Western-Blotting. Cell proliferation, migration and invasion were detected by MTT assay, wound scratch assay and Transwell assay.ResultsCompared with adjacent normal tissues, miR-7 expression was down-regulated, but the mRNA and protein expressions of FAK, ERK and MAPK were up-regulated. Compared with the blank and mimics control groups, miR-7 significantly increased but FAK, ERK and MAPK expressions decreased in miR-7 mimics and FAK siRNA groups. Cell proliferation, migration and invasion were inhibited in the miR-7 mimics and FAK siRNA groups, while opposite regarding miR-7 inhibitors group.ConclusionThe miR-7 can inhibit the activation of ERK/MAPK signaling pathway by down-regulating FAK expression, thereby suppressing the proliferation, migration and invasion of NSCLC cells. The miR-7 and its target gene FAK may be novel targets for the diagnosis and treatment of NSCLC.

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Section: Discussionmentioning

confidence: 99%

MicroRNA-7 inhibits cell proliferation, migration and invasion in human non-small cell lung cancer cells by targeting FAK through ERK/MAPK signaling pathway

et al. 2016

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“…Although miRNAs play key roles during normal developmental processes, deregulation of miRNA expression has been noted in several human cancers in which miRNAs have been shown to have both oncogenic and tumor suppressor functions . miR‐7‐5p was downregulated and functions as a potent tumor suppressor in multiple cancer types, including breast cancer . In prior research, miR‐7 expression was negatively correlated with stage, grade and survival for breast cancer patients, and overexpression of miR‐7 inhibited the proliferation, invasion, and migration of breast cancer cells …”

Section: Introductionmentioning

confidence: 98%

hsa_circRNA_0006528 as a competing endogenous RNA promotes human breast cancer progression by sponging miR‐7‐5p and activating the MAPK/ERK signaling pathway

Gao

Zhang

et al. 2018

Molecular Carcinogenesis

114

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Emerging research has indicated that circular RNAs (circRNAs), a novel class of noncoding RNAs, play a vital role in human tumorigenesis and progression. Our previous results suggested that hsa_circ_0006528 (circ_0006528), a circRNA with an unknown function, mediates adriamycin resistance in human breast cancer cells. However, the role of circ_0006528 in breast cancer progression remains unknown. Here, we investigated the probable involvement of circ_0006528 in breast cancer. We analyzed a cohort of 97 patients and found that circ_0006528 expression was significantly upregulated in human breast cancer tissues compared with that in adjacent nontumorous tissues and was significantly associated with advanced tumor-nodemetastasis (TNM) stage and poor prognosis. In addition, we found that in breast cancer cells, circ_0006528 could promote DNA synthesis and cell proliferation, invasion, and migration. Downregulating circ_0006528 induced G2 phase arrest and cell apoptosis. Further mechanistic studies revealed that circ_0006528 could sponge endogenous miR-7-5p and inhibit its activity. We also identified Raf1, which activates the MAPK/ERK signaling pathway, as a target of miR-7-5p and determined that circ_0006528 promotes breast cancer growth, invasion, and migration by promoting the expression of Raf1 and activates the MAPK/ERK pathway. Thus, this study provides the first evidence of the circ_0006528/miR-7-5p/Raf1/MEK/ERK regulatory network in the development of breast cancer and suggests that circ_0006528 is a potential therapeutic target and prognostic predictor for breast cancer.breast cancer, hsa_circ_0006528, invasion, migration, miR-7-5p, proliferation | INTRODUCTIONBreast cancer is the most prevalent cancer in women worldwide and represents a serious public health problem. One complication in the clinical management of breast cancer is the tendency of malignant cells to shed into the circulation during the early stages of tumor development, 1,2 causing the formation of metastatic foci, a phenomenon that directly contributes to approximately 90% of breast cancerrelated mortality. 3,4 The precise genetic and epigenetic mechanisms that regulate the formation and malignant progression of tumors remain unclear and require further study. Therefore, it is essential to Danfeng Gao, Xiaowei Qi, and Xiufen Zhang contributed equally to this work. |

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“…The miRNAs are involved in various cellular processes of cancer development, including cell proliferation, differentiation, migration and invasion [12–15]. Emerging evidence has identified that the deregulation of miRNAs is related to initiation of various cancers, such as bladder cancer, gastric cancer, lung cancer, and breast cancer [16, 17].…”

Section: Introductionmentioning

confidence: 99%

miR-204 suppresses the development and progression of human glioblastoma by targeting ATF2

Song

Fajol

et al. 2016

Oncotarget

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In human cancers, miRNAs are important regulators of multiple cellular processes, and aberrant miRNA expression has been observed, and their alterations contribute to multiple cancer development and progression. Till now, little has been known about the role of miR-204 in human glioblastoma (GBM). In the present study, we used in-vitro assays to investigate the mechanisms of miR-204 in GBM cell lines and 60 cases of GBM tissues. Here, we found that miR-204 expression is downregulated in both GBM cell lines A172, U87 and U251 cells and GBM tissues as compared with NHA cells and normal tissues (all p<0.001). In addition, the ectopic expression of miR-204 suppressed A172 and U87 cell proliferation, migration and invasion. Meanwhile, miR-204 over-expression extremely inhibited the protein expression of ATF2. Notably, the enforced expression of ATF2 in A172 and U87 cells with the over-expression of miR-204 attenuated the inhibitory effects of miR-204 on proliferation, migration and invasion. In conclusion, our findings suggest that miR-204 suppressed cell proliferation, migration and invasion through inhibition of ATF2, thus, miR-204 may function as a useful drug target in the treatment and diagnosis of GBM.

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Clinical Potential of microRNA-7 in Cancer

Cited by 37 publications

References 98 publications

MicroRNA-7 inhibits cell proliferation, migration and invasion in human non-small cell lung cancer cells by targeting FAK through ERK/MAPK signaling pathway

MicroRNA-7 inhibits cell proliferation, migration and invasion in human non-small cell lung cancer cells by targeting FAK through ERK/MAPK signaling pathway

hsa_circRNA_0006528 as a competing endogenous RNA promotes human breast cancer progression by sponging miR‐7‐5p and activating the MAPK/ERK signaling pathway

miR-204 suppresses the development and progression of human glioblastoma by targeting ATF2

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