Clinical relevance and virulence factors of pigmented Serratia marcescens

Carbonell, Gleize Villela

doi:10.1016/s0928-8244(00)00146-2

Cited by 18 publications

(28 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…marcescens (99% identity) and forms part of a monophyletic S. marcescens cluster with a high level of statistical support. As a result, S. liquefaciens MG1 was reclassified as S. marcescens MG1 and is thus a nonpigmented S. marcescens strain, which is typical of pathogenic strains (10,25).…”

Section: Resultsmentioning

confidence: 99%

Quorum-Sensing Regulation of Adhesion in Serratia marcescens MG1 Is Surface Dependent

et al. 2007

View full text Add to dashboard Cite

Serratia marcescens is an opportunistic pathogen and a major cause of ocular infections. In previous studies of S. marcescens MG1, we showed that biofilm maturation and sloughing were regulated by N-acyl homoserine lactone (AHL)-based quorum sensing (QS). Because of the importance of adhesion in initiating biofilm formation and infection, the primary goal of this study was to determine whether QS is important in adhesion to both abiotic and biotic surfaces, as assessed by determining the degree of attachment to hydrophilic tissue culture plates and human corneal epithelial (HCE) cells. Our results demonstrate that while adhesion to the abiotic surface was AHL regulated, adhesion to the HCE cell biotic surface was not. Type I fimbriae were identified as the critical adhesin for non-QS-mediated attachment to the biotic HCE cell surface but played no role in adhesion to the abiotic surface. While we were not able to identify a single QS-regulated adhesin essential for attachment to the abiotic surface, four AHL-regulated genes involved in adhesion to the abiotic surface were identified. Interestingly, two of these genes, bsmA and bsmB, were also shown to be involved in adhesion to the biotic surface in a non-QS-controlled fashion. Therefore, the expression of these two genes appears to be cocontrolled by regulators other than the QS system for mediation of attachment to HCE cells. We also found that QS in S. marcescens regulates other potential cell surface adhesins, including exopolysaccharide and the outer membrane protein OmpX. We concluded that S. marcescens MG1 utilizes different regulatory systems and adhesins in attachment to biotic and abiotic surfaces and that QS is a main regulatory pathway in adhesion to an abiotic surface but not in adhesion to a biotic surface.

show abstract

Section: Resultsmentioning

confidence: 99%

Quorum-Sensing Regulation of Adhesion in Serratia marcescens MG1 Is Surface Dependent

et al. 2007

View full text Add to dashboard Cite

show abstract

“…Of these strains, three are clinical isolates and two are environmental. It has long been observed that nonpigmented antibiotic-resistant strains of S. marcescens are much more common in the clinical setting than in the natural environment (Aucken & Pitt, 1998;Carbonell et al, 2000;Grimont & Grimont, 1978). There is no obvious reason for the prevalence of non-pigmented clinical strains, Fig.…”

Section: Differences In Pig Cluster Regulationmentioning

confidence: 99%

The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation

et al. 2004

View full text Add to dashboard Cite

The prodigiosin biosynthesis gene cluster (pig cluster) from two strains of Serratia (S. marcescens ATCC 274 and Serratia sp. ATCC 39006) has been cloned, sequenced and expressed in heterologous hosts. Sequence analysis of the respective pig clusters revealed 14 ORFs in S. marcescens ATCC 274 and 15 ORFs in Serratia sp. ATCC 39006. In each Serratia species, predicted gene products showed similarity to polyketide synthases (PKSs), non-ribosomal peptide synthases (NRPSs) and the Red proteins of Streptomyces coelicolor A3(2). Comparisons between the two Serratia pig clusters and the red cluster from Str. coelicolor A3(2) revealed some important differences. A modified scheme for the biosynthesis of prodigiosin, based on the pathway recently suggested for the synthesis of undecylprodigiosin, is proposed. The distribution of the pig cluster within several Serratia sp. isolates is demonstrated and the presence of cryptic clusters in some strains shown. The pig cluster of Serratia marcescens ATCC 274 is flanked by cueR and copA homologues and this configuration is demonstrated in several S. marcescens strains, whilst these genes are contiguous in strains lacking the pig cluster.

show abstract

“…S. marcescens is ubiquitous in nature and can be found in water, soil, plants, insects, and animals (1). While tetracycline resistance is reportedly widespread in S. marcescens (6,12,15,38), the only characterized S. marcescens tetracycline resistance determinant is the Tet(B) protein expressed by the conjugative plasmid R478 (13).…”

mentioning

confidence: 99%

Novel Tetracycline Resistance Determinant Isolated from an Environmental Strain of Serratia marcescens

Thompson

Maani

Lindell

et al. 2007

Appl Environ Microbiol

View full text Add to dashboard Cite

Resistances to tetracycline and mercury were identified in an environmental strain of Serratia marcescens isolated from a stream highly contaminated with heavy metals. As a step toward addressing the mechanisms of coselection of heavy metal and antibiotic resistances, the tetracycline resistance determinant was cloned in Escherichia coli. Within the cloned 13-kb segment, the tetracycline resistance locus was localized by deletion analysis and transposon mutagenesis. DNA sequence analysis of an 8.0-kb region revealed a novel gene [tetA (41) (41) promoters were analyzed using lacZ fusions, which showed that the expression of both the tetA(41) and tetR(41) genes exhibited TetR(41)-dependent regulation by subinhibitory concentrations of tetracycline. The apparent lack of plasmids in this S. marcescens strain, as well as the presence of metabolic genes adjacent to the tetracycline resistance locus, suggested that the genes were located on the S. marcescens chromosome and may have been acquired by transduction. The cloned Tet 41 determinant did not confer mercury resistance to E. coli, confirming that Tet 41 is a tetracycline-specific efflux pump rather than a multidrug transporter.

show abstract

Clinical relevance and virulence factors of pigmented Serratia marcescens

Cited by 18 publications

References 16 publications

Quorum-Sensing Regulation of Adhesion in Serratia marcescens MG1 Is Surface Dependent

Quorum-Sensing Regulation of Adhesion in Serratia marcescens MG1 Is Surface Dependent

The Serratia gene cluster encoding biosynthesis of the red antibiotic, prodigiosin, shows species- and strain-dependent genome context variation

Novel Tetracycline Resistance Determinant Isolated from an Environmental Strain of Serratia marcescens

Contact Info

Product

Resources

About