Clinical Relevance of Alkaline Phosphatase Isoenzyme Determinations by High Performance Liquid Chromatography

Schönaü, Eckhard; Herzog, K. H.; Böhles, H

doi:10.1515/cclm.1986.24.9.641

Cited by 2 publications

(2 citation statements)

References 10 publications

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“…The HPLC elution behaviour and the electrophoretic mobility are typical for H-Mr AP (7,19). The minor anodal electrophoretic mobility is caused by a slight siewing effect of this agarose gel.…”

Section: Discussionmentioning

confidence: 96%

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High-Molecular-Mass or Macromolecular Alkaline Phosphatase in Sera of Children with Solid Tumors

Schönaü

Beck

et al. 1994

Klin Padiatr

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High-molecular-mass alkaline phosphatase (H-Mr AP) was detected in sera from children with solid tumors without liver metastases. H-Mr AP activities were determined by a liquid chromatographic and an electrophoretic method. In 5 out of 10 cases with solid tumors--Ewing sarcoma (n = 2), neuroblastoma (n = 2), and rhabdoid tumor (n = 1)--H-Mr AP activities ranged from 3.1-40.4 U/L and 3.1-16% of total serum AP activity. In sera of patients with leukemia (n = 18) H-Mr AP was not detectable. After the treatment of the sera with papain and phosphatidylinositol-specific phospholipase C, which release membrane-associated AP from membrane particles, H-Mr AP was no longer detectable. These results indicate that H-Mr AP in the sera of patients with solid tumors may derive from increasing cell shedding of the tumor cells with elevated levels of membrane fragments in serum, which is a well known phenomenon in liver tumors. H-Mr AP was not more detectable in the serum after successful tumor treatment. These data suggest that H-Mr AP was produced by the tumors and that this parameter may be a serological marker for some solid tumors even in the presence of normal total AP serum activity.

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Section: Discussionmentioning

confidence: 96%

“…Liquid Chromarography: Sampie preparation, separation and AP isoenzyme detection were described previously (7,8).…”

Section: Methodsmentioning

confidence: 99%

High-Molecular-Mass or Macromolecular Alkaline Phosphatase in Sera of Children with Solid Tumors

Schönaü

Beck

et al. 1994

Klin Padiatr

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Transient hyperphosphatasaemia of infancy

1988

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In five patients with benign transient hyperphosphatasaemia (THP), high activities of so-called "atypical" alkaline phosphatase or fragment isoenzymes were detected. One case occurred after rotavirus infection. Incubation with neuraminidase suggested that "atypical" alkaline phosphatase originated from highly glycosylated bone and liver isoenzymes. This may have been due to virus-induced low isoenzyme clearance from serum. The course of isoenzyme activities in THP following rotavirus infection was followed. Determination of atypical alkaline phosphatase may be useful in the diagnosis of THP.

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Clinical Relevance of Alkaline Phosphatase Isoenzyme Determinations by High Performance Liquid Chromatography

Cited by 2 publications

References 10 publications

High-Molecular-Mass or Macromolecular Alkaline Phosphatase in Sera of Children with Solid Tumors

High-Molecular-Mass or Macromolecular Alkaline Phosphatase in Sera of Children with Solid Tumors

Transient hyperphosphatasaemia of infancy

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