Clinical Relevance of Subclass Deficiency Diseases

Hanson, Lars Å.; Hahn-Zoric, Mirjana; Friman, Vanda; Theman, K.; Björkander, Janne; Lucas, Alexander H.; Nagao, A. T.

doi:10.1007/978-3-642-78438-5_9

Cited by 3 publications

(2 citation statements)

References 49 publications

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“…On the other hand, some of the twins, who did not respond to vaccination when judged by lEF-affinity immunobiot, had a good vaccination response when judged by ELISA. One reason for such differences might be that the different monoclonals against IgG2 used in the ELISA and to develop the bands after immunoblotting vary strikingly in their capacity to pick out various clones as recently demonstrated [18]. When different IgG2 antisera were used in the ELISA and in the lEF-affinity immunoblot some clones may have been missed in either method.…”

Section: Discussionmentioning

confidence: 99%

Differences within Mono‐ and Dizygotic Twin‐Pairs in Spectrotypes and Clones of IgG2 Antibodies to Pneumococcal Polysaccharide Type 1 and C‐Polysaccharide After Vaccination

et al. 1994

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Spectrotypes and clones of antibodies against pneumococcal capsular polysaccharide (Ps) type 1 and C-polysaccharide (C-Ps) were determined before and after immunization with a polyvalent pneumococcal Ps vaccine in 84 mono- or dizygotic twins. The method used was a micromodification of a rapid isoelectric focusing-affinity (IEF-affinity) immunoblot technique in agarose permitting characterization of isotype, light chain and Gm type. After vaccination the anti-type 1 Ps + anti-C-Ps clones were different in 75% of the monozygotic and 79% of the dizygotic twins. The anti-type 1 Ps clones differed among 72% of the monozygotic and 85% of the dizygotic twins (P > 0.05). Each twin had from zero to three clones producing IgG2 antibodies against type 1 Ps. A total of six different clones could be distinguished among all the twins. Vaccination enhanced the already actively secreting B-cell clones in 56 twins and newly recruited clones in 11 of the 84 twins; six among the 48 mono- and five among the 36 dizygotic twins. These new clones differed among the twins. Spectrotypes varied between all twins within the pairs. The fact that all twins differed in spectrotype is due to post-translational microheterogenity of the antibodies, events which are thus not genetically determined. The observation that even monozygotic twins possessed and responded with different clones within the pairs indicates that the V-region genes, which determine the final specificity of B cells, either differ from the original germ-line V region genes, e.g. owing to hypermutations or junctional diversity, or the rearranged germ-line genes occur accidentally although highly restricted.

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Section: Discussionmentioning

confidence: 99%

Differences within Mono‐ and Dizygotic Twin‐Pairs in Spectrotypes and Clones of IgG2 Antibodies to Pneumococcal Polysaccharide Type 1 and C‐Polysaccharide After Vaccination

et al. 1994

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“…Another implication might be an increased inflammation due to more antibody clones reacting with P. aeruginosa antigen and aggravating the immune complex disease (1 S), as is indicated by the correlation between ELISA levels and number of clones. In recent years it has become clear that antibody and T-cell responses to the human heat shock protein hsp60 and to bacterial 60-kDa heat shock proteins are important in connection with some autoimmune diseases such as insulin-dependent diabetes ( I 7,19) and arthritis (2). We found a correlation between the number of IgG1 and IgG2 antibody clones, as determined by IEF/affinity immunoblotting, and the level of anti-P. ueruginosa GroEL antibodies determined in C F patients by ELISA.…”

Section: Discussionmentioning

confidence: 99%

The clonal antibody response to Pseudomonas aeruginosa heat shock protein is highly diverse in cystic fibrosis patients

Ulanova

Petersen

Ciofu

et al. 1997

APMIS

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M., Hanson, L. 8. & Heriby, N. The clonal antibody response to Pseudomonas aeruginosa heat shock protein is highly diverse in cystic fibrosis patients. APMIS 105: 449456, 1997.The GroEL protein of Pseudoomonas aeruginosa belongs to the bacterial 60-65 kDa heat shock protein family. A strong antibody response to GroEL has been found in cystic fibrosis (CF) patients with chronic pulmonary infection caused by P. aeruginosu. Clonotypes of IgGl and IgG2 antibodies against GroEL were studied in 60 consecutive sera from 18 C F patients with chronic P. aeruginosa infection using isoelectric focusing in combination with affinity immunoblotting. The persistent antigenic stimulation in CF patients with chronic P. aeruginosa infection induced numerous IgGl and particularly IgG2 antibody clones against GroEL. The appearance of new clones with time reflected the long duration of the chronic infection. A striking addition of new clonotypes during the observation period occurred when a new unrelated bacterium (Burkholderia cepacia) had become established as a cause of the pulmonary infection, or when the P. aeruginosa infection became chronic.

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Humoral immune response to pneumococcal vaccination

Konradsen

1996

APMIS

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Clinical Relevance of Subclass Deficiency Diseases

Cited by 3 publications

References 49 publications

Differences within Mono‐ and Dizygotic Twin‐Pairs in Spectrotypes and Clones of IgG2 Antibodies to Pneumococcal Polysaccharide Type 1 and C‐Polysaccharide After Vaccination

Differences within Mono‐ and Dizygotic Twin‐Pairs in Spectrotypes and Clones of IgG2 Antibodies to Pneumococcal Polysaccharide Type 1 and C‐Polysaccharide After Vaccination

The clonal antibody response to Pseudomonas aeruginosa heat shock protein is highly diverse in cystic fibrosis patients

Humoral immune response to pneumococcal vaccination

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